Leica TCS SP8 SMD User Manual page 126

For fcs, flim and flcs
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Selecting the Laser
126
Automatic MFP positioning
Within the FLIM Wizard, the MFP positioning is done automatically. MFP
positioning occurs at the corresponding beam splitters whenever a laser
selection is changed. Outside of the wizard, the user must configure this
setting on his or her own.
Settings made automatically can be modified later by the user. These
user-defined settings can be saved and reloaded later.
Continuous wave mode (cw mode)
Depending on the configuration, the mode of the pulsed 405 nm laser can
be changed to continuous wave mode on the PDL800D or SEPIA II driver
(see corresponding manuals). In continuous wave mode, no beam
blanking of the laser is active. This means that creating a special region
of interest (ROI) and line-by-line sequential scanning do not work!

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