Leica TCS SP8 SMD User Manual page 189

For fcs, flim and flcs
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1. First, switch on the APDs electrically.
NOTICE
Light that is too intense may damage the APDs
Never switch on the APDs under illumination. Otherwise, the detectors
can be damaged irreparably.
2. Now activate the detectors APD1 and/or APD2 (Figure 167, item 1) in Beam Path Setting
of LAS AF. Set the external port X1 to --- (Figure 167, item 2).
Figure 167:
Settings for acquiring a reference image using APDs
3. If the audible warning signal for overload of the APDs sounds or the APDs shut off,
reduce the illumination intensity immediately or switch off the APDs on the APD detector
unit or on the DSN electrically. It is not sufficient to deactivate the APDs in Beam Path
Settings.
4. Switch the MPD APDs back on manually. Activation of the MPD APDs takes about 20
seconds. The PE APDs switch on again automatically.
5. Start a continuous scan by clicking the image acquisition button Live (Figure 167, item 3)
or start an individual image acquisition by clicking the image acquisition button Capture
Image (Figure 167, item 4). Start with low laser intensity.
F(L)CS Data Acquisition
189

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