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power switch is in the "ON" position.
3. Start the laser driver and switch the laser on. If you are using the PDL 828 Sepia II laser
driver, set the key switch to the "ON" position. Prior to data acquisition, the laser head
should be operated for a few minutes to stabilize. If you use a PDL 828 Sepia II, start the
specific Sepia software on the SMD workstation and set the laser settings there (refer
to corresponding manuals).
4. Start the SymPhoTime software on the SMD workstation.
5. Open an existing workspace by selecting Load Workspace in the File menu or create a
new workspace by selecting New Workspace in the File menu (see SymPhoTime
manual).
6. Set the correct fluorescence filter on the corresponding filter holder position of the
external PicoQuant detector unit or on the Leica TCS SP8. Select the correct SMD filter
cube in the X1 port adapter (Chapter 23, Figure 188). If you have a dual-channel APD
detector unit, set the variable beam splitter unit to the correct position (see dual channel
detector unit manual for details).
7. Be sure that no light falls on the detectors. Either switch on the power supply or, if using
a DSN 102 power supply, start the respective detector by selecting the corresponding
channel button on the DSN 102.
The SMD upgrade is now ready to use. The communication between the SP8 confocal
system itself and the SMD upgrade electronics starts at the beginning of the data
acquisition.
Switching On the System
101
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