Leica TCS SP8 SMD User Manual page 111

Figure 90: Overview of the SMD FCS Wizard steps
• "Setup Corr-ring" step The recommended FCS objective (63x Plan Apo 1.2 W) has a
correction ring to optimally correct for differences in the refractive index between
immersion and specimen and temperature effects. An optimum adjustment of the
correction ring correlates with the best possible optical resolution of the system – a
basic requirement for FCS measurements. In the Setup Corr-ring step, the beam path is
preconfigured for a fast and efficient adjustment of the correction ring.
• Setup Imaging workstep: Here, you can establish and optimize the parameters for scan
acquisition. This step also includes a semi-automated routine for fine-tuning the park
position of the scanner (Chapter 18.1.2).
• Setup FCS step: Here, you can define and optimize instrument parameters for the FCS
measurement. The FCS measurement instrument parameters (such as selection of the
laser line and intensity, pinhole size, etc.) are managed independently of the parameters
for image acquisition. The FCS settings can also be saved and edited. To optimize the
measurement conditions, you can use the crosshair to define an FCS measurement point
in the previously acquired xy or xz image or an xyz or xzy stack, and carry out a test
measurement there.
LAS AF
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