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Figure 152:
IRF oscilloscope window, acquired with PH300, APD of type MPD and PQ laser source
9. The time width per channel can be set with the Resol. slider. After a change, select the
Apply button.
10. Quit the oscilloscope display in SymPhoTime by selecting the Stop button.
Steps 7 and 10 are used only to check the IRF shape; they do not have to be carried out
routinely.
11. End the scanning operation in test mode in LAS AF by selecting Stop FLIM Test again.
12. Go to the Measurements step in the FLIM Wizard.
13. Start the scan procedure in LAS AF by selecting the Run FLIM image acquisition button.
Normally, the SymPhoTime software will start the data acquisition automatically. You
can also start the FLIM data acquisition by selecting the Acquire LSM Measurements
button. In both cases, data are saved.
14. Quit the image acquisition in LAS AF by clicking on Stop FLIM.
15. Replace the IRF specimen with your specimen and reinsert the correct filter for your
specimen. Open the TCSPC histogram of the previously acquired image. It contains the
IRF.
Summarized manual for FLIM experiments
175
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