Leica TCS SP8 SMD User Manual page 128

For fcs, flim and flcs
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FLIM Data Acquisition
Figure 111:
2. In Beam Path Settings, enable the desired photomultiplier by clicking the corresponding
button (Figure 112, item 1).
3. When a detector is activated, it can be recognized by a shadow with a slider below the
electromagnetic spectrum. This slider adjusts the wavelength range detected by the
detector (Figure 112, item 2).
Figure 112:
4. If the system has SP FLIM detectors, the FLIM option is displayed for the respective
detector in Beam Path Settings and you can use SP FLIM detectors for the image
acquisition.
When you activate FLIM (switch is at ON – Figure 113, item 1), the Gain of the detectors
is automatically set to a value of 990 V.
When switched to OFF (Figure 113, item 2), the detector can be used for normal image
acquisition. You can also adjust the gain of the detectors as usual.
Figure 113:
5. For multi-channel image acquisition you can use the default image acquisition PMTs
(Figure 114, item 1) along with the SP FLIM photomultipliers (Figure 114, item 2).
128
Selecting the Mirror setting for X1 Port
Selecting detector and wavelength range in Beam Path Settings
Selecting the operating mode of the internal SP FLIM detectors in Beam Path Settings

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