Leica TCS SP8 SMD User Manual page 161

Figure 145: Select the detector in Beam Path Settings
9. Define the stack in the Λ stack dialog as follows (Figure 146):
• Excitation Begin and Excitation End: Excitation wavelength of the first and last FLIM
image
• No. of Excitation Steps: Number of FLIM images that are taken from the different
excitation wavelengths
Λ
Excitation Stepsize: Step size of the excitation wavelength from one image to the
•
next
Figure 146: Defining xy
Λ
FLIM stacks
FLIM Data Acquisition
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