Leica HistoCore AUTOCUT Instructions For Use Manual
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Leica HistoCore AUTOCUT Instructions For Use Manual

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HistoCore AUTOCUT
Rotary Microtome
Instructions for Use
English
Order No.: 14 0519 80101 - Revision B
Always keep this manual with the instrument.
Read carefully before working with the instrument.

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Summary of Contents for Leica HistoCore AUTOCUT

  • Page 1 HistoCore AUTOCUT Rotary Microtome Instructions for Use English Order No.: 14 0519 80101 - Revision B Always keep this manual with the instrument. Read carefully before working with the instrument.
  • Page 3 These are determined only by the contract provisions agreed between ourselves and our customers. Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products.

  • Page 4: Table Of Contents

    Table of Contents Important Information ..........................Symbols used in the text and their meanings ..................... 7 Instrument type ..............................9 Intended use ..............................9 Qualification of personnel ..........................9 Safety ................................Safety notes ..............................10 Warnings ................................. 10 2.2.1 Markings on the instrument itself ....................... 11 2.2.2 Transport and installation ..........................
  • Page 5 6.3.3 Two-in-one blade holder E ........................... 78 6.3.4 Knife holder E with a water trough for low-profile blades ..............79 6.3.5 Knife holder N/NZ ............................80 Section waste tray ............................82 Backlighting ..............................82 Top tray ................................83 Universal microscope carrier ........................83 HistoCore AUTOCUT...
  • Page 6 Table of Contents Magnifying lens, LED illumination ....................... 86 Additional accessories ..........................88 6.10 Ordering information ........................... 100 Troubleshooting ............................Error codes ..............................102 Possible faults .............................. 103 Instrument malfunctions..........................105 Malfunctions of the two-in-one blade holder E ..................107 7.4.1 Replacing the pressure plate........................

  • Page 7: Important Information

    Symbol for labeling electrical and electronic equipment in accordance with Section 7 of the German Electrical and Electronic Equipment Act (ElektroG). ElektroG is the law on the bringing into circulation, return and environmentally compatible disposal of electrical and electronic equipment. HistoCore AUTOCUT...
  • Page 8 Important Information The CSA test mark means that a product has been tested and fulfills the applicable safety and/or performance standards, including the relevant standards defined or administered by the American National Standards Institute (ANSI), Underwriters Laboratories (UL), the Canadian Standards Association (CSA), the National Sanitation Foundation International (NSF) and others.

  • Page 9: Instrument Type

    Intended use The HistoCore AUTOCUT is a fully automatic, motorized rotary microtome with a separate control panel specifically designed for creating thin sections of human tissue specimens of varying hardness for histological medical diagnosis, e.g. cancer diagnosis. It is intended for sectioning soft and hard human specimens, as long as they are suitable for being cut motorized or manually.

  • Page 10: Safety

    Instructions for Use. The safety and caution notes in this chapter must be observed at all times. Be sure to read these notes even if you are already familiar with the operation and use of other Leica Biosystems products.

  • Page 11: Markings On The Instrument Itself

    Ground needs to be vibration-free as far as possible. • Always make sure that all 4 feet are positioned completely on the laboratory table. • If the instrument has been potentially moved by other persons (for example for servicing) always double-check correct positioning. HistoCore AUTOCUT...
  • Page 12 Safety Warning Condensation inside the instrument due to exposure to extreme differences in temperature and high air humidity. Damage of the instrument. • Always make sure that for both storage and operation appropriate climate conditions are met. See the section of Technical data (→...

  • Page 13: Operating The Instrument

    Serious injury of persons and/or property damage including specimen damage. • Never remove or modify any protective devices of the instrument or any accessories. Only service personnel qualified by Leica Biosystems may repair the instrument and access the instrument's internal components.
  • Page 14 Safety Warning Personal protective equipment is not used. Injury of persons. • When working with microtomes, personal safety precautions must always be taken. It is mandatory to wear work safety shoes, safety gloves, a mask and safety goggles. Warning Knives or blades are handled and/or disposed in an inappropriate manner. Serious injury of persons due to contact with extremely sharp knife/blade.
  • Page 15 Working with the instrument and brittle specimen without appropriate protective clothes. Serious injury of persons by splinters while cutting brittle specimen. • Always wear appropriate protective clothes (including safety goggles) and work especially carefully when cutting brittle specimen. HistoCore AUTOCUT...
  • Page 16 • Make sure that all prerequisites for appropriate sectioning are met. Refer to the troubleshooting section of these Instructions for Use for further details. • If you do not have sufficient knowledge in regards of troubleshooting insufficient sectioning results consult persons who do have that knowledge, for example, application experts of Leica Biosystems. Warning Wheel turn direction of electric coarse feed incorrectly selected.
  • Page 17 Emergency stop does not work after software crash. Specimen damage. • Try to restart the instrument. • If restart fails, unplug the power cord and contact Leica Biosystems service representative. Warning Improper fix to software freeze. Specimen damage and/or delay of diagnose.
  • Page 18 Safety Warning Misinterpretation of the yellow LED in the field. M-STOP Serious injury and/or property damage. • Operate the instrument only after you have completely understood the meaning of the 3 possible LED indications. Extinguished light: the instrument is ready to use (no brake engaged); blinking light: the electronic brake is engaged, but mechanical locking system is deactivated;...
  • Page 19 Using the electronic brake to lock the handwheel. People injury / Damage to specimen. • Electronic brake is a brake mechanism which cannot work as a security lock. The handwheel is securely locked only when the handwheel locking mechanism is in the 12 o'clock position. HistoCore AUTOCUT...

  • Page 20: Cleaning And Maintenance

    • Ensure that liquids do not enter the interior of the instrument during operation and maintenance. Spray proper solvents or cleaners on a cleaning pad and not directly on the instrument to prevent liquids from entering. If liquids go into interior of instrument, contact Leica Biosystems service. Warning Wiping the knife in the wrong direction during cleaning.

  • Page 21: Integrated Protective Devices

    Serious injury of persons and/or property damage including specimen damage. • Never remove or modify any protective devices of the instrument or any accessories. Only service personnel qualified by Leica Biosystems may repair the instrument and access the instrument's internal components.

  • Page 22: Handwheel Lock (Manual Operation Only)

    Safety 2.3.2 Handwheel lock (manual operation only) Fig. 2 Fig. 3 There are two ways of locking the handwheel (→ "Fig. 2-1"): • Using the handwheel locking mechanism (→ "Fig. 2-2") on the top of the handwheel, the handwheel can be braked in the 12 o'clock position. 1.

  • Page 23: Safety Guard On The Knife/Blade Holder

    The safety guard (→ "Fig. 6-1") of the knife holder NZ can be easily positioned via two handles (→ "Fig. 6-2"). To cover the knife edge, push both cover strips of the safety guard to the center. Fig. 6 HistoCore AUTOCUT...
  • Page 24 Safety Knife holder E-TC The safety guard on knife holder E-TC consists of a foldaway handle (→ "Fig. 7-1"). To cover the cutting edge, fold the safety guard handle upwards. Fig. 7 Two-in-one blade holder E for high and low profile blades The safety guard on the two-in-one blade holder E consists of a red foldaway handle...

  • Page 25: Instrument Components And Specifications

    Microtome base 12 Handwheel Blade holder base 13 Handwheel locking mechanism Two-in-one blade holder E 14 Instrument control panel Clamping lever of the knife/blade holder base 15 Top tray (optional) Section waste tray (available as standard or anti-static) HistoCore AUTOCUT...

  • Page 26: Instrument Specifications

    • Horizontal feed and vertical stroke mechanisms are based on cross roller bearings. • The HistoCore AUTOCUT is primarily designed for motorized sectioning, but is also suitable for manual sectioning applications. • Smooth-running hand-wheel allows two manual sectioning modes: rocking mode and conventional manual sectioning with full hand-wheel rotation, and 3 motorized sectioning modes: single, continuous and step.
  • Page 27 2. the fast homing function of the object head within 13 +/- 2 seconds from front to rear position. • Large top surface area allows placement of objects that require a flat surface. • Optional removable top tray allows storage of sectioning tools and prevents items from falling. HistoCore AUTOCUT...

  • Page 28: Technical Data

    Instrument Components and Specifications Technical Data General Nominal supply voltages 100/120/230/240 V AC ±10% Nominal frequency 50/60 Hz Maximum power consumption 100 VA Protection class (according to IEC-1010, UL 3101, EN 61010) Power fuses 2 x T 3.15 A Pollution degree (according to IEC-1010, UL 3101, EN 61010) Overvoltage category (according to IEC-1010, UL 3101, EN 61010)
  • Page 29 Optional accessories Specimen orientation with zero postion • Horizontal rotation: ± 8° • Vertical rotation: ± 8° Two-in-one blade holder -Lateral displacement function 3 positions East-west movement -Movement of blade holder base: North-south: ± 24 mm HistoCore AUTOCUT...

  • Page 30: Setting Up The Instrument

    14 0502 37717 Quick clamping system 14 0502 37718 Universal cassette clamp 14 0502 37999 Blade holder base 14 0502 55546 2-in-1 Blade holder E 14 0502 54497 HistoCore AUTOCUT basic instrument includes the following delivery list. Version 1.1, Revision B...
  • Page 31 To have a workable configuration, at least one item of the categories mentioned below must be ordered. Basic instrument 14051956472 Basic instrument HistoCore AUTOCUT without all of the HistoCore AUTOCUT following: orientation, quick clamping system, specimen clamp, blade or knife holder set-up and waste tray...

  • Page 32: Unpacking And Installation

    The accessories ordered are included in a separate box. Please check all delivered parts against the packing list and against your order to verify whether the delivery is complete. Should you find any discrepancies, please contact your Leica Biosystems sales office without delay.
  • Page 33 The transport carton and included retaining elements should be kept in case a return shipment is necessary later. To return the instrument, follow the instructions below in reverse order. • Remove the packing strap and the adhesive tape (→ "Fig. 11-1"). • Remove the carton lid (→ "Fig. 11-2"). Fig. 11 HistoCore AUTOCUT...
  • Page 34 Setting up the instrument • Take out the accessory carton (optional accessories) (→ "Fig. 12-3") and the cartons (→ "Fig. 12-4") for the standard scope of delivery. Fig. 12 • Take out the fixing module (→ "Fig. 13-5"). To do so, hold it by the top edge of the module and in the recessed grip (→...

  • Page 35: Assembling The Handwheel

    Connecting the instrument to a non-grounded socket, or use an extension cord. Risk of electrical shock which causes injury to persons, or delayed diagnosis. • The instrument must be connected to a grounded socket. • Do not use an extension cord. HistoCore AUTOCUT...

  • Page 36: Checking The Voltage

    Setting up the instrument 4.5.1 Checking the voltage The HistoCore AUTOCUT can be connected to various electrical power grids (depending on voltage and frequency). New instruments are factory-set to 230 volts. This is documented by a yellow label (230 VOLT) on the rear of the instrument, which covers the power switch and power socket.

  • Page 37: Connecting The Dummy Plug

    If neither the dummy plug nor the foot switch is connected, the instrument is not ready for operation. In this case, the emergency stop function is activated and the E-STOP (→ "Fig. 18-4") on the instrument is illuminated; the memory position is erased and the three-digit display indicates shortly. HistoCore AUTOCUT...

  • Page 38: Connecting The Control Panel

    Setting up the instrument 4.5.5 Connecting the control panel Fig. 19 The connecting cable (→ "Fig. 19-1") of the control panel is fixed to the microtome. It may not be disconnected. 1. Insert the plug (→ "Fig. 19-2") of the connecting cable into the connecting jack (→...
  • Page 39 If the yellow LED in the field of the control panel is flashing, M-STOP the electronic brake is activated. The instrument cannot be used as long as the LED is lit or flashing. HistoCore AUTOCUT...

  • Page 40: Operation

    • If instrument cannot set back into operation immediately, make sure that the specimen is stored in an appropriate manner to avoid any damage to it. • If required, contact Leica Biosystems service representative. 5.1.1 Instrument control panel Fig. 20 Lights up during specimen retraction.

  • Page 41: Separate Control Panel

    • In the trimming/sectioning mode: coarse feed backward fast; fast backward • In the sectioning mode (Step mode activated): multiple step backward. Yellow LED • Flashes during coarse feed forwards; • Lights up when the remaining feed area is reached. HistoCore AUTOCUT...
  • Page 42 Operation Coarse feed button - • In the trimming/sectioning mode: coarse feed forward fast; fast forward • In the sectioning mode (Step mode activated): multiple step forward. Coarse feed button - • In the trimming/sectioning mode: coarse feed backward slow; slow backward •...

  • Page 43: Display And Control Elements

    The instrument is delivered with continuous feed (standard configuration). Fast homing Press the fast-backward coarse feed button (→ "Fig. 22-1"), the object head is Fig. 22 moving from the front end position to the home at 1800µm/s. HistoCore AUTOCUT...
  • Page 44 Operation Caution Turning the electronic coarse feed wheel during fast homing or moving to the memory position. Specimen impaired. • Do not touch the electronic coarse feed wheel during fast homing or moving to the memory position. Caution Turning the handwheel or pressing buttons on the separate control panel or the instrument control panel during fast homing or moving to the memory position.
  • Page 45 While the object head is moving to the memory position, the movement can be stopped by pressing MEMO button or any other buttons, or by rotating the electronic coarse feed wheel. button is enabled between the front limit position (not included) and home position (not MEMO included). HistoCore AUTOCUT...
  • Page 46 Operation Caution Manual movement of knife/blade holder and/or object head after setting-up the memory position. Damage of specimen. • Do not move the knife/blade holder, the holder base and/or object head and do not readjust the orientation without readjusting the memory position. Caution Turning the electronic coarse feed wheel during fast homing or moving to the memory position.
  • Page 47 (→ "Fig. 27-6"). A MENUMODE retraction movement takes place by the newly selected value after each section. • While the specimen is in retraction, the yellow LED (→ "Fig. 27-7") on the RETRACT display lights up. Fig. 27 HistoCore AUTOCUT...
  • Page 48 Operation • To switch off retraction, press the button (→ "Fig. 28-1") on the separate control panel until the display indicates (→ "Fig. 28-2"). • To exit the retraction settings, press MENUMODE (→ "Fig. 28-3"). When retraction is switched off, the specimen is not retracted. The yellow LED (→...
  • Page 49 If both the green and the yellow LEDs (→ "Fig. 35-3") in the button RUN/STOP are lit up, the cutting motor is still running; however it will stop in the next upper end position of the vertical stroke. Fig. 35 HistoCore AUTOCUT...
  • Page 50 Operation Electronic brake function The electronic brake function holds the object head in the upper position automatically at the end of the motorized sectioning, which prevents the handwheel from turning by accident after the sectioning is finished. When the electronic brake function is activated, the LED of the button lights BRAKE Fig.
  • Page 51 If the specimen head is already in the remaining feed range when the instrument is switched on, an additional acoustic signal is heard after the software version is displayed. HistoCore AUTOCUT...
  • Page 52 Operation • You can continue to work on the specimen by moving it back a short distance using the coarse feed buttons (set trimming mode!). • The STEP function is disabled in the remaining feed range. Foot switch (optional accessory) In addition to the foot switch, all control panel functions and all buttons on the instrument continue to be active.

  • Page 53: Electronic Coarse Feed Wheel

    Emergency stop does not work after software crash. Specimen damage. • Try to restart the instrument. • If restart fails, unplug the power cord and contact your Leica service representative. 5.1.4 Electronic coarse feed wheel The coarse motion serves for a fast horizontal forwards movement of the specimen - towards the knife - and backwards - away from the knife.
  • Page 54 Operation Fig. 50 The specimen orientation allows for simple position correction of the specimen surface when the specimen is clamped into place. The directional specimen holder fixture may be exchanged for a non-directional fixture (optional accessory). Display of the zero position For better display of the zero position, the orientation has two red indicators (→...

  • Page 55: Fine Adjustment Of The Force Balance

    • If fine adjustment is not properly set, do not work with the instrument but perform a readjustment. • Especially after changing accessories at the object head, perform a fine adjustment of the force balance immediately. HistoCore AUTOCUT...

  • Page 56: Inserting The Two-In-One Blade Holder E

    Operation The force balance is adjusted using the screw (→ "Fig. 52-1"), which can be accessed by removing the section waste tray on the bottom of the base plate of the microtome. Use the Allen key No. 5 (with handle) for the adjustment. •...

  • Page 57: Inserting The Two-In-One Blade Holder E

    1. Loosen the screw (→ "Fig. 55-3") using an Allen key No. 4 (→ "Fig. 55-6") until the two-in-one blade holder E (→ "Fig. 55-2") can be moved. HistoCore AUTOCUT...

  • Page 58: Inserting The Universal Cassette Clamp

    Operation 2. Move the two-in-one blade holder E until the index mark of the desired clearance angle coincides with the reference line on the blade holder base. Enlarged detail (→ "Fig. 55") shows a clearance angle setting of 5°. Tips The recommended clearance angle setting for the two-in-one blade holder E is approx.

  • Page 59: Clamping The Specimen

    Serious injury of persons due to contact with extremely sharp knives and/or blades. • Do not clamp two knives/blades on the knife holder/blade holder. Install the knife/blade in the center of the knife/blade holder. Knives/Blades must not exceed the edge of the knife/blade holder. HistoCore AUTOCUT...
  • Page 60 Operation Inserting high-profile blades Fig. 57 Fig. 58 1. Bring the safety guard (→ "Fig. 57-1") down. 2. To insert the blade, rotate the right clamping lever (→ "Fig. 57-2") counterclockwise. 3. Carefully push in the blade from above or from the side. Make sure that the blade is positioned in the center and, most importantly, parallel to the upper edge of the pressure plate(→...
  • Page 61 • Never place a knife anywhere with the cutting edge facing upwards and never try to catch a falling knife. • Always cover the edge of the knife/blade with the safety guard before clamping the specimen. HistoCore AUTOCUT...

  • Page 62: Trimming The Specimen

    Operation Detailed descriptions for inserting the knife into the individual knife holders are provided in (→ P. 68 – 6. Optional Accessories). Trimming the specimen 5.7.1 Trimming in manual operation Warning Wheel turn direction of electric coarse feed incorrectly selected. Specimen damage.

  • Page 63: Trimming In Motorized Operation

    (continuous stoke). • Release the handwheel locking mechanisim and the handwheel brake lever. • Start motorized sectioning and trim the specimen. • Terminate trimming when the desired sectioning surface and depth have been reached. Fig. 65 HistoCore AUTOCUT...

  • Page 64: Sectioning

    • Make sure that all prerequisites for appropriate sectioning are met. Refer to the troubleshooting section of these Instructions for Use for further details. • If you do not have sufficient knowledge in regards of troubleshooting insufficient sectioning results consult persons who do have that knowledge, for example, application experts of Leica Biosystems. Warning Turning the handwheel counterclockwise.
  • Page 65 • Depending on the operation planned, use the button to select CUT MODE one of the motorized sectioning modes, CONT, SINGLE or STEP. • Check the sectioning speed setting and set an appropriate speed. • Start motorized sectioning. • Pick up the sections. Fig. 66 HistoCore AUTOCUT...

  • Page 66: Changing The Specimen Or Interrupting Sectioning

    Operation Changing the specimen or interrupting sectioning Warning The handwheel is unlocked and the object head drops into knife/blade holder. People injury/Damage to specimen. • Except sectioning phase, the handwheel must be locked at all times. Warning Specimen is oriented in retraction phase. Damage of specimen and/or knife/blade.
  • Page 67 4. Move the object head to the rear end position or move the knife holder on the knife holder base outwards. 5. Push all section debris into the section waste tray and empty the tray. 6. Switch the instrument off at the main power switch. 7. Clean the instrument (→ P. 109 – 8.1 Cleaning the instrument). HistoCore AUTOCUT...

  • Page 68: Optional Accessories

    Optional Accessories Optional Accessories Assembly for fixture for specimen clamps Tips Depending upon the purchase order, the basic instrument is delivered with the fine directional, directional or rigid fixture for specimen clamps which must be assembled first. All specimen clamps available as accessories can be used in one of the three fixtures for specimen clamps.

  • Page 69: Directional Fixture For Specimen Clamps

    Allen key No. 3, fasten the baseplate to the object head (→ "Fig. 70-4"). • Now, screw the fine-directional fixture for specimen clamps with the 4 screws (→ "Fig. 70-2")and the Allen key No. 3 onto the object head. Fig. 70 HistoCore AUTOCUT...

  • Page 70: Quick Clamping System

    Optional Accessories Tips If the fine-directional fixture for specimen clamps is not used, retain the baseplate (→ "Fig. 70-1") 4 screws (→ "Fig. 70-3") together with the fine-directional fixture for specimen clamps. 6.1.4 Quick clamping system It is used as an adaptor for use with the fine- directional fixture for specimen clamps with zero point indicators or the directional fixture for specimen clamps.

  • Page 71: Standard Specimen Clamp

    Instructions for Use for further details. • If you do not have sufficient knowledge in regards of troubleshooting insufficient sectioning results consult persons who do have that knowledge, for example, application experts of Leica Biosystems. HistoCore AUTOCUT...

  • Page 72: V-Insert

    Optional Accessories 6.2.2 V-insert The V-insert (→ "Fig. 73-4") is mounted in the hole provided in the lower movable jaw of the standard specimen clamp. This makes it possible to clamp round specimens in the standard specimen clamp. • Turn the knurled screw (→...

  • Page 73: Universal Cassette Clamp

    Instructions for Use for further details. • If you do not have sufficient knowledge in regards of troubleshooting insufficient sectioning results consult persons who do have that knowledge, for example, application experts of Leica Biosystems. HistoCore AUTOCUT...
  • Page 74 Optional Accessories Surgipath cassettes with minimum dimensions of 39.8 x 28 mm and maximum dimensions of 40.9 x 28 mm may be clamped in the universal cassette clamp (UCC) horizontally as well as vertically. • Pull the lever (→ "Fig. 76-1") towards the operator.

  • Page 75: Super Mega-Cassette Clamp

    Inserts for specimens of 6, 15 and 25 mm diameter are available. The holder for round specimens can be mounted on the rigid fixture for specimen clamps or the fine directional or directional fixtures for specimen clamps with quick clamping system. HistoCore AUTOCUT...

  • Page 76: Knife Holder Base And Knife Holder

    Optional Accessories • To mount the required insert (→ "Fig. 78-1") (→ "Fig. 78-2") (→ "Fig. 78-3"), turn the clamping ring (→ "Fig. 78-4") counterclockwise and remove it. • Place the required insert into the clamping ring (→ "Fig. 78-4") and screw the clamping ring onto the thread (→...

  • Page 77: Knife Holder Base, Without Lateral Displacement Feature

    • Carefully insert the blade from the side. Make sure that the brilliant shining facet is facing you and the blade is clamped parallel to the upper edge of the pressure plate. • To clamp the blade, rotate clamping lever Fig. 81 (→ "Fig. 81-2") clockwise. HistoCore AUTOCUT...

  • Page 78: Two-In-One Blade Holder E

    Optional Accessories 6.3.3 Two-in-one blade holder E Fig. 82 Blade ejector Safety guard Clamping top Bolt Clamping lever (left) Clamping lever (right) Segment arc Pressure plate Blade holder base The two-in-one blade holder E is designed for conventional disposable blades and optimized to be used with Surgipath disposable blades.

  • Page 79: Knife Holder E With A Water Trough For Low-Profile Blades

    84-3")) must remain in the position shown at all times, as otherwise isolated malfunctions of the knife holder can occur. Clamping lever for the blade (→ "Fig. 84-2") is at the right, clamping lever for the lateral displacement (→ "Fig. 84-3") is at the left. HistoCore AUTOCUT...

  • Page 80: Knife Holder N/Nz

    Optional Accessories Floating thin paraffin sections (for example, for subsequent immunostaining procedures) on the surface of the water. The vessel is filled with distilled or deionized water up to the blade. After trimming, remove the section waste from the vessel and create the sections to be prepared.
  • Page 81 • After the knife has been repositioned, adjust the knife height (→ P. 81 – Knife height adjustment), then tighten the clamping screw (→ "Fig. 86-4") which is located on the side to by turning it clockwise to clamp the knife (→ "Fig. 86-5"). HistoCore AUTOCUT...

  • Page 82: Section Waste Tray

    Optional Accessories Caution After the knife/blade holder is laterally displaced, the specimen is not retracted and retrimmed. Specimen damage. • Retract the object head and retrim the specimen block each time after laterally displacing the knife/blade holder. Section waste tray Standard section waste tray and anti-static section waste tray are available.

  • Page 83: Top Tray

    The top tray is for storage of the utensils used during sectioning as well as the sectioned specimens. Fig. 91 Universal microscope carrier Tips Unpack all accessories in the package and check for completeness. HistoCore AUTOCUT...
  • Page 84 Optional Accessories • (→ "Fig. 92-1"), Baseplate with drilling (→ "Fig. 92-11") • (→ "Fig. 93-2"), Vertical column with size 8 cap screw (→ "Fig. 93-12") and lock washer (→ "Fig. 93-13") • (→ "Fig. 93-3"), Horizontal arm with cross- member (→...
  • Page 85 • After installing the vertical column, immediately place the microtome onto the baseplate so that the front feet of the microtome are positioned in the shallow recesses (→ "Fig. 96-19"). Tips The lock washer must be positioned between the baseplate and vertical column to prevent unintentional turning of the column. HistoCore AUTOCUT...

  • Page 86: Magnifying Lens, Led Illumination

    Optional Accessories • Attach horizontal arm. Slide the support ring (→ "Fig. 97-15") onto the vertical column and position it so that the lock nut (→ "Fig. 97-16") facing to the back. Tighten the lock nut. Slide the cross-member (→ "Fig. 97-14") onto the column.
  • Page 87 • Always cover the magnifier lens when not working with it. • Especially protect it from exposure to direct sunlight. • Use the provided protective cap (→ "Fig. 98-4") to cover the magnifying lens. HistoCore AUTOCUT...

  • Page 88: Additional Accessories

    Optional Accessories Additional accessories Knife holder base, non-orientable Silver for knife holders N, NZ, E-TC and knife holder E with trough • Order No.: 14 0502 37962 Fig. 100 Knife holder N Silver, for holding conventional knives up to 16 cm in length Height adjustment of the knife Separate clearance angle adjustment...
  • Page 89 Rustproof clamping plate made of stainless steel • Order No.: 14 0502 37997 Fig. 104 Blade holder base for two-in-one blade holder E Blade holder base for two-in-one blade holder E • Order No.: 14 0502 55546 Fig. 105 HistoCore AUTOCUT...
  • Page 90 Optional Accessories Two-in-one blade holder E For high and low profile disposable blades For HistoCore AUTOCUT Ability to shift the clamping top laterally 3 click-stop positions make it easier to utilize the entire blade length The pressure plate is replaceable.
  • Page 91 • Order No.: 14 0216 07132 Fig. 110 Knife, 16 cm, profile d, tungsten carbide Knife, 16 cm long, tungsten carbide, profile d Note: Knife case 14 0213 11140 included • Order No.: 14 0216 04813 Fig. 111 HistoCore AUTOCUT...
  • Page 92 Optional Accessories Knife 16 cm, profile c, tungsten carbide Knife, 16 cm, tungsten carbide, profile c Note: Knife case 14 0213 11140 included • Order No.: 14 0216 04206 (→ "Fig. 112-1") is the carbide metal knife serial number. Fig. 112 Knife case Variable knife case (plastic), for 1 or 2 knives: 10 - 16 cm long...
  • Page 93 • Order No.: 14 0502 38949 Fig. 117 Specimen holder fixture, non-directional Silver, for holding a variety of specimen clamps (→ P. 68 – 6. Optional Accessories), including installation hardware • Order No.:14 0502 38160 Fig. 118 HistoCore AUTOCUT...
  • Page 94 Optional Accessories Specimen holder fixture, fine directional Silver, with 2 zero point indicators, XY orientation 8° per direction, click stops every 2° Note: Quick clamping system 14 0502 37718 must be ordered separately. • Order No.: 14 0502 37717 Fig. 119 Quick clamping system For specimen holder for use with the fine- directional fixture for specimen clamps with zero...
  • Page 95 EM universal specimen holder For samples with a 8.5 mm diameter • Order No.: 14 0356 10868 Special wrench (ordered separately) with Order No.: 14 0356 10869 can be used to clamp specimen Fig. 125 in EM holder HistoCore AUTOCUT...
  • Page 96 Optional Accessories EM flat specimen holder Opening width up to 4.5 mm • Order No.: 14 0355 10405 Special wrench (ordered separately) with Order No.: 14 0356 10869 can be used to clamp specimen Fig. 126 in EM holder Super mega cassette clamp With adapter, silver Note: Use only together with non-orientable specimen...
  • Page 97 Power supply with cable and 4 adapters (UK, US, EU, AUS) 5 cable clamps Instructions for Use and DVD • Order No.: 14 0502 46573 Tips The RM Coolclamp cannot be used in connection with a microscope or magnifier. HistoCore AUTOCUT...
  • Page 98 Optional Accessories Magnifier For assembly on microscope carriers (14 0502 40580), 2x magnification Note: Magnifier includes an adapter for holding the optional LED illumination • Order No.: 14 0502 42790 Fig. 129 Universal microscope carrier Universal assembly • Order No.: 14 0502 40580 Fig.
  • Page 99 • Anti-static section waste tray Order No.:14 0517 56237 • Section waste tray Order No.:14 0518 56458 Fig. 133 Brush With magnet for blade removal tool for two-in-one blade holder E. • Order No.: 14 0183 40426 Fig. 134 HistoCore AUTOCUT...

  • Page 100: Ordering Information

    Optional Accessories Safety gloves Size M, 250 ± 20mm, Yellow • Order No.: 14 0340 29011 Cut resistant safety gloves, size S, 250±20mm • Order No.: 14 0340 40859 Fig. 135 6.10 Ordering information Knife holder base 14 0502 37962 Knife holder N 14 0502 37993 Knife holder NZ...
  • Page 101 Foot switch for rotary microtomes 14 0502 38257 Brush with magnet 14 0183 40426 Safety gloves, size M 14 0340 29011 Safety gloves, size S 14 0340 40859 For more consumables, please visit the product online catalog on our website www.LeicaBiosystems.com. HistoCore AUTOCUT...

  • Page 102: Troubleshooting

    2.Check all setting values to see if the previously entered values are still correct. The values may have to be reentered. 3.If the error code appears repeatedly, call Leica Biosystems service. Error code Object head feeding 1.Restart the instrument and check displayed; a two-...

  • Page 103: Possible Faults

    In this case, have Technical Service inspect and reconfigure the universal cassette clamp. HistoCore AUTOCUT...
  • Page 104 Leica Biosystems, or the specimen is torn out of the cassette may become the embedding. In extreme deformed or other clamping cases, there are no sections problems may arise.

  • Page 105: Instrument Malfunctions

    The following is a list of the most common problems which can arise while working with the instrument, along with possible causes and troubleshooting procedures. If the malfunction cannot be remedied with any of the options listed in the table, or the problem occurs repeatedly, inform Leica Biosystems Service Support immediately.
  • Page 106 • Release both buttons RUN/STOP ENABLE buttons of the control simultaneously. panel were not released simultaneously after starting. HOME and STOP are lit up • The end position detection is • Contact Leica Biosystems simultaneously. defective. service. Version 1.1, Revision B...

  • Page 107: Malfunctions Of The Two-In-One Blade Holder E

    (→ "Fig. 138-5") in case it falls out. If it is installed wrongly, the pressure plate cannot be clamped. 5. Install a new pressure plate. 6. Insert the bolt (→ "Fig. 138-3"). 7. Rotate clamping lever clockwise (→ "Fig. 138-2"). HistoCore AUTOCUT...

  • Page 108: Mounting The Clamping Top Onto The Segment Arc

    Troubleshooting 7.4.2 Mounting the clamping top onto the segment arc Fig. 139 To ensure a proper sectioning result, the clamping top (→ "Fig. 139-2") must be securely clamped onto the segment arc (→ "Fig. 139-4"). The clamping is carried out using an eccentric lever (→...

  • Page 109: Cleaning And Maintenance

    • Ensure that liquids do not enter the interior of the instrument during operation and maintenance. Spray proper solvents or cleaners on a cleaning pad and not directly on the instrument to prevent liquids from entering. If liquids go into interior of instrument, contact Leica Biosystems service. Warning Knife/blade was not removed when knife/blade holder was uninstalled from the microtome.
  • Page 110 Cleaning and Maintenance Caution Use of improper solvents or cleaners, or sharp/hard tools to clean the instrument or accessories. Potential instrument malfunction or delay of diagnosis. • Do not use any solvents containing acetone or xylene for cleaning. • When using cleaners, please comply with the safety instructions of the manufacturer and the laboratory safety regulations.
  • Page 111 4. Loosen the eccentric bolt using Allen key No. 4 and remove the segment arc from the blade holder base. 5. Clean all parts of the two-in-one blade holder E. Caution During cleaning, the parts of blade holders are mixed up. Poor section quality. • Do not mix up blade holders during cleaning. HistoCore AUTOCUT...

  • Page 112: Maintenance

    Cleaning and Maintenance 6. Lay the removed parts on an absorbent cloth into the drying chamber (up to a max. of 65 °C) and let the paraffin contamination run off. Warning Removing the parts from the drying chamber (65 °C) during cleaning of the knife/blade holder. Scalding hazard.

  • Page 113: Maintenance Instructions

    3. Have the instrument inspected on a regular basis by a qualified service technician authorized by Leica Biosystems. The intervals depend on how heavily the instrument is used. We recommend the following inspection intervals depending on the general workload of the instrument...

  • Page 114: Lubricating The Instrument

    3 seconds in the three-digit display when the TRIM/SECT button is pressed (switching between trimming and sectioning mode). This is a reminder that the instrument must be inspected by an authorized Leica Biosystems service technician, regardless of how heavily the instrument has been used.
  • Page 115 • Clamping lever (→ "Fig. 146-2") for the blade. Universal cassette clamp Fig. 147 Lubricate the spiral springs (→ "Fig. 147-1") of the tension lever (→ "Fig. 147-2") and move it back and forth several times. HistoCore AUTOCUT...

  • Page 116: Warranty And Service

    Service information If you are in need of technical customer support or spare parts, please contact your Leica Biosystems representative or the Leica Biosystems dealer where you purchased the instrument. Please provide the following information: •...

  • Page 117: Decontamination Confirmation

    Decontamination Confirmation Decontamination Confirmation Every product that is returned to Leica Biosystems or that requires on-site maintenance must be properly cleaned and decontaminated. You can find the dedicated template of the decontamination confirmation on our website www.LeicaBiosystems.com within the product menu. This template has to be used for gathering all required data.
  • Page 118 Notes Version 1.1, Revision B...
  • Page 120 Leica Biosystems Nussloch GmbH Heidelberger Str. 17 - 19 D-69226 Nussloch Germany Tel.: +49 - (0) 6224 - 143 0 Fax: +49 - (0) 6224 - 143 268 Web: www.LeicaBiosystems.com...

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