Leica TCS SP8 SMD User Manual page 125

For fcs, flim and flcs
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3. You can activate up to eight laser lines simultaneously. Click the corresponding buttons
(Figure 108, item 2).
The position of the laser lines in the spectrum can be moved as desired.
4. Adjust the power of the line by adjusting the slider for the respective laser line.
5. Select Constant Power mode for excitation scans using the white light laser.
This sets the laser power automatically to 100 %.
When Constant Percentage (Figure 108, item 3) is selected, work can be done using less
power (e.g. 70 %).
Figure 108:
Adjusting Constant Percentage
6. Then, click on the nearby button (Figure 108, item 4) to call up the laser configuration
window.
7. In the Currently available Lasers dialog, you can set the laser power (Figure 109). To do
this, enable the laser (Figure 109, item 1). Set the desired laser power using the slider
(Figure 109, item 2).
o
Figure 109:
Settings in the Settings menu
Selecting the Laser
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