Leica TCS SP8 SMD User Manual page 205

For fcs, flim and flcs
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• Number of defined points: The user does not have access to this field. It serves to
visualize the number of measuring points defined in the viewer (Figure 181, item 6).
The maximum number of FCS measurements in a measurement series is 1000.
Figure 181:
Definition of an FCS Time Series
The following functions are available for repeated processing of all points:
• Number of cycles: A Cycle means that, in the sequence of their definition, the individual
measuring points are processed exactly once in the image display with the number of
repetitions specified in the Definition of point measurements dialog. The input field
enables you to specify the repetitions to be carried out relative to a complete cycle
(Figure 181, item 7). The maximum number of cycles is 1000.
• Cycle interval: Specifies the time between the beginning of one cycle and the next cycle
(Figure 181, item 8). If the Minimize box is checked, processing cycles will be carried out
as fast as possible (Figure 181, item 9).
• Image after each __ cycles: If you enable check boxes and enter the number 1, an image
or stack is acquired with the current image acquisition settings after each cycle. If you
enter the number 2, an image or stack is acquired after every second cycle; if you enter
3, after every third cycle, and so on. After processing all cycles, one last image or stack
will be acquired (Figure 181, item 10).
F(L)CS Data Acquisition
205

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