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6. Via the λ stack dialog, define the stack as described in Chapter 16.3.7 (Figure 142).
7. Define the duration of the individual FLIM images as described in Chapter 16.3.3. Use
either Duration of each image or Repetitions, because the last option, Acquire until max
___ photons/pixel, does not enable the specification of a fixed period of time.
8. You can define a time series using the time series dialog (Figure 143). The following
inputs are possible:
• Time Interval: Defines the duration between the beginning of an FLIM stack and the
next stack. This time span cannot be shorter than the timespan required for an
λ
individual FLIM stack. To specify the smallest possible duration, select Minimize.
• Duration: Here, enter how long the entire time series is to last. The corresponding
λ
number of FLIM stacks is acquired. The entered duration is automatically corrected
λ
so that the last FLIM stack can be completed.
• Stacks: Here, you can specify the number of
maximum specified number of FLIM images is 1000.
Figure 143:
Defining a time series of xz
λ
FLIM stacks to be acquired. The
λ
FLIM stacks
FLIM Data Acquisition
λ
159
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