Leica TCS SP8 SMD User Manual page 123

For fcs, flim and flcs
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Configuration
405
405+440
405+470
405+470+640
440
440+470
470
470+640
Table 18:
MFP settings based on the excitation wavelength used
You can use pulsed VIS lasers together with a pulsed UV laser. The MFP setting is not
influenced by UV. It is defined by the selection of the pulsed VIS lasers.
Combination pulsed and continuous wave VIS excitation
Do not use any pulsed VIS excitation together with continuous wave VIS
excitation for continuous wave laser lines close to the wavelengths of
the pulsed laser (such as the combination 470 nm pulsed + 488 nm
continuous wave), as no optimum MFP setting exists for such a
combination.
9. If you work on a beam splitter system, set the beam splitter to Substrate in the Beam Path
Settings in LAS AF (Figure 105). In case you want to use a pulsed laser combined with a
continuous wave laser line, chose the corresponding main beam splitter that reflects the
continuous wave laser line into the scan head.
Figure 105:
Setting the beam splitter to substrate
Lasers used
MFP settings in LAS AF
405
Substrate
405
Substrate
440
RSP 445
405
Substrate
405+470
SD 470
470
SD 470
405
Substrate
405+470
DD 470+640
405+640
DD 470+640
470
DD 470+640
470+640
DD 470+640
640
DD 470+640
440
RSP 445
440
RSP 445
470
SD 470
470
RSP 445
470
DD 470+640
470+640
DD 470+640
640
DD 470+640
Selecting the Laser
123

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