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Figure 180:
Setting of multiple FCS measuring points in a previously acquired xyz image stack
To set the FCS measuring points for FCS measurement time series, proceed as follows:
1. Take an xy or xz image or take an xyz or xzy stack.
2. Select the instrument parameter setting for the FCS measurement in the Setup FCS step.
3. Move to the Measurements step in the FCS Wizard. If necessary, you can fine-tune the
laser intensity here (Figure 179).
4. In the Experiments tab, check that the current image or xyz or xzy stack has been
activated.
5. Select the first measuring point in the image or stack in the image display.
6. Click Add (Figure 179).
7. To document the measuring point, right-click in the image and then click Snapshot.
F(L)CS Data Acquisition
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