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TCS SP5 X
User Manuals: Leica TCS SP5 X Laser Scanning Microscope
Manuals and User Guides for Leica TCS SP5 X Laser Scanning Microscope. We have
4
Leica TCS SP5 X Laser Scanning Microscope manuals available for free PDF download: User Manual, Manual
Leica TCS SP5 X User Manual (166 pages)
Brand:
Leica
| Category:
Microscope
| Size: 2 MB
Table of Contents
Table of Contents
3
Notes about this Manual
7
Copyright
7
General
9
About These Operating Instructions
9
Proper Intended Use
11
Legal Notes
13
Disclosure
13
Revisions and Changes
14
Warranty
14
Patents
14
Trademarks
16
Software Licenses
16
TCS SP5 Specifications
17
System Overview
17
Dimensions
18
System with Inverted Stand
18
System with Upright Stand
19
Installation Site Requirements
19
Permissible Ambient Conditions
20
Electrical Connections
20
Required Cooling Capacity
22
Important Additional Notes
22
Required Safety Measures for VIS and UV Systems
23
Required Laser Safety Measures for MP Systems
23
Important Additional Notes
23
Safety Notes
25
Which Standards Does this Product Meet
25
Which Laser Class Does the Product Have
27
Laser Class for VIS and UV Systems
27
Laser Class for MP Systems
27
Warnings, Safety Cautions, and Notes
28
What Should the User of the Laser Scanning Microscope Observe
29
Safety Notes for the User
31
Safety Notes for Operation
32
Specific Safety Notes
36
Operational Reliability
36
De-Energizing the System
36
Maximum Current Load of the Power Outlet Strip at the Supply Unit
37
Laser Safety Devices
38
Eye Protection for VIS or UV Systems
38
Detachable-Key Switch
39
Emissions Warning Indicators
40
Remote Interlock Connector on the Supply Unit
42
Remote Interlock Connector on the External 405Nm Advanced Laser
43
Remote Interlock Connectors on Additional External Lasers
43
Interlock Connection on Scanner
45
Function and Position of Safety Switches
46
Transmitted-Light Lamp Housing for Inverted Stands
46
Transmitted-Light Lamp Housing for Upright Stands
48
Mirror Housing on Upright Stand
49
Special Laser Safety Equipment
50
Safety Beam Guide and Beam Collector
50
Eye Protection for MP Systems
51
Shielding in MP Systems (UV Laser)
52
Overview of Usable VIS/UV Lasers
53
Overview of Usable MP Lasers (IR)
54
Safety Label on TCS SP5 System
55
Inverted Stand DMI 4000/6000 CS
55
Upright Stand DM 5000/6000 CS
56
Scan Head
59
Supply Unit
60
External 405-Nm Laser Advance / 405-Nm Imaging Laser
61
External 488-Nm Laser
62
Cover (for Replacement Flange)
64
Requirements Related to the Installation/Storage Location
65
Changing the Installation Site
66
Scanner Cooling
67
Care and Cleaning
69
Cleaning the Optical System of the Microscope
69
Cleaning the Microscope Surface
70
Startup of the System
73
Setting up Users
76
Starting the las AF
81
Introduction to las AF - Help
85
General
85
Calling Online Help
85
Structure of the Online Help
86
Imprint
87
In the Respective Context (Context-Sensitive)
88
Via the Help Menu
88
Full-Text Search with Logically Connected Search Terms
89
Key Combinations
90
Structure of the User Interface
91
General Structure of the User Interface
91
What Is the Köhler Illumination
93
Setting the Köhler Illumination
94
Introduction to Confocal Work
97
Preparation
97
The Objective
98
Conventional Microscopy
99
Why Scan
102
How Is an Optical Section Created
104
Acquiring Optical Sections
106
Data Acquisition
107
Illumination
109
Beam Splitting
110
Emission Bands
110
The Pinhole and Its Effects
112
Image Detail and Raster Settings
114
Signal and Noise
119
Profile Cuts
122
Multiparameter Fluorescence
123
Illumination
123
Beam Splitting
124
Emission Bands
125
Crosstalk
125
Sequential Capture
127
Unmixing
127
Series
130
Z-Stack
130
About Section Thickness
130
And Spacing
131
Data Volumes
131
Depictions
133
Gallery
133
Movie
134
Orthogonal Projections
134
Rotated Projections
135
Time Series
137
About Scan Speeds
137
Points
138
Lines
138
Planes
138
Spaces (Time-Space)
139
FRAP Measurements
139
Spectral Series
140
Data Acquisition and Utilization
140
About Spectral Resolution
140
Combinatorial Analysis
141
Switching off the System
143
Contact
145
Declaration of Conformity
147
Glossary
149
Safety Data Sheets
159
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Leica TCS SP5 X User Manual (123 pages)
Brand:
Leica
| Category:
Microscope
| Size: 15 MB
Table of Contents
Notes about this Manual
7
Patents
8
General
9
TCS SP5 Specifications
11
Dimensions
12
Requirements Regards Ambient Conditions
13
Electrical Connections
14
Safety Instructions and Their Meaning
15
Safety Instructions
17
Which Laser Class Does the Product Have
18
Laser Class for MP Systems
19
Safety Notes for the User
20
Safety Notes for Operation
21
Specific Safety Notes
23
De-Energizing the System
24
Laser Safety Devices
25
Emissions Warning Indicators
26
Remote Interlock Connector on the Supply Unit
27
Remote Interlock Connectors on Additional External Lasers
28
Function and Position of Safety Switches
29
Transmitted-Light Lamp Housing for Inverted Stands
30
Transmitted-Light Lamp Housing for Upright Stands
31
Mirror Housing on Upright Stand
32
Special Laser Safety Equipment
34
Eye Protection for MP Systems
35
Overview of Usable VIS/UV Lasers
36
Overview of Usable MP Lasers (IR Lasers)
37
Inverted Stand DMI 6000 CS
38
Upright Stand DM 5000/6000 CS
40
Scan Head
42
Supply Unit
43
MP Beam Coupling Unit
44
Cover (for Replacement Flange)
45
Mirror Housing
46
Installation and Storage Site Requirements
47
Scanner Cooling
48
Care and Maintenance
49
Cleaning the Microscope Surface
50
Startup of the System
51
Setting up Users
52
Starting up
53
Starting the las AF
55
Introduction to las AF
57
Calling Online Help
58
Full-Text Search with Logically Connected Search Terms
59
Structure of the User Interface
60
Key Combinations
61
Introduction to Confocal Work
63
Conventional Microscopy
64
Why Scan
67
How Is an Optical Section Created
68
Acquiring Optical Sections
70
Illumination
72
Beam Splitting
73
Emission Bands
74
The Pinhole and Its Effects
76
Image Detail and Raster Settings
77
Signal and Noise
81
Profile Cuts
83
Multiparameter Fluorescence
84
Beam Splitting
86
Sequential Capture
87
D Series
88
Distances
89
Depictions
90
Orthogonal Projections
91
Rotated Projections
92
Scan Speed
93
FRAP Measurements
94
Combinatorial Analysis
95
Switching off the System
97
Disposal
99
Contact
101
Declaration of Conformity
103
Glossary
105
Safety Data Sheets
111
Leica TCS SP5 X User Manual (50 pages)
Brand:
Leica
| Category:
Microscope
| Size: 5 MB
Table of Contents
Table of Contents
3
1 General
9
Copyright
9
About this Operating Manual
10
2 Leica TCS SP5 / TCS SP5 X
11
TCS SP5 System Overview
11
TCS SP5 X System Overview
12
Intended Use
12
Conformity
13
Patents
14
Serial Number
14
Specifications
15
Dimensions
15
TCS SP5 with Inverted Microscope
15
TCS SP5 with Upright Microscope
15
TCS SP5 X with Inverted Microscope
16
TCS SP5 X with Upright Microscope
16
Electrical Connection Requirements
17
Electrical Connection Requirements of Supply Unit
17
Electrical Connection Requirements of Achromatic Light Laser
17
Requirements Regarding Ambient Conditions
18
Permitted Ambient Conditions
18
Waste Heat/Required Cooling Performance
19
Features
19
Overview of Usable VIS/UV Lasers
19
Overview of Usable MP Lasers (IR Lasers)
20
Picosecond Laser
20
Femtosecond Laser
21
Overview of Usable VIS/UV Lasers for TCS SP5 X
22
Which Laser Class Does the Product Have
23
Required Laser Safety Measures
23
3 Safety Instructions and Their Meanings
25
4 General Safety Instructions
27
Laser Class for VIS and UV Systems
27
Laser Class for MP Systems
27
What Does the Owner/Operator Have to Observe
27
Safety Instructions for the User
29
Operational Reliability
29
Maximum Current Load of the Multiple Socket Outlet at the Supply Unit
30
5 Safety Devices
31
Disconnecting the Power Supply
31
Detachable-Key Switch
32
Emissions Warning Indicators
34
Remote Interlock Connection on the Supply Unit
35
Remote Interlock Connection on the Achromatic Light Laser
36
Remote Interlock Connections on External Lasers
37
Remote Interlock Jack/Interlock Connector on the Scanner
37
Function and Position of Safety Switches
38
Special Laser Safety Equipment
39
Laser Protection Tube and Beam Stop
39
Shielding in MP Systems (IR Lasers)
40
Safety Labels on the System
41
Inverted Microscope DMI 6000 CS
41
Upright Microscope DM 5000/6000 CS
43
Scan Head
45
Achromatic Light Laser
46
External UV Laser
47
Supply Unit
48
MP Beam Coupling Unit
49
Cover (for Replacement Flange)
50
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Leica TCS SP5 X Manual (14 pages)
Brand:
Leica
| Category:
Microscope
| Size: 0 MB
Table of Contents
Table of Contents
2
Anatomy of the Leica Sp5 Confocal Microscope
3
Initialisation Procedure
4
Sp5 Microscope
5
Right Side of Microscope (Detailed View)
5
Front Side of Microscope (Detailed View)
6
Left Side of Microscope (Detailed View)
6
Control Panel Buttons
7
Starting the las Software
8
Configure for Acquisition
8
The Left Panel Allows You to Configure the Image
9
The Right Panel Allows You to Load a Pre-Made
10
Configuration or to Create Your Own One
10
Create a Sequential Acquisition
10
Acquire Images
11
ACQUIRING a Z-Stack
12
Save and Export Your Images
13
Shutdown Procedure
14
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