Leica TCS SP8 STED 3X User Manual
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Leica TCS SP8 STED 3X User Manual

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From Eye to Insight
User Manual
Leica TCS SP8 STED 3X
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  • Page 1 From Eye to Insight User Manual Leica TCS SP8 STED 3X Leica TCS SP8 STED-ONE...
  • Page 2 Published by: Leica Microsystems CMS GmbH Am Friedensplatz 3 D-68165 Mannheim (Germany) http://www.leica-microsystems.com http://www.confocal-microscopy.com Responsible for contents: Leica Microsystems CMS GmbH Copyright © Leica Microsystems CMS GmbH. All rights reserved.
  • Page 3 Copyright Copyright All rights to this document are held by Leica Microsystems CMS GmbH. Adaptation, translation and reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica Microsystems CMS GmbH.
  • Page 4 Copyright...

  • Page 5: Table Of Contents

    Contents Contents 1 About this User Manual ............9 2 Intended Use .
  • Page 6 Contents 8 Ambient Conditions............33 General Requirements Regarding Ambient Conditions.
  • Page 7 Contents 10.5.3 Manual Laser Interlock Switch ........50 10.5.4 Interlock Connector on the White Light Laser .
  • Page 8 Contents 13.4.2 Selecting the language for Online Help ....... . .86 13.4.3 Using Online Help.

  • Page 9: About This User Manual

    If you have any suggestions or improvements for this User Manual, please contact the Leica branch office in your country.
  • Page 10 About this User Manual...

  • Page 11: Intended Use

    The manufacturer assumes no liability for damage caused by, or any risks arising from, use of the microscopes for purposes other than those for which they are intended, or not using the microscopes within the specifications of Leica Microsystems CMS GmbH. In such cases, the Declaration of Conformity shall be invalid.
  • Page 12 Intended Use...

  • Page 13: Liability And Warranty

    Figures are for illustration purposes. The system you purchased can deviate from the illustrations without Leica Microsystems CMS GmbH explicitly specifying such. Leica Microsystems CMS GmbH shall not be liable for any injury or property damage caused by untrained or unauthorized persons.
  • Page 14 Liability and Warranty...

  • Page 15: Meaning Of The Warning Messages In The Manual

    Meaning of the warning messages in the manual Meaning of the warning messages in the manual WARNING Electric shock This warns you of hazardous electrical voltage. Following the instructions is mandatory, since otherwise there is a risk of severe or fatal injury.
  • Page 16 Meaning of the warning messages in the manual CAUTION Injuries from… This note warns you of minor to moderate injuries that can be prevented by following instructions. NOTICE Risk of damage to the system This note describes possible material damage that can occur in case of misuse.

  • Page 17: General Safety Notes

    Commissioning and use • The system is delivered in secure transport packaging. Only Leica service technicians or people authorized by Leica Microsystems CMS GmbH may open the transport packaging and unpack and assemble the system.

  • Page 18: Electrical Safety

    • Before any cleaning or servicing, de-energize the entire system. To do so, use the power switches of all components and disconnect all power cables from the power supply. • Only use the power cables included or provided by your local Leica service technicians for connecting individual peripheral devices to the power supply.

  • Page 19: Contact With Liquids

    Figure 1: Specimen area of upright and inverted microscope • Only use S1 and S28 Leica microscope condensers. • Do not use an S70 microscope condenser. Its large working distance and lower numerical aperture could pose a hazard due to laser radiation.

  • Page 20: Modifications To The System

    • The emission warning indicator is re-lit after being switched off using the key switch. • Scanning of the specimen is not activated after being switched on properly (laser radiation in the specimen area). If any of these occur, immediately notify the Leica branch office in your country or your local contact person.

  • Page 21: Additional Notes On Handling The System

    • We recommend using a room that can be completely darkened. • Do not expose the system to drafts. • If the system has to be moved to a new location for any reason, contact the Leica branch office in your country.

  • Page 22: Protecting The System

    If the maximum permitted signal level is exceeded, the detector automatically switches off and the red status LED on the detector module lights • If you have any further questions, please directly contact the Leica branch office in your country (see Chapter "Contact").

  • Page 23: Objectives

    Additional Notes on Handling the System 6.3.1 Objectives • Only use immersion fluids that are intended for the objective. Unsuitable immersion fluid can contaminate or destroy the objective. • When changing over from an oil or water objective to a dry objective, you have to remove the immersion medium from the specimen slide in order not to damage the dry objective.
  • Page 24 Additional Notes on Handling the System...

  • Page 25: System Overview And Properties

    System Overview and Properties System Overview and Properties Figures are for illustration purposes. The system you purchased can deviate from the illustrations without Leica Microsystems CMS GmbH explicitly specifying such. System variants 7.1.1 System with Inverted Microscope and Flexible Supply Unit...

  • Page 26: System With Inverted Microscope And Compact Supply Unit

    System Overview and Properties 7.1.2 System with Inverted Microscope and Compact Supply Unit Figure 3: System overview and dimensions 1 STED module 7 Workstation 2 Scan head 8 Inverted microscope 3 SmartMove 9 Compact light source 4 Compact supply unit 10 Optical table 5 Main switch board 11 19‘‘...

  • Page 27: System With Upright Microscope And Flexible Supply Unit

    System Overview and Properties 7.1.3 System with Upright Microscope and Flexible Supply Unit Figure 4: System overview and dimensions 1 White light laser 7 SmartMove 2 STED module 8 Microscope electronics box 3 Scan head 9 Workstation 4 Main switch board 10 Compact light source 5 Flexible supply unit 11 Upright microscope...

  • Page 28: System With Upright Microscope And Compact Supply Unit

    System Overview and Properties 7.1.4 System with Upright Microscope and Compact Supply Unit Figure 5: System overview and dimensions 1 STED module 7 Workstation 2 Scan head 8 Compact light source 3 SmartMove 9 Upright microscope 4 Compact supply unit 10 Optical table 5 Main switch board 11 19‘‘...

  • Page 29: Controls On The Supply Unit

    System Overview and Properties Controls on the Supply Unit 7.2.1 Main Switch Board on the Flexible Supply Unit Figure 6: Overview of the main switch board on the supply unit 7.2.2 Control Panel Field on the Compact Supply Unit Figure 7: Overview of the control panel field on the compact supply unit...

  • Page 30: Technical Data

    System Overview and Properties Technical Data 7.3.1 Dimensions Dimensions of the system (length x depth x height) 320 x 150 x 140 cm (10'6" x 4'11" x 4'7") 7.3.2 Weight of the supply unit Flexible supply unit 90 kg (198 lbs) Compact supply unit 40 kg (88 lbs) 7.3.3...

  • Page 31: Electromagnetic Compatibility

    System Overview and Properties "Electromagnetic Compatibility" With regard to emitted interference this concerns a Class A device (CISPR 11). This device is designed for use in buildings that do not include domestic premises and buildings not directly connected to a low-voltage power supply network that supplies buildings used for domestic purposes.
  • Page 32 System Overview and Properties...

  • Page 33: Ambient Conditions

    Otherwise, this could cause fire and electrical shocks to the system and the electrical components. • The room should be equipped with a telephone connection to contact Leica Microsystems CMS GmbH for phone support. For RemoteCare, an Internet connection is also needed.

  • Page 34: Vibrations

    Ambient Conditions Vibrations Vibrations must be reduced to a minimum; for that reason, do not set up the system near any of the following sources of vibration: shakers, ultracentrifuges, pumps, compressors, refrigerators, vending machines, elevators. Maximum tolerable vibrations: Frequency range [5 Hz – 30 Hz] less than 12,5 μm/s root mean square Frequency range [>...
  • Page 35 Ambient Conditions Power connection Four separate electric circuits with Power supply voltage: 100 V - 120 V, fuse protection: 20 A Power supply voltage: 200 V - 240 V, fuse protection: 12 - 16 A (Two for the supply unit's power supply and two for supplying peripheral devices).

  • Page 36: System With Compact Supply Unit

    Ambient Conditions 8.4.2 System with compact supply unit Supply voltage 100 V~ to 240 V~ ± 10%, grounded Frequency 50/60 Hz Power connection Three separate electric circuits with Power supply voltage: 100 V - 120 V, fuse protection: 20 A Power supply voltage: 200 V - 240 V, fuse protection: 12 - 16 A (One for the compact supply unit's power supply and one for supplying peripheral devices)

  • Page 37: Load Capacity Of The Multiple Socket Outlet On The Flexible Supply Unit

    Ambient Conditions Load capacity of the multiple socket outlet on the flexible supply unit The total power consumption of all loads connected to the multiple socket outlet (see Figure 9) must not exceed 800 VA. The terminals are intended for: •...

  • Page 38: System With Flexible Supply Unit

    Ambient Conditions 8.6.1 System with flexible supply unit Waste heat of the complete 4,0 kW system including the argon laser Waste heat of the argon laser 1.6 kW For cooling, the argon laser must be connected to the provided argon laser fan. The following space is required: Standard fan about 30 x 30 cm (1' x 1')

  • Page 39: Laser

    Laser Laser It is mandatory to observe the laser safety measures for laser class 3B / IIIb (VIS and UV lasers) or for laser class 4 / IV (MP systems) in accordance with applicable national and federal regulations. You may only use the laser listed here, as the laser safety devices are only designed for the laser variants listed here.

  • Page 40: Ir Lasers For Sted System

    Laser DPSS 561 < 100 < 12 Continuous wave (cw) HeNe < 4 < 1 Continuous wave (cw) HeNe < 15 < 5 Continuous wave (cw) Diode 635 Pulsed, 60 ps Diode 638 Continuous wave (cw) 9.2.2 IR Lasers for STED System The listed lasers (see Chapter 9.2.2.1 and Chapter 9.2.2.2) can be used for the STED system for multiphoton excitation in confocal operation.

  • Page 41: Depletion Laser For Tcs Sp8 Sted System

    Laser Chameleon Vision II 680 - 1080 < 4.0 < 1.9 Pulsed, ~ 140 fs Compact OPO 1000-1280 < 1.6 < 0.8 Pulsed, > 100 fs 9.2.3 Depletion Laser for TCS SP8 STED System Laser type Wavelength Maximum Maximum Pulse duration (nm) light output at light output in...
  • Page 42 Laser...

  • Page 43: 10 Safety Features

    The light path remains interrupted until all doors have been closed. The viewing window (see Figure 10, item 1) offers protection from the scattered radiation of the infrared lasers provided by Leica Microsystems CMS GmbH. Laser Safety Chamber Figure 10: 10.2...

  • Page 44: Key Switch

    Safety Features The supply unit must be set up so that the main circuit breaker is freely accessible at all times. Figure 11: Rear side of the flexible supply unit with the main circuit breaker 10.3 Key Switch 10.3.1 Master Key Switch on the Compact Supply Unit There is a master key switch on the compact supply unit for protection against unauthorized use of the laser products.

  • Page 45: Key Switch For The White Light Laser

    Safety Features The master key switch for the flexible supply unit is located on the front side of the main switch board (see Figure 13). Figure 13: Master key switch on the main switch board of the flexible supply unit 10.3.3 Key Switch for the White Light Laser The external lasers can also be turned off individually.

  • Page 46: Emission Warning Indicators

    Safety Features 10.4 Emission Warning Indicators The operational readiness of lasers is signaled by an emission warning indicator. WARNING Possible risk of permanent eye and skin damage from laser radiation in the specimen plane As soon as the emission warning indicator of the lasers is lit, it is possible from a functional standpoint that laser radiation is present in the specimen area.

  • Page 47: Emission Warning Indicator On The White Light Laser

    • Scanning of the specimen is not activated after being switched on properly (laser radiation in the specimen area). If any of these occur, immediately notify the Leica branch office in your country or your local contact person.

  • Page 48: Interlock Connectors

    Safety Features 10.5 Interlock Connectors 10.5.1 Interlock Connector on the Supply Unit The interlock connector (plug connection for the remote-controlled safety "interlock") is on the rear side of the supply unit. Operating voltage is 12 V DC (for the flexible supply unit see Figure 19; for the compact supply unit see Figure 18).

  • Page 49: Interlock Connector On The Laser Safety Chamber

    Safety Features Figure 19: Interlock connector on the rear side of the flexible supply unit 10.5.2 Interlock Connector on the Laser Safety Chamber Connections on the right side of the laser safety chamber Figure 20: 1 Interlock connector 2 Power supply connection for internal illumination of the laser safety chamber...

  • Page 50: Manual Laser Interlock Switch

    Safety Features 10.5.3 Manual Laser Interlock Switch The system features a manual laser interlock switch (see Figure 21) that is connected directly to the supply unit. The interlock connector of the laser safety chambers is connected to the manual laser interlock switch.

  • Page 51: Interlock Connector On Other External Lasers

    Safety Features Interlock connector when using the white light laser If the white light laser is operated as a component of the TCS SP8 X system, you have to use the interlock connector on the supply unit! The shorting plug must be connected to the interlock connector of the white light laser.

  • Page 52: Safety Switches On The Microscope

    Safety Features 10.6 Safety Switches on the Microscope When the safety switches are triggered, the light path of the laser beam is interrupted. Figure 24: Safety switches (1) and (2) on the inverted microscope; Position of Is triggered Is triggered Function the safety switch...

  • Page 53: Warning Messages

    LAS X on the functioning properly closed. No further use of the laser is monitor possible. In this situation, the system may not be operated. Contact the Leica branch office in your country or your contact person. Time-based Defective part in the shutter...

  • Page 54: Special Laser Safety Equipment

    Safety Features 10.8 Special Laser Safety Equipment 10.8.1 Laser Protection Tube and Laser Protection Shield The laser protection tube and the laser protection shield are used in inverted microscopes for protection from laser radiation (see Figure 25). Never remove the laser protection shield, laser protection tube or other laser protection equipment on the system.

  • Page 55: Safety Beam Guide

    Safety Features 10.8.2 Safety beam guide The light of all employed VIS lasers (wavelength range 400 - 700 nm, visible spectrum) and STED lasers is fed through a fiber optic cable and, therefore, completely shielded until it leaves the microscope objective and reaches the specimen. For systems with infrared laser (wavelength range >...
  • Page 56 Safety Features...

  • Page 57: Safety Labels On The System

    Missing or damaged safety labels must be attached immediately and at the described location. Operation without the safety labels shown is not permitted. If you have any further questions, contact your laser safety officer or the Leica branch office in your country immediately.

  • Page 58: Safety Labels On The Laser Safety Chamber

    Safety Labels on the System 11.1 Safety labels on the Laser Safety Chamber Figure 27: Safety labels on the laser safety chamber Position System Safety label in English Safety label in German DANGER VORSICHT VISIBLE AND INVISIBLE LASER SICHTBARE UND UNSICHTBARE RADIATION CLASS 4 LASERSTRAHLUNG KLASSE 4 WHEN OPEN AVOID EYE OR SKIN...

  • Page 59: Inverted Microscope

    Safety Labels on the System 11.2 Inverted Microscope Figure 28: Safety label for the inverted microscope Position System Safety label in English Safety label in German VISIBLE AND INVISIBLE LASER AUSTRITT VON SICHTBARER RADIATION IS EMITTED FROM UND UNSICHTBARER THIS APERTURE LASERSTRAHLUNG AVOID EXPOSURE BESTRAHLUNG VERMEIDEN...

  • Page 60: Inverted Microscope With Dls

    Safety Labels on the System 11.2.1 Inverted Microscope with DLS Figure 29: Safety label for the inverted microscope with DLS 11.3 Upright Microscope Rear view of microscope: Figure 30: Safety label for the upright microscope...
  • Page 61 Safety Labels on the System Position System Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE WENN ABDECKUNG GEÖFFNET TO BEAM NICHT DEM STRAHL AUSSETZEN VORSICHT DANGER SICHTBARE UND UNSICHTBARE VISIBLE AND INVISIBLE LASER LASERSTRAHLUNG KLASSE 3B RADIATION CLASS 3B...

  • Page 62: Scan Head

    Safety Labels on the System Position System Safety label in English Safety label in German LASER RADIATION IS EMITTED AUSTRITT VON FROM THIS APERTURE LASERSTRAHLUNG AVOID EXPOSURE BESTRAHLUNG VERMEIDEN 1 and 2 UV/MP VISIBLE AND INVISIBLE LASER AUSTRITT VON SICHTBARER RADIATION IS EMITTED FROM UND UNSICHTBARER THIS APERTURE...
  • Page 63 Safety Labels on the System Position Safety label in English Safety label in German DANGER VORSICHT VISIBLE AND INVISIBLE LASER SICHTBARE UND UNSICHTBARE RADIATION CLASS 4 LASERSTRAHLUNG KLASSE 4 WHEN OPEN AVOID EYE OR SKIN WENN ABDECKUNG GEÖFFNET EXPOSURE TO DIRECT OR BESTRAHLUNG VON AUGE ODER SCATTERED RADIATION HAUT DURCH DIREKTE ODER...

  • Page 64: Sted Module

    Safety Labels on the System 11.5 STED Module Figure 33: Safety label on the STED module Position Safety label in English Safety label in German DANGER VORSICHT VISIBLE AND INVISIBLE LASER SICHTBARE UND UNSICHTBARE RADIATION CLASS 4 LASERSTRAHLUNG KLASSE 4 WHEN OPEN AVOID EYE OR SKIN WENN ABDECKUNG GEÖFFNET EXPOSURE TO DIRECT OR...

  • Page 65: Compact Supply Unit

    Safety Labels on the System 11.6 Compact Supply Unit View of the compact supply unit: Figure 34: Safety labels on the compact supply unit Position Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE TO WENN ABDECKUNG GEÖFFNET NICHT BEAM...

  • Page 66: Flexible Supply Unit

    Safety Labels on the System 11.7 Flexible Supply Unit View of the flexible supply unit: Figure 35: Safety labels on the flexible supply unit Position Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE TO WENN ABDECKUNG GEÖFFNET NICHT BEAM...

  • Page 67: Beam Coupling Unit

    Safety Labels on the System 11.8 Beam Coupling Unit View of the beam coupling unit: Figure 36: Safety label for the beam coupling unit (top side) Position Safety label in English Safety label in German DANGER VORSICHT VISIBLE AND INVISIBLE LASER SICHTBARE UND UNSICHTBARE RADIATION CLASS 4 LASERSTRAHLUNG KLASSE 4...

  • Page 68: Cover For Replacement Flange

    Safety Labels on the System 11.9 Cover for Replacement Flange Figure 37: Cover for replacement flange at different microscopes Front view of the cover: Figure 38: Safety label on the cover Position Safety label in English Safety label in German DANGER VORSICHT VISIBLE AND INVISIBLE LASER...

  • Page 69: Hyd Rld

    Safety Labels on the System 11.10 HyD RLD Figure 39: Safety label on the HyD RLD Position Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE TO WENN ABDECKUNG GEÖFFNET NICHT BEAM DEM STRAHL AUSSETZEN Table 11:...
  • Page 70 Safety Labels on the System Position Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE TO WENN ABDECKUNG GEÖFFNET NICHT BEAM DEM STRAHL AUSSETZEN LASER RADIATION LASERSTRAHLUNG AVOID DIRECT EXPOSURE TO BEAM NICHT DEM STRAHL AUSSETZEN <...

  • Page 71: Switching The System On/Off

    Switching the System On/Off Switching the System On/Off The system comes with a flexible or compact supply unit. Observe the switch on/off sequence that applies to your supply unit. NOTICE Damage is possible when contacting the specimen stage When using an inverted microscope, the illuminator arm must be swung back before the system start and LAS X start because the stage can damage the condenser, the objective or the specimen during initialization.
  • Page 72 Switching the System On/Off Switch on the workstation and the microscope on the main switch board of the flexible supply unit. The operating system starts automatically when you switch on the workstation. Wait until the startup process has concluded. If the microscope's readiness indicator is not lit, activate the toggle switch on the electronics box.
  • Page 73 Switching the System On/Off To switch on the white light laser, activate the key switch on the front side of the white light laser. White light laser error messages An error report appears on the display of the white light laser: •...

  • Page 74: Switch-Off Sequence

    Switching the System On/Off 12.1.2 Switch-off Sequence NOTICE Damage to the instrument when not adhering to the switch-off sequence The switch-off sequence must be followed! When not adhering to the below listed switch-off sequence, the laser can be damaged. Observe the user manuals for external lasers Please refer to the information from the documents provided by the laser manufacturer for the external lasers.
  • Page 75 Switching the System On/Off If your system has an infrared laser (MP) or an optical parametric oscillator (OPO), switch off the NDD detection unit. Now, shut down the workstation. Switch off the lasers on the main switch board of the flexible supply unit.

  • Page 76: System With Compact Supply Unit

    Switching the System On/Off 12.2 System with Compact Supply Unit The sequence below describes how the components of the system must be switched on/off with a compact supply unit. 12.2.1 Switch-on Sequence Observe the user manuals for external lasers Please refer to the information from the documents provided by the laser manufacturer for the external lasers.
  • Page 77 Switching the System On/Off IR components If your system has an infrared laser (MP) or an optical parametric oscillator (OPO), switch on these components as described in the manufacturer's respective user manual. To switch on the lasers in the supply unit, use the key switch on the front side of the compact supply unit.

  • Page 78: Switch-Off Sequence

    Switching the System On/Off 12.2.2 Switch-off Sequence NOTICE Damage to the instrument when not adhering to the switch-off sequence The switch-off sequence must be followed! When not adhering to the below listed switch-off sequence, the laser can be damaged. Observe the user manuals for external lasers Please refer to the information from the documents provided by the laser manufacturer for the external lasers.
  • Page 79 Switching the System On/Off Switch off the scan head on the front side of the compact supply unit. Turn off the microscope by actuating the toggle switch on the electronics box. The readiness indicator on the electronics box goes out. Turn off any accessories being used.

  • Page 80: Hyd Reflected Light Detectors (Hyd Rlds)

    Switching the System On/Off 12.3 HyD reflected light detectors (HyD RLDs) If you are using HyD RLDs you have to switch them on before LAS X starts. Otherwise, the LAS X software cannot initialize the detectors. The HyD RLDs consist of a supply unit (see Figure 41) and a detector module (see Figure 42).

  • Page 81: Starting Las X

    LAS X LAS X The LAS X software is used to control all system functions and acts as the link to the individual hardware components. Image acquisition, image analysis and image processing are carried out using LAS X. 13.1 Starting LAS X NOTICE Damage is possible when contacting the specimen stage When using an inverted microscope, the illuminator arm must be swung...

  • Page 82: Structure Of The Graphical User Interface

    LAS X 13.2 Structure of the Graphical User Interface Figure 44: Structure of the Graphical User Interface 1 Scaling range 7 Display window 2 Operating mode selection 7a Display window settings 3 Steps 7b Image processing and image analysis options 4 Menu 7c Channel display 5 Tab area...
  • Page 83 LAS X Tab area: The displayed tabs depend on the selected step. In the TCS SP8's basic operating mode, the following tabs are assigned to the steps: • Acquire step:  Project tab: Directory tree for opened files Acquisition tab: Hardware settings for the current experiment and parameter settings for image acquisition •...
  • Page 84 LAS X Channel display: Here, you can select how the acquired image is to be displayed. If you have excited your specimen with multiple laser lines, you can use the buttons to select whether the different channels are displayed individually, next to each other or in one image. Image acquisition buttons:...

  • Page 85: Activating/Deactivating The Sted In Las X

    LAS X experiment by right-clicking it: Figure 46: Possible export formats 13.3 Activating/Deactivating the STED in LAS X In LAS X, activate the STED laser using the Activate STED button, see Figure 47. Figure 47: Activating STED in LAS X...

  • Page 86: Las X Online Help

    LAS X In LAS X, deactivate the STED laser using the Deactivate STED button, see Figure 48. Figure 48: Deactivating STED in LAS X 13.4 LAS X Online Help 13.4.1 Accessing Online Help In the menu bar, click on the Help menu. The menu drops down and reveals search-related options, including the following: Figure 49: Online Help menu...

  • Page 87: Using Online Help

    LAS X Figure 50: Selecting the language for Online Help The next time you call up Online Help, the help topics will be displayed in the selected language. The Help menu provides a second option for changing the language. Call up the Help -> Options menu.

  • Page 88: Searching In Online Help

    LAS X 13.4.4 Searching in Online Help You can use the search field to search for specific terms in Online Help (see Figure 51). Online Help displays a hit list for you. Figure 51: The search field in Online Help...

  • Page 89: Changing The Specimen

    Changing the Specimen Changing the Specimen WARNING Risk of permanent eye damage from laser radiation Never change specimens during the scanning operation because laser radiation can escape uncontrolled from the specimen area. 14.1 Changing the Specimen on an Upright Microscope To change specimens on an upright microscope, proceed as follows: 1.
  • Page 90 Changing the Specimen...

  • Page 91: Changing The Objective

    Changing the Objective Changing the Objective WARNING Risk of permanent eye and skin damage from laser radiation Never change objectives during the scanning operation because laser radiation can escape uncontrolled from the specimen area. To change objectives proceed as follows: 1.
  • Page 92 Changing the Objective...

  • Page 93: Lambda/4 Plate Holder

    For optimum STED performance, the use of a /4 plate holder in the analyzer slot of the Leica DMI6000 microscope is absolutely necessary, see Figure 52. • When pulling out and inserting the /4 plate holder, ensure that the /4 plate is not scratched or soiled.
  • Page 94 Lambda/4 Plate Holder 4. Open the fluorescence module drawer. 5. Pull out the fluorescence module. 6. Remove the filter cube/beam splitter. 7. Insert the desired filter cube/beam splitter. 8. Reinsert the fluorescence module. 9. Close the fluorescence module drawer. 10. Carefully insert the /4 plate holder in the analyzer slot, see Figure 52.

  • Page 95: Care And Cleaning

    Microscope-internal optical components and filter cubes must only be cleaned by service technicians authorized by Leica Microsystems CMS GmbH. If damage appears on inner surfaces, the optical components must be sent to your Leica subsidiary for repair. • Never open the objectives for cleaning.

  • Page 96: Cleaning The Front Lens Of Immersion Objectives

    Caution when objective lens is contaminated If an objective lens is contaminated by unsuitable immersion oil or by the specimen, please contact your local Leica branch office. 17.3 Care • Always keep the optical components of the microscope clean.

  • Page 97: Repairs And Service Work

    • Be sure to back up your data before any service or repair work is performed. Leica Microsystems CMS GmbH shall not be liable for any loss of data.
  • Page 98 Repairs and Service Work...

  • Page 99: Maintenance

    You can find the safety data sheet for the coolant in the Chapter "Appendix". Maintenance and replacement of the coolant may only be carried out by service technicians who are authorized by Leica Microsystems CMS GmbH. Opening or working on the system in any way shall void any and all warranty claims.

  • Page 100: Refilling Coolant In The External Coolant Tank

    If air is sucked into the circuit, keep the system switched off and notify the Leica branch office in your country or your local contact person. NOTICE...

  • Page 101: Disassembly And Transport

    Contact the Leica branch office in your country or your contact person if you need to move or transport the system or need to ship parts of it.
  • Page 102 Disassembly and Transport...

  • Page 103: Disposal

    Disposal Disposal At the end of the product service life, please contact the Leica branch office in your country with regard to disposal. Disposal The system, its accessory components and consumable materials must not be disposed of together with general household waste! Be sure to...
  • Page 104 Disposal...

  • Page 105: Contact

    Contact Contact If you have any further questions or require technical support, please directly contact your country's Leica branch office or your local contact person. The appropriate contacts can be found on the Internet under: http://www.confocal-microscopy.com...
  • Page 106 Contact...

  • Page 107: Appendix

    Appendix Appendix 23.1 Patents The Leica TCS SP8 STED 3X and Leica TCS SP8 STED-ONE is protected by US patents: 5,731,588; 5,886,784; 5,903,688; 6,137,627; 6,222,961; 6,285,019; 6,311,574; 6,355,919;  6,423,960; 6,433,814; 6,444,971; 6,466,381; 6,510,001; 6,555,826; 6,614,526; 6,654,165;  6,657,187; 6,678,443; 6,687,035; 6,738,190; 6,754,003; 6,801,359; 6,813,073; 6,831,780;  6,850,358;  6,867,899; 6,914,236; 6,958,470; 6,975,394; 7,009,161, 7,599,115, 8,319,970. Further patents are pending. 23.2 Safety Data Sheets from Third-Party Manufacturers The scan head is liquid-cooled. Following are the safety data sheets from the manufacturer...
  • Page 108 Appendix EEC - SAFETY DATA SHEET Gem. 91/155/EG; 2001/58/EG innovatek OS GmbH Dated: www.innovatek.de February 28st 2011 / innovatek Protect application mixture info@innovatek.de 1. Substance/preparation and company name Trade name: innovatekProtect IP – application mixture company: innovatek OS GmbH, Stadtweg 9, 85134 Stammham Tel: 08405/92590 Fax: 08405/925921 Emergency phone No.: +49 (0) 8405/92590...
  • Page 109 Appendix EEC - SAFETY DATA SHEET Gem. 91/155/EG; 2001/58/EG innovatek OS GmbH Dated: www.innovatek.de February 28st 2011 / innovatek Protect application mixture info@innovatek.de 6. Accidental release measures Personal precautions: Avoid excessive contact with skin and eyes. In case of release of larger amounts remove contaminated clothing and wash bo- dy down thoroughly with water.
  • Page 110 Appendix EEC - SAFETY DATA SHEET Gem. 91/155/EG; 2001/58/EG innovatek OS GmbH Dated: www.innovatek.de February 28st 2011 / innovatek Protect application mixture info@innovatek.de Upper explosion limit: 15.0 % vol (only valid für the Ethylenglykol – part) Ignition point: >200°C (DIN 51794)(only valid für the Ethylenglykol – part) Vapor pressure (20°C): 2 mbar (DIN 51757) Density (20°C):...
  • Page 111 Appendix EEC - SAFETY DATA SHEET Gem. 91/155/EG; 2001/58/EG innovatek OS GmbH Dated: www.innovatek.de February 28st 2011 / innovatek Protect application mixture info@innovatek.de tural waters. The product has not been tested. The statement was derived from the properties of the individual components. 13.
  • Page 112 Appendix EEC - SAFETY DATA SHEET Gem. 91/155/EG; 2001/58/EG innovatek OS GmbH Dated: www.innovatek.de February 28st 2011 / innovatek Protect application mixture info@innovatek.de sponsible and liable by reason of intent or gross negligence. No liability will be accepted for damage indirectly incured. We provide this information and data according to our present level of knowledge and expe- rience.

  • Page 113: Compliance

    Appendix 23.3 Compliance This system has been tested and meets the requirements of the following standards: IEC/EN 61010-1:2011 "Safety requirements for electrical equipment for measurement, control and laboratory use - Part 1: General requirements" IEC/EN 60825-1:2007 "Safety of laser products - Part 1: Equipment classifications and requirements"...
  • Page 114 Appendix Figure 60: Declaration of Conformity...

  • Page 115: People's Republic Of China

    Appendix 23.4 People's Republic of China - Administrative Measures on the Control of Pollution Caused by Electronic Information Products - printed circuit boards electronic components mechanical parts optical components cables light sources Note: The actual product may or may not include all the part types listed above.
  • Page 116 Appendix...
  • Page 118 Fax: +49 621 7028 - 1028 D-68165 Mannheim (Germany) http://www.leica-microsystems.com Copyright © Leica Microsystems CMS GmbH • All rights reserved User Manual Leica TCS SP8 STED 3X | Leica TCS SP8 STED-ONE Ordernumber: 158000052 | V: 04 | June 08, 2017...

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