Leica TCS SP8 SMD User Manual page 180

For fcs, flim and flcs
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F(L)CS Data Acquisition
select the Set Calibration button (Figure 156, item 2).
Figure 156:
The calibration data will be stored by the system and displayed under X and Y (Figure 157).
Figure 157:
180
Procedure for calibrating the FCS measurement position – Definition of the bleach point
Display of the calibration data
Optimized calibration
The calibration is optimized primarily with regard to the scan speed
defined during the calibration (default value: 400 Hz). The calibration
applies for any zoom.

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