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F(L)CS Data Acquisition
Figure 160:
5. Move the focus using the z-drive of the microscope stand until you see the two reflection
lines that appear between the immersion and the glass and between the glass and the
specimen (Figure 161, only visible simultaneously at zoom 1).
Figure 161:
184
Optimization of the correction ring in the Setup Corr-ring step
Reflection at the transition between the cover slip and the immersion water (top) and
between the cover slip and the specimen (bottom) on the inverted stand. The objective is
optimized relative to the bottom reflection. (The top and bottom reflection are
simultaneously visible only for zoom 1. However, this can be set only if the objective cap
was previously disabled in LAS AF.)
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