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In each step, two to three different tabs are shown in the tab area:
• On the Experiments tab, the intensity image data shown on the image display (LAS AF
Viewer) is saved. The FLIM data is only saved in the PicoQuant software SymPhoTime.
• On the Acquisition tab, you configure the settings for image acquisition such as scan
speed, zoom, or pinhole diameter or you can define a stack or time series for FLIM
measurements.
• On the Setup tab, you can select external or internal detectors for FLIM, run FLIM test
measurements, and edit file names and comments.
After completing the FLIM measurements, you can close the Wizard by selecting the TCS
SP8 operating mode (Figure 88).
Figure 88:
Closing the Wizard
LAS AF
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