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Selecting the Laser
4. Adjust the laser intensity on the laser driver in order to obtain the shortest laser pulse
length (see Chapter 17.12 with respect to measuring instrument response time – "IRF"). In
a second step, adjust the laser intensity on the laser or the laser coupling module (LCU)
(Chapter 9.6), to carry out a corresponding adjustment with respect to the correct
fluorescence count rate for FLIM detection.
5. Close the shutter of the continuous wave VIS laser in Beam Path Settings and move all
AOTF sliders to the 0 position.
6. Open the shutter for pulsed lasers by turning the button to ON (Figure 104, item 1).
7. Activate the desired laser by moving the corresponding slider up all the way (Figure 104,
item 2).
In the control field of the pulsed lasers, the slider setting does not affect the laser intensity.
The laser intensity is controlled outside the LAS AF on the laser driver (PDL800, SEPIA) and
on the laser coupling module.
8. Make sure that the multifunction port (MFP, Figure 104, item 3) is in the right position
(Table 18).
Figure 104:
122
Notes about laser intensity
For intensities under 3.5 (PDL) or 35 % (SEPIA II), the pulsed output of the
laser light can be interrupted.
Do not select too high of values on the laser driver (PDL or SEPIA) unless
the laser intensity is not sufficient. High values increase the laser pulse
length.
Observe the user manuals provided
Always observe all of the user manuals provided for the individual
components and peripheral devices.
Settings for pulsed VIS lasers in Beam Path Settings
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