Leica TCS SP8 X User Manual
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Leica TCS SP8 X User Manual

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Living up to Life
User Manual
Leica TCS SP8
Leica TCS SP8 MP
Leica TCS SP8 X
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Summary of Contents for Leica TCS SP8 X

  • Page 1 Living up to Life User Manual Leica TCS SP8 Leica TCS SP8 MP Leica TCS SP8 X...
  • Page 2 Published by: Leica Microsystems CMS GmbH Am Friedensplatz 3 D-68165 Mannheim (Germany) http://www.leica-microsystems.com http://www.confocal-microscopy.com Responsible for contents: Leica Microsystems CMS GmbH Copyright © Leica Microsystems CMS GmbH. All rights reserved.

  • Page 3: Copyright

    Copyright Copyright All rights to this document are held by Leica Microsystems CMS GmbH. Adaptation, translation and reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica Microsystems CMS GmbH.
  • Page 4 Copyright...

  • Page 5: Table Of Contents

    TCS SP8 X System Variants ........
  • Page 6 TCS SP8 X with inverted microscope ........35...
  • Page 7 Contents 9.2.3.2 Femtosecond laser..........51 10 Safety Features .
  • Page 8 Contents 11.10 External UV Laser............76 11.11 Other External Lasers .
  • Page 9 Contents 23 Contact ..............125 24 Appendix.
  • Page 10 Contents...

  • Page 11: About This User Manual

    If you have any suggestions or improvements for this User Manual, please contact the Leica branch office in your country.
  • Page 12 About this User Manual...

  • Page 13: Intended Use

    The manufacturer assumes no liability for damage caused by, or any risks arising from, use of the microscopes for purposes other than those for which they are intended, or not using the microscopes within the specifications of Leica Microsystems CMS GmbH. In such cases, the Declaration of Conformity shall be invalid.
  • Page 14 Intended Use...

  • Page 15: Liability And Warranty

    Figures are for illustration purposes. The system you purchased can deviate from the illustrations without Leica Microsystems CMS GmbH explicitly specifying such. Leica Microsystems CMS GmbH shall not be liable for any injury or property damage caused by untrained or unauthorized persons.
  • Page 16 Liability and Warranty...

  • Page 17: Meaning Of The Warning Messages In The Manual

    Meaning of the warning messages in the manual Meaning of the warning messages in the manual WARNING Electric shock This warns you of hazardous electrical voltage. Following the instructions is mandatory, since otherwise there is a risk of severe or fatal injury.
  • Page 18 Meaning of the warning messages in the manual CAUTION Injuries from… This note warns you of minor to moderate injuries that can be prevented by following instructions. NOTICE Risk of damage to the system This note describes possible material damage that can occur in case of misuse.

  • Page 19: General Safety Notes

    Do not open these crates. The crates may be opened and unpacked by Leica service technicians or by people who are authorized by Leica Microsystems CMS GmbH only. • The system may only be set up by Leica service technicians or by people who are authorized by Leica Microsystems CMS GmbH.

  • Page 20: Safety Devices And Safety Labels

    • Do not connect any external equipment or other components. If you have questions, please directly contact the Leica branch office in your country. Safety Devices and Safety Labels •...

  • Page 21: Electrical Safety

    S70 microscope condenser could result in a hazard from laser radiation. • Only use S1 and S28 Leica microscope condensers. • Do not look into the eyepieces during the scanning operation. • Never change samples during a scanning operation.

  • Page 22: Contact With Liquids

    • The indicator continues to be lit after being switched off using the keyswitch • Scanning of the specimen is not activated after being switched on properly (laser radiation in the specimen area). If any of these occur, immediately notify the Leica branch office in your country or your local contact person.

  • Page 23: Additional Notes On Handling The System

    • We recommend using a room that can be completely darkened. • Do not expose the system to drafts. • If the system has to be moved to a new location for any reason, contact the Leica branch office in your country.

  • Page 24: Protecting The System

    LED on the detector module (see Chapter 12.3, Figure 75, item 2) lights up. • If you have any further questions, please directly contact the Leica branch office in your country (see Chapter "Contact").

  • Page 25: System Overview And Properties

    System Overview and Properties System Overview and Properties TCS SP8 System Variants 7.1.1 TCS SP8 with upright microscope and compact supply unit Figure 2: TCS SP8 with upright microscope and compact supply unit 1 Upright microscope 5 Compact supply unit 2 Scan head 6 Control panel 3 SmartMove...
  • Page 26 System Overview and Properties Figure 3: Dimensions of TCS SP8 with upright microscope and compact supply unit...

  • Page 27: Tcs Sp8 With Upright Microscope And Flexible Supply Unit

    System Overview and Properties 7.1.2 TCS SP8 with upright microscope and flexible supply unit Figure 4: TCS SP8 with upright microscope and flexible supply unit 1 Upright microscope 6 Flexible supply unit 2 Scan head 7 Control panel 3 SmartMove 8 Fluorescence lamp EL6000 4 Workstation 9 Microscope electronics box...

  • Page 28: Tcs Sp8 With Inverted Microscope And Compact Supply Unit

    System Overview and Properties 7.1.3 TCS SP8 with inverted microscope and compact supply unit Figure 6: TCS SP8 with inverted microscope and compact supply unit 1 Inverted microscope 5 Control panel 2 SmartMove 6 Fluorescence lamp EL6000 3 Workstation 7 Microscope electronics box 4 Compact supply unit 8 Scan head Figure 7:...

  • Page 29: Tcs Sp8 With Inverted Microscope And Flexible Supply Unit

    System Overview and Properties 7.1.4 TCS SP8 with inverted microscope and flexible supply unit Figure 8: TCS SP8 with inverted microscope and flexible supply unit 1 Inverted microscope 6 Control panel 2 SmartMove 7 Fluorescence lamp EL6000 3 Workstation 8 Microscope electronics box 4 Main switch board 9 Scan head 5 Flexible supply unit...

  • Page 30: Tcs Sp8 Mp System Variants

    System Overview and Properties TCS SP8 MP System Variants 7.2.1 TCS SP8 MP with DM6000 and IR laser without Dispersion Compensation Figure 10: System overview of the TCS SP8 MP with DM6000 and IR laser without dispersion compensation 1 Stage with supply unit (compact or flexible) 4 Beam coupling unit 5 IR laser without dispersion compensation 2 Optical table, 90 x 120 cm (2'11"...

  • Page 31: Tcs Sp8 Mp With Dm6000 And Ir Laser With Dispersion Compensation

    System Overview and Properties 7.2.2 TCS SP8 MP with DM6000 and IR Laser with Dispersion Compensation Figure 11: System overview of the TCS SP8 MP with DM6000 and IR laser with dispersion compensation 1 Stage with supply unit (compact or flexible) 4 Beam coupling unit 5 IR laser with dispersion compensation 2 Optical table, 180 x 120 cm (5'11"...

  • Page 32: Tcs Sp8 Mp With Dmi6000 And Ir Laser Without Dispersion Compensation

    System Overview and Properties 7.2.3 TCS SP8 MP with DMI6000 and IR Laser without Dispersion Compensation Figure 12: System overview of the TCS SP8 MP with DMI6000 and IR laser without dispersion compensation 1 Stage with supply unit (compact or flexible) 3 Microscope DMI6000 4 Beam coupling unit 2 Power supply and cooling of the IR laser...

  • Page 33: Tcs Sp8 Mp With Dmi6000 And Ir Laser With Dispersion Compensation

    System Overview and Properties 7.2.4 TCS SP8 MP with DMI6000 and IR Laser with Dispersion Compensation Figure 13: System overview of the TCS SP8 MP with DMI6000 and IR laser with dispersion compensation 1 Stage with supply unit (compact or flexible) 4 Beam coupling unit 5 IR laser with dispersion compensation 2 Optical table, 180 x 120 cm (5'11"...

  • Page 34: Tcs Sp8 X System Variants

    6 Flexible supply unit 2 Scan head 7 Control panel 3 SmartMove 8 Fluorescence lamp EL6000 4 Workstation 9 Microscope electronics box 5 Main switch board 10 White light laser Figure 15: Dimensions of TCS SP8 X with upright microscope...

  • Page 35: Tcs Sp8 X With Inverted Microscope

    6 Control panel 2 SmartMove 7 Fluorescence lamp EL6000 3 Workstation 8 Microscope electronics box 4 Main switch board 9 Scan head 5 Flexible supply unit 10 White light laser Figure 17: Dimensions of TCS SP8 X with inverted microscope...

  • Page 36: Tcs Sp8 With External Uv Laser

    System Overview and Properties TCS SP8 with external UV laser Figure 18: Overview with dimensions of TCS SP8 with external UV laser 1 Workstation 4 Power supply (external UV laser) 2 Stage with supply unit 5 External UV laser 3 Microscope stage with scan head Controls on the Supply Unit 7.5.1 Main switch board on the flexible supply unit...

  • Page 37: Control Panel Field On The Compact Supply Unit

    TCS SP8 MP with DMI6000 and IR Laser Figure 13 with Dispersion Compensation TCS SP8 X with upright microscope 280 x 110 x 175 cm (9'2" x 3'7" x 5'9") TCS SP8 X with inverted microscope 303 x 110 x 140 cm (9'11" x 3'7" x 4'7") TCS SP8 with external UV laser and 362 x 110 x 175 cm (11'11"...

  • Page 38: Weight Of The Supply Unit

    System Overview and Properties 7.6.2 Weight of the supply unit Compact Supply Unit 40 kg (88 lbs) Flexible Supply Unit 90 kg (198 lbs) 7.6.3 Electrical specifications Flexible Supply Unit Compact Supply White light laser Unit Supply voltage 100 V~ to 240 V~ ± 10%, grounded Power consumption 2x 1600 VA 700 VA...

  • Page 39: Serial Number

    System Overview and Properties radio transmitting devices such as DECT phones can cause picture interference if used in the immediate vicinity of the system. Serial Number The serial number for your system is located on the rear side of the scan head: Figure 21: Rear side of the scan head –...
  • Page 40 System Overview and Properties...

  • Page 41: Ambient Conditions

    Otherwise, this could cause fire and electrical shocks to the system and the electrical components. • The room should be equipped with a telephone connection to contact Leica Microsystems CMS GmbH for phone support. For RemoteCare, an Internet connection is also needed.

  • Page 42: Room Dimensions

    TCS SP8 MP with DMI6000 and 500 x 280 cm (16'5" x 9'2") IR laser with precompensation TCS SP8 X with upright 400 x 280 cm (13'1" x 9'2") microscope TCS SP8 X with inverted 423 x 280 cm (13'10" x 9'2"...

  • Page 43: Electrical Connection Requirements

    Ambient Conditions Electrical Connection Requirements WARNING Electric shock is possible when using ungrounded sockets This system is designed for connection to grounded (earthed) outlets. The grounding plug performs an important safety function. To avoid the risk of electrical shock or damage to the instrument, do not disable this feature.

  • Page 44: Tcs Sp8 Mp With Flexible Supply Unit

    Depending on the system configuration, further electrical circuits may be required. Power supply for USA Terminals of type NEMA 5-15 When using HyD RLDs An additional power supply 8.4.5 TCS SP8 X Supply voltage 100 V~ to 240 V~ ± 10%, grounded Frequency 50/60 Hz...

  • Page 45: External Lasers

    Ambient Conditions Power connection Three separate electric circuits with Power supply voltage: 100 V - 120 V, fuse protection: 20 A Power supply voltage: 200 V - 240 V, fuse protection: 12 - 16 A (Two for the flexible supply unit's power supply and one for supplying peripheral devices) To prevent damage to the instrument, the two types of voltage (200 to 240 V~ and 100 to 120 V~) must not be...

  • Page 46: Waste Heat And Cooling

    Ambient Conditions Figure 22: Load capacity on the rear side of the multiple socket outlet on the flexible supply unit Waste Heat and Cooling NOTICE Instruments may sustain damage Never install the system close to air conditioners or other ventilation systems.

  • Page 47: Tcs Sp8 With Compact Supply Unit

    Ambient Conditions The remaining waste heat (1.6 kW) must be extracted; otherwise the room temperature will rise. 8.6.2 TCS SP8 with compact supply unit Waste heat of the complete 1.7 kW system with a compact supply unit 8.6.3 TCS SP8 MP with flexible supply unit Waste heat of the complete 6.2 kW system, including the argon...

  • Page 48: Tcs Sp8 X

    Ambient Conditions 8.6.5 TCS SP8 X Waste heat of the complete 3.6 kW system, including the argon laser and white light laser Waste heat of the argon laser 1.6 kW For cooling, the argon laser must be connected to the provided argon laser fan. The...

  • Page 49: Laser

    Laser Laser It is mandatory to observe the laser safety measures for laser class 3B / IIIb (VIS and UV lasers) or for laser class 4 / IV (MP systems) in accordance with applicable national and federal regulations. You may only use the laser listed here, as the laser safety devices are only designed for the laser variants listed here.

  • Page 50: Vis/Uv Lasers For Tcs Sp8 X

    < 5 Continuous wave (cw) Diode 638 Continuous wave (cw) 9.2.2 VIS/UV Lasers for TCS SP8 X The TCS SP8 X features a combination of the lasers listed below: Laser type Wavelength Maximum Maximum Pulse duration (nm) light output at...

  • Page 51: Femtosecond Laser

    Laser 9.2.3.2 Femtosecond laser Laser type Wavelength Maximum Maximum Pulse duration (nm) light output at light output in laser output focal plane MaiTai fs 780 - 920 < 1,2 < 0,6 pulsed ~ 80 fs MaiTai HP fs 690 - 1040 <...
  • Page 52 Laser...

  • Page 53: Safety Features

    Safety Features Safety Features The Leica TCS SP8 system is available with a flexible or a compact supply unit. Note the safety features described here that apply to your supply unit. 10.1 Main Circuit Breaker for Disconnecting the Power Supply 10.1.1...

  • Page 54: Key Switch

    Safety Features 10.2 Key Switch 10.2.1 Master Key Switch on the Compact Supply Unit There is a master key switch on the compact supply unit for protection against unauthorized use of the laser products. This master key switch is integrated in the interlock circuit. If the master key switch is in the "off"...

  • Page 55: Key Switch For The White Light Laser

    Safety Features 10.2.3 Key Switch for the White Light Laser The external lasers can also be turned off individually. The detachable key switch for protection against unauthorized use of the external white light laser is located on the front of the white light laser (see Figure 26). Figure 26: Key switch for the white light laser 10.2.4...

  • Page 56: Key Switches For Other External Lasers

    Safety Features 10.2.5 Key Switches for Other External Lasers Observe the user manuals for external lasers Please refer to the information from the documents provided by the laser manufacturer for the external lasers. Pay particular attention to the laser manufacturer's notes! 10.3 Emission Warning Indicators The operational readiness of lasers is signaled by an emission warning indicator.

  • Page 57: Emission Warning Indicator At The White Light Laser

    Safety Features Figure 30: Emission warning indicator on the main switch board of the flexible supply unit 10.3.2 Emission Warning Indicator at the White Light Laser The emission warning indicator of the achromatic light laser is located on the front of the achromatic light laser (see Figure 31) and is red when lit.

  • Page 58: Emission Warning Indicator On Other External Lasers

    • Scanning of the specimen is not activated after being switched on properly (laser radiation in the specimen area). If any of these occur, immediately notify the Leica branch office in your country or your local contact person. 10.4 Interlock Connectors 10.4.1...
  • Page 59 Safety Features interlock can, for example. be connected to a door contact. When the door is opened, the laser beam is then interrupted automatically. The total length of the cable must not exceed 10 m. If a large amount of electromagnetic interference (EMC) is expected in the environment, use a shielded cable with a shielded plug.

  • Page 60: Interlock Connector On The White Light Laser

    Interlock Connector on the White Light Laser Interlock connector when using the white light laser If the white light laser is operated as a component of the TCS SP8 X system, you have to use the interlock connector on the supply unit! The shorting plug must be connected to the interlock connector of the white light laser.

  • Page 61: Interlock Connector On Other External Lasers

    Safety Features 10.4.3 Interlock Connector on Other External Lasers Observe the user manuals for external lasers Please refer to the information from the documents provided by the laser manufacturer for the external lasers. Pay particular attention to the laser manufacturer's notes! 10.4.4 Interlock Connector on the Scan Head The interlock connector is located on the rear side of the scan head (operating voltage: 12...

  • Page 62: Safety Switches On The Microscope

    LAS AF. For safety reasons, the shutters remain closed. No further use of the laser is possible. In this situation, the system must not be operated. Contact the Leica branch office in your country or your contact person.

  • Page 63: Special Laser Safety Equipment

    For safety reasons, the shutters remain closed. No further use of the laser is possible. In this situation, the system must not be operated. Contact the Leica branch office in your country or your contact person.

  • Page 64: Safety Beam Guide On The Tcs Sp8 Mp

    Safety Features Reordering a Condenser Base: When reordering a condenser base (see Figure 39, item 2), be sure to note that the condenser base is supplied without a laser protection shield (see Figure 39, item 3). The existing laser protection shield (see Figure 39, item 3) must always be reinstalled. Please consult the microscope's user manual provided.

  • Page 65: Safety Labels On The System

    Missing or damaged safety labels must be attached immediately and at the described location. Operation without the safety labels shown is not permitted. If you have any further questions, contact your laser safety officer or the Leica branch office in your country immediately.

  • Page 66: Inverted Microscope

    Safety Labels on the System 11.1 Inverted microscope Rear view of microscope: Figure 41: Safety labels for the inverted microscope Position System Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE WENN ABDECKUNG GEÖFFNET TO BEAM NICHT DEM STRAHL AUSSETZEN...
  • Page 67 Safety Labels on the System Front view of the microscope: Figure 42: Safety labels for the inverted microscope Position System Safety label in English Safety label in German LASER RADIATION IS EMITTED AUSTRITT VON FROM THIS APERTURE LASERSTRAHLUNG AVOID EXPOSURE BESTRAHLUNG VERMEIDEN UV/MP VISIBLE AND INVISIBLE LASER...

  • Page 68: Upright Microscope

    Safety Labels on the System 11.2 Upright Microscope Rear view of microscope: Figure 43: Safety labels for the upright microscope Position System Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE WENN ABDECKUNG GEÖFFNET TO BEAM NICHT DEM STRAHL AUSSETZEN...
  • Page 69 Safety Labels on the System Front view of the microscope: Figure 44: Safety labels for the upright microscope Position System Safety label in English Safety label in German LASER RADIATION IS EMITTED AUSTRITT VON FROM THIS APERTURE LASERSTRAHLUNG AVOID EXPOSURE BESTRAHLUNG VERMEIDEN 1 and 2 UV/MP...

  • Page 70: Scan Head

    Safety Labels on the System 11.3 Scan Head Front view of the scan head: Figure 45: Safety labels on the scan head...
  • Page 71 Safety Labels on the System Position System Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE WENN ABDECKUNG GEÖFFNET TO BEAM NICHT DEM STRAHL AUSSETZEN DANGER VORSICHT VISIBLE AND INVISIBLE LASER SICHTBARE UND UNSICHTBARE RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B...

  • Page 72: Compact Supply Unit

    Safety Labels on the System 11.4 Compact Supply Unit View of the compact supply unit: Figure 46: Safety labels on the compact supply unit Position Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE TO WENN ABDECKUNG GEÖFFNET NICHT BEAM...

  • Page 73: Mp Beam Coupling Unit

    Safety Labels on the System Position Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE TO WENN ABDECKUNG GEÖFFNET NICHT BEAM DEM STRAHL AUSSETZEN Table 7: Safety Labels on the Flexible Supply Unit (see Figure 47) 11.6 MP Beam Coupling Unit Angled front view of the right side of the MP beam coupling unit:...

  • Page 74: Cover For Replacement Flange

    Safety Labels on the System 11.7 Cover for replacement flange Front view of the cover: Figure 49: Safety labels on the cover Position System Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE WENN ABDECKUNG GEÖFFNET TO BEAM...

  • Page 75: Mirror Housing

    Safety Labels on the System 11.8 Mirror Housing Figure 50: Safety label on the mirror housing (top) Position System Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE WENN ABDECKUNG GEÖFFNET TO BEAM NICHT DEM STRAHL AUSSETZEN...

  • Page 76: White Light Laser

    Safety Labels on the System 11.9 White light laser Rear side of white light laser: Figure 51: Safety label on the rear side of the white light laser Position Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE TO WENN ABDECKUNG GEÖFFNET NICHT...

  • Page 77: Other External Lasers

    Safety Labels on the System 11.11 Other External Lasers Observe the user manuals for external lasers Please refer to the information from the documents provided by the laser manufacturer for the external lasers. Pay particular attention to the laser manufacturer's notes!
  • Page 78 Safety Labels on the System...

  • Page 79: Switching On The System

    Switching On the System Switching On the System The TCS SP8 and TCS SP8 MP systems are available with a flexible or compact supply unit. Observe the switch-on sequence that applies to your supply unit. NOTICE Damage is possible when contacting the specimen stage When using an inverted microscope, the illuminator arm must be swung back before the system start and LAS AF start because the stage can damage the condenser, the objective or the specimen during...
  • Page 80 Switching On the System has concluded. Figure 54: Overview of the main switch board on the flexible supply unit 4. Check whether the microscope is switched on. The microscope is operational once the readiness indicator (Figure 55, item 1) on the toggle switch is lit up. If the readiness indicator is not lit, activate the electronics box toggle switch (Figure 55, item 2).
  • Page 81 Switching On the System Figure 57: Switch on the NDD detection unit 7. Switch on the scan head on the flexible supply unit's main switch board (see Figure 54, item 2). 8. Switch on the lasers on the main switch board of the flexible supply unit (see Figure 54, item 3).
  • Page 82 Switching On the System WARNING Risk of permanent eye and skin damage from laser radiation From this time on, laser radiation may be present in the specimen area of the laser scanning microscope. Make sure to follow the safety notes for operation of the system.
  • Page 83 Switching On the System Figure 61: Power switch on external UV laser 405 14. Press the following key switches to switch on the external UV laser 355 including 405: Figure 62: Key switch on the power supply of external UV laser 355 Figure 63: Key switch on external UV laser 405 15.

  • Page 84: Tcs Sp8 With Compact Supply Unit

    Switching On the System Change password After logging on with your own user ID, you may change your password. To do so, press the Ctrl, Alt and Del keys at the same time. Then, click Change password. The Change password dialog box opens. Now you can start the LAS AF software.
  • Page 85 Switching On the System 3. Switch on the workstation. The operating system starts automatically. Wait until the startup process has concluded. 4. Turn on the microscope by actuating the electronics box toggle switch (Figure 66, item 2). The microscope is operational once the readiness indicator (Figure 66, item 1) on the toggle switch is lit up.
  • Page 86 Switching On the System Figure 69: Overview of the Control Panel Field on the Compact Supply Unit 8. Switch on the lasers on the front side of the compact supply unit (see Figure 69, item 2). The system's power supplies and fans are started. 9.
  • Page 87 Switching On the System Figure 71: Power switch on external UV laser 405 12. Press the following key switches to switch on the external UV laser 355 including 405: Figure 72: Key switch on the power supply of external UV laser 355 Figure 73: Key switch on external UV laser 405 13.

  • Page 88: Hyd Reflected Light Detectors (Hyd Rlds)

    Switching On the System Change password After logging on with your own user ID, you may change your password. To do so, press the Ctrl, Alt and Del keys at the same time. Then, click Change password. The Change password dialog box opens. Now you can start the LAS AF software.
  • Page 89 Switching On the System Status LED color Meaning Figure 75, item (see Green Operational readiness The maximum permitted signal level was exceeded. The detector was shutoff. Yellow Flashes if photons are being detected. Blue Cooling is active. • Never change or remove the filter cube or short pass filter during operation. •...
  • Page 90 Switching On the System...

  • Page 91: Las Af

    LAS AF LAS AF The LAS AF software is used to control all system functions and acts as the link to the individual hardware components. Image acquisition, image analysis and image processing are carried out using LAS AF. 13.1 Starting LAS AF NOTICE Damage is possible when contacting the specimen stage When using an inverted microscope, the illuminator arm must be swung...

  • Page 92: Structure Of The Graphical User Interface

    LAS AF 13.2 Structure of the Graphical User Interface Figure 77: Structure of the Graphical User Interface 1 Scaling range 7 Image display 2 Operating mode selection 7a Image display settings 3 Steps 7b Image processing and image analysis options 4 Menu 7c Channel display 5 Tab area...
  • Page 93 LAS AF • Acquire step: Experiments tab: Directory tree for opened files Acquisition tab: Hardware settings for the current experiment and parameter settings for image acquisition • Process step: Experiments tab: Directory tree for opened files Process Tools tab: Overview of the functions available in this step •...
  • Page 94 LAS AF Image acquisition buttons: These buttons are available to you during the Acquire and Quantify steps. Here you can select how the acquisition is to be started: • Autofocus: Focusing using the selected settings • Live: Acquisition of a live image •...

  • Page 95: Las Af Online Help

    LAS AF 13.3 LAS AF Online Help 13.3.1 Structure of Online Help Online Help is divided into seven main chapters: • General Information: Contains legal notices and general information on LAS AF. • About LAS AF Online Help: Contains general information for the LAS AF Online Help. •...

  • Page 96: Selecting The Language For Online Help

    LAS AF 13.3.3 Selecting the Language for Online Help By default, English is set as the language for Online Help. In order to select another language, follow these steps: 1. In LAS AF, switch to the Configuration step. 2. Click on the User Config button on the left side to access the User Configuration dialog. 3.

  • Page 97: Full-Text Search With Logically Connected Search Terms

    LAS AF • Search tab: Allows full-text searching. Enter the word you would like to search for in the search box. Click on the List Topics button. An alphabetically structured list of topics containing the keyword is displayed. 13.3.5 Full-text Search with Logically Connected Search Terms In Online Help, you can use the full-text search function to search for words and for phrases enclosed in quotation marks.
  • Page 98 LAS AF...

  • Page 99: Changing The Specimen

    Changing the Specimen Changing the Specimen WARNING Risk of permanent eye damage from laser radiation Never change specimens during the scanning operation because laser radiation can escape uncontrolled from the specimen area. 14.1 Changing the Specimen on an Upright Microscope To change specimens on an upright microscope, proceed as follows: 1.
  • Page 100 Changing the Specimen...

  • Page 101: Changing The Objective

    Changing the Objective Changing the Objective WARNING Risk of permanent eye and skin damage from laser radiation Never change objectives during the scanning operation because laser radiation can escape uncontrolled from the specimen area. To change objectives proceed as follows: 1.
  • Page 102 Changing the Objective...

  • Page 103: Piezo Focus On Upright Microscope

    • Do not make any adjustments to the piezo focus controller (see Figure 83), as it has already been optimally set up by Leica Service. • When replacing the objective on the piezo focus, you must perform a teach-in for the new objective in LAS.
  • Page 104 Piezo Focus on an Upright Microscope Figure 83: Piezo focus controller Figure 84: Spacer on objective...

  • Page 105: Switching Off The System

    Switching Off the System Switching Off the System The TCS SP8 and TCS SP8 MP systems are available with a flexible or compact supply unit. Observe the switch-off sequence that applies to your supply unit. NOTICE Damage to the instrument when not adhering to the switch-off sequence The switch-off sequence must be followed! When not adhering to the below listed switch-off sequence, the laser can be damaged.
  • Page 106 Switching Off the System 3. Turn off the lasers in the supply unit with the key switch on the main switch board of the flexible supply unit (see Figure 87, item 4). Figure 87: Overview of the main switch board on the flexible supply unit The emission warning indicator goes out.
  • Page 107 Switching Off the System Figure 89: Key Switch for the White Light Laser 6. If you are using external UV laser 355 including 405, press the key switch to turn it off. The emission warning indicators go out. Figure 90: Key switch on the power supply of external UV laser 355 Figure 91: Key switch on external UV laser 405...
  • Page 108 Switching Off the System Figure 92: Power switch on external UV laser 355 Figure 93: Power switch on external UV laser 405 8. If you are using a HyD RLD, switch it off at its supply unit: Figure 94: HyD RLD supply unit 9.
  • Page 109 Switching Off the System Figure 95: NDD detection unit 10. Switch off the microscope's control panel: Figure 96: Control panel for the microscope 11. Now, shut down the workstation. 12. Switch off the lasers (see Figure 87, item 3), the scan head (see Figure 87, item 2), the workstation and the microscope (see Figure 87, item 1) on the flexible supply unit's main switch board.

  • Page 110: Tcs Sp8 With Compact Supply Unit

    Switching Off the System 17.2 TCS SP8 with compact supply unit 1. Save the image data: Select the "Experiments" tab and click the Save all button. Figure 97: Saving the image data in LAS AF 2. Close the LAS AF software: On the menu bar, select File > Exit. Figure 98: Shutting down LAS AF 3.
  • Page 111 Switching Off the System The emission warning indicator goes out. 4. If you are using a HyD RLD, switch it off at its supply unit: Figure 100: HyD RLD supply unit 5. If your system has an infrared laser (MP) or an optical parametric oscillator (OPO), switch off the NDD detection unit: Figure 101: NDD detection unit...
  • Page 112 Switching Off the System item 2). The readiness indicator (Figure 103, item 1) on the electronics box goes out. Figure 103: Microscope electronics box 10. Turn off any accessories being used. 11. If you are using external UV laser 355 including 405, press the key switch to turn it off. The emission warning indicators go out.
  • Page 113 Switching Off the System Figure 106: Power switch on external UV laser 355 Figure 107: Power switch on external UV laser 405 Observe the user manuals for external lasers Please refer to the information from the documents provided by the laser manufacturer for the external lasers.
  • Page 114 Switching Off the System...

  • Page 115: Care And Cleaning

    Care and Cleaning Care and Cleaning Observe the user manuals provided Always observe all of the operating manuals provided for the individual components and peripheral devices. WARNING Electric shock Before cleaning, disconnect the entire system from the power supply. To do so, use the power switches of all components and disconnect all power cables from the power supply.

  • Page 116: Cleaning Immersion Lenses

    Caution when objective lens is contaminated If an objective lens is contaminated by unsuitable immersion oil or by the specimen, please contact your local Leica branch office. Certain solvents may dissolve the glue which holds the lens in place. 18.4 Care •...

  • Page 117: Repairs And Service Work

    • Be sure to back up your data before any service or repair work is performed. Leica Microsystems CMS GmbH shall not be liable for any loss of data.
  • Page 118 Repairs and Service Work...

  • Page 119: Maintenance

    You can find the safety data sheet for the coolant in the Chapter "Appendix". Maintenance and replacement of the coolant may only be carried out by service technicians who are authorized by Leica Microsystems CMS GmbH. Opening or working on the system in any way shall void any and all warranty claims.
  • Page 120 Maintenance...

  • Page 121: Disassembly And Transport

    Contact the Leica branch office in your country or your contact person if you need to move or transport the system or need to ship parts of it.
  • Page 122 Repairs and Service Work...

  • Page 123: Disposal

    Disposal Disposal At the end of the product service life, please contact the Leica branch office in your country with regard to disposal. Disposal The system, its accessory components and consumable materials must not be disposed of together with general household waste! Be sure to...
  • Page 124 Disposal...

  • Page 125: Contact

    Contact Contact If you have any further questions, please directly contact your country's Leica branch office or your local contact person. The appropriate contacts can be found on the Internet under: http://www.confocal-microscopy.com...
  • Page 126 Contact...

  • Page 127: Appendix

    6,678,443; 6,687,035; 6,738,190; 6,754,003; 6,771,405; 6,801,359; 6,831,780; 6,850,358; 6,852,964; 6,867,899; 7,016,101. Further patents are pending. The Leica TCS SP8 X product is protected by US patents: 5,886,784; 5,903,688; 6,137,627; 6,222,961; 6,285,019; 6,311,574; 6,355,919; 6,423,960; 6,433,814; 6,444,971; 6,466,381; 6,510,001; 6,611,643; 6,614,031; 6,614,525; 6,614,526; 6,654,165; 6,657,187;...
  • Page 128 Appendix EEC - SAFETY DATA SHEET Gem. 91/155/EG; 2001/58/EG innovatek OS GmbH Dated: www.innovatek.de February 28st 2011 / innovatek Protect application mixture info@innovatek.de 1. Substance/preparation and company name Trade name: innovatekProtect IP – application mixture company: innovatek OS GmbH, Stadtweg 9, 85134 Stammham Tel: 08405/92590 Fax: 08405/925921 Emergency phone No.: +49 (0) 8405/92590...
  • Page 129 Appendix EEC - SAFETY DATA SHEET Gem. 91/155/EG; 2001/58/EG innovatek OS GmbH Dated: www.innovatek.de February 28st 2011 / innovatek Protect application mixture info@innovatek.de 6. Accidental release measures Personal precautions: Avoid excessive contact with skin and eyes. In case of release of larger amounts remove contaminated clothing and wash bo- dy down thoroughly with water.
  • Page 130 Appendix EEC - SAFETY DATA SHEET Gem. 91/155/EG; 2001/58/EG innovatek OS GmbH Dated: www.innovatek.de February 28st 2011 / innovatek Protect application mixture info@innovatek.de Upper explosion limit: 15.0 % vol (only valid für the Ethylenglykol – part) Ignition point: >200°C (DIN 51794)(only valid für the Ethylenglykol – part) Vapor pressure (20°C): 2 mbar (DIN 51757) Density (20°C):...
  • Page 131 Appendix EEC - SAFETY DATA SHEET Gem. 91/155/EG; 2001/58/EG innovatek OS GmbH Dated: www.innovatek.de February 28st 2011 / innovatek Protect application mixture info@innovatek.de tural waters. The product has not been tested. The statement was derived from the properties of the individual components. 13.
  • Page 132 Appendix EEC - SAFETY DATA SHEET Gem. 91/155/EG; 2001/58/EG innovatek OS GmbH Dated: www.innovatek.de February 28st 2011 / innovatek Protect application mixture info@innovatek.de sponsible and liable by reason of intent or gross negligence. No liability will be accepted for damage indirectly incured. We provide this information and data according to our present level of knowledge and expe- rience.

  • Page 133: Compliance

    Appendix 24.3 Compliance This system has been tested and meets the requirements of the following standards: IEC/EN 61010-1:2011 "Safety requirements for electrical equipment for measurement, control and laboratory use - Part 1: General requirements" IEC/EN 60825-1:2007 "Safety of laser products - Part 1: Equipment classifications and requirements"...

  • Page 134: People's Republic Of China

    Appendix 24.4 People's Republic of China - Administrative Measures on the Control of Pollution Caused by Electronic Information Products - printed circuit boards electronic components mechanical parts optical components cables light sources Note: The actual product may or may not include all the part types listed above.
  • Page 136 Fax: +49 621 7028 - 1028 D-68165 Mannheim (Germany) http://www.leica-microsystems.com Copyright © Leica Microsystems CMS GmbH • All rights reserved User manual Leica TCS SP8 | Leica TCS SP8 MP | Leica TCS SP8 X Order No.: 158000002 | V: 00...

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