Leica CM1520 Instructions For Use Manual
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Leica CM1520 Instructions For Use Manual

Cryostat
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Leica CM1520
Cryostat
Instructions for Use
English
Order No.: 14 0491 81101 - Revision K
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Version 2.0, Revision K - 10.2019

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  • Page 1 Leica CM1520 Cryostat Instructions for Use English Order No.: 14 0491 81101 - Revision K Always keep this manual with the instrument. Read carefully before working with the instrument. Version 2.0, Revision K - 10.2019...
  • Page 3 Only in this way is it possible to continuously improve our technology and manufacturing techniques used in our products. This document is protected under copyright laws. All copyrights to this documentation are held by Leica Biosystems Nussloch GmbH.

  • Page 4: Table Of Contents

    Maintenance ..........................16 Technical Data ......................Installing the Instrument ..................... Installation site requirements ....................20 Unpacking instructions for the Leica CM1520 cryostat .............. 21 Standard delivery - packing list ....................24 Installing the handwheel ......................25 Instrument Setup ....................... Electrical connection ........................ 26 Preparations for starting up the instrument ................
  • Page 5 10.2 Mobile heat extractor - application .................... 94 10.3 Cold extraction - using the Thermal block .................. 94 10.4 Stationary heat extractor (optional) - application ............... 95 10.5 Installing the shelf (movable) (optional) ..................96 Warranty and Service ....................12. Decontamination Certificate ..................Leica CM1520...

  • Page 6: Important Information

    Symbols used in the text and their meaning Warning Leica Biosystems GmbH assumes no liability for consequential loss or damage due to failure to observe the following instructions, particularly in relation to transportation and package handling, and failure to observe the instructions for handling the instrument carefully.
  • Page 7 Title of the symbol: Serial number Description: Indicates the manufacturer's serial number so that a specific medical device can be identified. Symbol: Title of the symbol: Consult Instructions for Use Description: Indicates the need for the user to consult the Instructions for Use. Leica CM1520...
  • Page 8 Important Information Symbol: Title of the symbol: Flammable liquid Description: Package labeling in accordance with German Hazardous Freight Ordinance Road and Rail (GGVSE)/European Agreement concerning the International Carriage of Dangerous Goods by Road (ADR) for transporting hazardous goods. Class 3: FLAMMABLE LIQUID. Symbol: Title of the symbol: Flammable freezing sprays prohibited...

  • Page 9: Qualification Of Personnel

    Qualification of personnel The Leica CM1520 may only be operated by trained laboratory personnel. Prior to operating the instrument, the operator must carefully read and understand these Instructions for Use and must familiarize him/herself with all technical details of the instrument. The instrument is intended for professional use only.

  • Page 10: Instrument Type

    Important Information Instrument type All information contained in these Instructions for Use applies exclusively to the instrument type indicated on the title page. A nameplate bearing the instrument serial number (SN) is attached to the back of the instrument. These Instructions for Use include important instructions and information related to the operating safety and maintenance of the instrument.

  • Page 11: Safety

    The safety and caution notes in this chapter must be observed at all times. Be sure to read these notes even if you are already familiar with the operation and use of other Leica products. These Instructions for Use include important instructions and information related to the operating safety and maintenance of the instrument.

  • Page 12: Hazard Warnings

    Safety Hazard warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention. Operating the instrument safely is, above all, the responsibility of the owner, as well as the designated personnel who operate, service or repair the instrument. To ensure trouble-free operation of the instrument, make sure to comply with the following instructions and warnings.

  • Page 13: Safety Guard

    • The instrument may not be operated in hazardous locations! • To ensure proper functioning of the instrument, it must be set up while maintaining a minimum distance on all sides from walls and furniture: - rear: 15 cm - right: 30 cm - left: 15 cm. Leica CM1520...

  • Page 14: Operating The Instrument

    Safety Electrical connection • Before connecting the instrument to an AC power outlet, ensure that the AC power of your laboratory matches the values on the nameplate of the instrument! • The minimum rated voltage must be maintained during the startup phase of the cooling system - (→...

  • Page 15: Cleaning And Disinfection

    Safety Cleaning and disinfection We strongly recommend defrosting the Leica CM1520 cryostat completely for approx. 24 hours, 1-2x annually or as needed. • It is not necessary to remove the microtome for routine disinfection. Note Remove section waste after EVERY change of specimen! Do not start disinfection until you have done this! Each new specimen is a potential source of contamination.

  • Page 16: Maintenance

    Safety Maintenance Replacing the fuses • Turn the instrument off and disconnect the power plug before replacing the fuses. • Use only the fuse types specified in (→ P. 17 – 3. Technical Data). Failure to comply with this can cause severe damage to the instrument and its surroundings! Version 2.0, Revision K...

  • Page 17: Technical Data

    600 x 722 x 1206 mm handwheel (width x depth x height) Overall size of device, with 730 x 722 x 1206 mm handwheel (width x depth x height) Working height (armrest) 1025 mm Overall size of packaging (width 960 x 820 x 1400 mm x depth x height) Empty weight (without 135 kg accessories) Leica CM1520...
  • Page 18 Technical Data Environmental specification Operating altitude Max. 2000 m above sea level Operating temperature +18 °C to +35 °C Relative humidity (operation) 20 to 60 % RH non-condensing Transport temperature -29 °C to +55 °C Storage temperature +5 °C to +50 °C Relative humidity 10 to 85 % RH non-condensing (transport / storage) Minimum distance to walls Back: 150 mm Right: 300 mm Left: 150 mm Emmisions and boundary conditions Overvoltage category according to IEC 61010-1 Pollution degree...
  • Page 19 Hot gas defrosting Defrost duration 12 minutes Automatic turn-off defrost At chamber temperature of more than -5 °C At evaporator temperature of more than +38 °C Manual start Automatic start Manual abort Warning *) Refrigerant and compressor oil must be replaced by service personnel qualified and authorized through Leica only. Microtome Type Rotary microtome, encapsulated Section thickness range 2 µm to 60 μm Horizontal specimen feed 25 mm +3 mm Vertical specimen stroke 59 mm ±1 mm Specimen retraction...

  • Page 20: Installing The Instrument

    Installing the Instrument Installing the Instrument Installation site requirements Warning Do not operate the instrument in rooms with an explosion hazard! To ensure proper functioning of the instrument, it must be set up while maintaining a minimum distance on all sides from walls and furniture (→ P. 13 – Transport and installation).

  • Page 21: Unpacking Instructions For The Leica Cm1520 Cryostat

    Installing the Instrument Unpacking instructions for the Leica CM1520 cryostat Note • When the instrument is delivered, check the tilt indicators (→ "Fig. 3") on the packaging. • If the arrowhead is blue, the shipment was transported laying flat, was tilted at too great an angle or fell over during transport. Note this on the shipping documents and check the shipment for possible damage.
  • Page 22 Installing the Instrument Fig. 4 Fig. 5 Fig. 6 Fig. 7 Warning Do not slide the instrument by its hood (→ "Fig. 7-4") and observe the transport grip points (→ "Fig. 7-3"). The castors (→ "Fig. 7-2") must run over the ramp (→ "Fig. 7-1") at the front and rear. Risk of tipping! The instrument must be transported in an upright position. Version 2.0, Revision K...
  • Page 23 • To ensure a secure upright position at its intended location, the instrument must be adjusted. To do so, unscrew both adjusting feet (→ "Fig. 8-1") using the provided open-end wrench (size 13) (→ "Fig. 8-4"). • When subsequently transporting the instrument any further on castors, screw them back in all the way. Fig. 8 Leica CM1520...

  • Page 24: Standard Delivery - Packing List

    (English, with language CD 14 0491 81200) Compare the delivered components with the parts list and your order. Should you find any discrepancies, please contact your Leica sales office without delay. If the supplied local power cord is defective or lost, please contact your local Leica representative. Note A choice of different knife holders is available for the Leica CM1520. Version 2.0, Revision K...

  • Page 25: Installing The Handwheel

    (→ "Fig. 9-4") using the Allen key (6 mm). 4. Attach the cover disk (self-adhesive - not pictured). » To deinstall, proceed in reverse order. Warning Rotate the handwheel only if the cryostat is cooled and the cryochamber is cold. Leica CM1520...

  • Page 26: Instrument Setup

    Warning Use only the power cord provided by Leica. Failure to observe this causes an imminently hazardous situation that may result in death or serious injury to the operator or other personnel. • Check that the rubber plug is tight (in the drain hole below the left storage shelf), and press it tightly into the drain if necessary.
  • Page 27 Instrument Setup Warning We do not recommend placing disposable blade dispensers in the cryochamber for pre-cooling because it is possible that individual blades may stuck together during rempoval. They pose a risk of injury! Leica CM1520...

  • Page 28: General Overview Of The Leica Cm1520

    Instrument Setup General overview of the Leica CM1520 Fig. 10 Version 2.0, Revision K...
  • Page 29 Instrument Setup Fig. 11 Leica CM1520 12 Specimen disc Drain hose 13 Thermal block (optional) Control panel 1 14 Section waste tray Control panel 2 15 Brush shelf Storage shelf, left 16 Orientable specimen head Storage shelf, right 17 Blade/knife holder base (optional)

  • Page 30: Power Switch And Circuit Breaker

    Instrument Setup Power switch and circuit breaker • The switch must be in the top position (switch position (→ "Fig. 12") for switching on the circuit breaker (→ "Fig. 12-1"). • The switch must be in the bottom position (switch position 0) (→ "Fig. 12-2") for switching off the circuit breaker. Fig. 12 Switching on the instrument Warning...

  • Page 31: Operating The Instrument

    Use the function button labeled with a clock symbol to set the time of day. • Set the current time using the Plus (→ "Fig. 15-1") Minus (→ "Fig. 15-2") buttons. • Pressing and holding the Plus Minus button continuously increases or decreases the time (auto-repeat function). Leica CM1520...

  • Page 32: Setting The Automatic Defrost Time (Cryochamber)

    Operating the Instrument 6.2.2 Setting the automatic defrost time (cryochamber) Fig. 16 The automatic defrost cycle takes place once every 24 hours. • Briefly touch the Plus (→ "Fig. 16-1") Minus (→ "Fig. 16-2") button to display the beginning of the defrost time that is currently set. The two LEDs between the display of hours and minutes flash at the same time. • To change the beginning of the defrost time in 15-minute increments, touch or press and hold the Plus Minus button.

  • Page 33: Manual Defrosting Of The Quick Freeze Shelf

    The quick freeze shelf can become hot during the defrosting process. The defrost duration is 12 minutes. Note The quick freeze shelf and cryochamber cannot be defrosted at the same time. 6.2.5 Manual defrosting of the cryochamber Fig. 19 Leica CM1520...

  • Page 34: Setting The Section Thickness

    Operating the Instrument • You can activate manual defrosting of the cryochamber by pressing the key (→ "Fig. 19-1") (continuous tone sounds), followed immediately by the key (→ "Fig. 19-2") in the cryochamber temperature selection field (continuous tone stops). The display flashes during the defrosting (duration 12 min.). • To shut off the manual defrost early, press the (→ "Fig. 19-1") key, followed immediately by the (→ "Fig. 19-3") key in the cryochamber temperature selection field.

  • Page 35: Control Panel 2 - Electric Coarse Feed

    • Fast or slow feed toward the knife starts. The LED (→ "Fig. 22-2") starts flashing as long as the specimen head is in motion. The LED lights up when the forward end position has been reached (→ "Fig. 22-2"). Slow To feed the specimen, press and hold the appropriate Fig. 22 Slow Rapid button. Fast Leica CM1520...

  • Page 36: Daily Use Of The Instrument

    Daily Use of the Instrument Daily Use of the Instrument The sectioning process Preparation 1. Install all necessary parts, whether standard or optional (e.g. panels, waste and brush tray and the selected knife or blade holder). Make sure that they are mounted and cooled. 2.

  • Page 37: Quick-Freeze Shelf

    5. Once the specimen is frozen, insert the specimen disc (→ "Fig. 23-3") with the specimen into the specimen head (→ "Fig. 23-2") and start sectioning. Fig. 23 Note If the optional available stationary heat extractor is built in, see (→ P. 95 – 10.4 Stationary heat extractor (optional) - application). Leica CM1520...

  • Page 38: Specimen Discs

    Daily Use of the Instrument Specimen discs 7.3.1 Inserting the specimen discs into the specimen head 1. Lock the handle of the handwheel in the upper position. 2. If the knife/blade holder and a knife/blade are in place, cover the knife edge with the safety guard.

  • Page 39: Sectioning

    2. Adjust by screwing clockwise or counterclockwise the countersunk screw (→ "Fig. 26-3") in the clamping piece in the base using the Allen key No. 4, so that the eccentric lever (→ "Fig. 26-2") can be clamped at 0 ° position and 200 ° position.  Repeat this procedure until the knife holder base is securely clamped and not moving. Leica CM1520...

  • Page 40: Clearance Angle Adjustment

    Daily Use of the Instrument Fig. 26 7.4.2 Clearance angle adjustment Note • The harder the specimen is, the larger the clearance angle must be chosen. • However: The larger the clearance angle is, the larger the section buckling becomes. • If the clearance angle is too small, sections that are too thick or too thin can result. Also consider that the position of the knife edge relative to the specimen can change when the clearance angle is adjusted.

  • Page 41: Premium Blade Holder

    Daily Use of the Instrument Fig. 27 Note Settings of 2 ° - 5 ° (blade holder CE, CE-TC and premium blade holder) and 4 ° - 6 ° for the knife holder CN are well-suited for most applications. 7.4.3 Premium blade holder Fig. 28 Note Please observe the Instructions for Use included in the packaging for handling the premium blade holder. Leica CM1520...

  • Page 42: Blade Holder Ce

    Daily Use of the Instrument 7.4.4 Blade holder CE Note If additional blade holder Instructions for Use have been provided, read and follow the instructions and safety information carefully! Inserting the blade holder CE » Push the upper part of the blade holder onto the base. Clamp the blade holder into place on the left side using an Allen key (No.
  • Page 43 • When using low-profile blades, the red guide (blade rest) (→ "Fig. 32") must first be placed in the blade holder, followed by the blade. Fig. 32 Two magnets (→ "Fig. 33") are attached to the rear side of the guide. These point away from the operator after the guide has been inserted (towards the rear pressure plate). Leica CM1520...
  • Page 44 Daily Use of the Instrument Then insert the blade as described (→ P. 42 – Inserting a high-profile blade). Fig. 33 Removing the blades 1. Fold the anti-roll guide system (→ "Fig. 34-4") to the left – while doing so, hold the lever (→ "Fig. 34-11") (not the adjusting screw of the anti-roll guide), so that the height of the anti-roll guide remains unchanged.
  • Page 45 To do so, follow these steps: 1. Turn the clamping lever (→ "Fig. 37-14") to the rear (counterclockwise) to release, and then shift the blade holder sideways to the desired position. 2. To tighten, turn the clamping lever (→ "Fig. 37-14") to the front (clockwise). Fig. 37 Leica CM1520...
  • Page 46 Daily Use of the Instrument Adjusting the anti-roll guide system You can adjust the height of the anti-roll guide system using the knurled nut (→ "Fig. 38-8"): • If you turn the nut counterclockwise, the anti-roll guide system moves toward the blade. • If you turn the nut clockwise, the anti-roll guide system moves away from the blade.

  • Page 47: Blade Holder Ce-Tc

    All 4 longitudinal edges of the anti-roll glass plate can be used. 7.4.5 Blade holder CE-TC The blade holder CE-TC (→ "Fig. 41") is designed only for disposable carbide metal blades (tungsten carbide - TC65). Handling of this holder is identical to that of the blade holder CE (→ P. 42 – 7.4.4 Blade holder CE). Leica CM1520...

  • Page 48: Knife Holder Cn

    Daily Use of the Instrument Fig. 41 7.4.6 Knife holder CN Inserting the base 1. To insert the blade/knife holder base (→ "Fig. 42-1"), move the lever (→ "Fig. 42-2") counterclockwise to the back. Slide the base onto the t-piece (→ "Fig. 42-3") of the base plate. Note When inserting the blade/knife holder base, overcome the resistance of a spring (located in the foot of the knife holder) by applying a little pressure towards the left.
  • Page 49 The height of the knife blade should match that of the rear clamping jaws. This makes it possible to accurately adjust even frequently resharpened knives up to a height of 25 mm. • Once the correct height is obtained, tighten the wingscrews (→ "Fig. 44-7") in alternating order until both are tight. Leica CM1520...
  • Page 50 Daily Use of the Instrument Fig. 44 • To remove the knife, follow these instructions in reverse order. • Open the wingscrews by turning them counterclockwise and then pull the knife out to the side. Warning • After the knife has been removed from the knife holder, it is stored securely in the knife case (→ "Fig. 45").
  • Page 51 If large specimen discs are to be used with the knife holder (e.g. 50 x 80 mm), it is possible to shift the clamping jaws laterally. The clamping jaws are factory-installed in the knife holder with a clearance of 64 mm. If necessary, both clamping jaws can be set with a clearance of 84 mm. Leica CM1520...
  • Page 52 Daily Use of the Instrument Perform the following steps: 1. Use a No. 4 Allen key to loosen the screw over the clearance angle adjustment (→ "Fig. 47-23") and remove the segment arc (→ "Fig. 47-2") from the blade/knife holder base. Fig. 47 2. Use a No. 4 Allen key to loosen the screws (→ "Fig. 48-4") on the underside of the segment arc.

  • Page 53: Cleaning The Blade And Knife Holders

    (with alcohol). 5. Loosen the blade holder clamping lever (→ "Fig. 50-12") by turning it counterclockwise and pull it out. The blade holder can now be moved sideways and can be taken out of the segment arc. Leica CM1520...
  • Page 54 Daily Use of the Instrument Fig. 50 Knife holder CN • Occasionally oil the moving parts such as the axes (→ "Fig. 51-1") (→ "Fig. 51-2") as well as the slot (→ "Fig. 51-3") with a drop of cryo oil. Fig. 51 Note If several blade/knife holders are cleaned at the same time, the parts must NOT be mixed up! Failure to adhere to this may result in sectioning problems! Version 2.0, Revision K...
  • Page 55 (→ P. 34 – 6.2.6 Setting the section thickness). 7. For section removal, fold the anti-roll guide on the knife and align it with the blade edge. 8. Readjust the anti-roll guide if necessary (→ P. 46 – Adjusting the anti-roll guide system) the blade holders and (→ P. 51 – Clearance angle adjustment) the knife holders. Leica CM1520...

  • Page 56: Temperature Selection Chart (In Minus °C)

    Daily Use of the Instrument Temperature selection chart (in minus °C) Tissue type -10 °C -15 °C -25 °C – -15 °C – -25 °C – -30 °C Adrenal   Bone marrow  Brain  Bladder  Breast - fatty  Breast - little fat  Cartilage  ...

  • Page 57: Defrosting

    To avoid frost formation, always put the proper cover on the quick freeze shelf. Always cover the quick freeze shelf during breaks and overnight. In addition to the programmed automatic defrost, a manual defrost for the cryochamber can also be activated (→ P. 33 – 6.2.5 Manual defrosting of the cryochamber). Leica CM1520...

  • Page 58: Manual Defrosting Of The Quick Freeze Shelf

    Daily Use of the Instrument 7.6.3 Manual defrosting of the quick freeze shelf Warning The quick freeze shelf can become hot during the defrosting process. Therefore, do not touch it! In case of heavy frost formation on the quick freeze shelf, a defrost can be activated manually (→...

  • Page 59: Switching Off The Instrument For A Longer Period

    11. Let the sliding window open to allow the cryochamber to dry and the disinfectant to evaporate. Note Switching off the instrument via the circuit breaker will not affect the programmed values. Before turning the instrument on again, the cryochamber, the microtome and all accessory components must be absolutely dry. Leica CM1520...

  • Page 60: Troubleshooting

    Troubleshooting Troubleshooting Error messages in the display Error messages are displayed on the clock panel as follows EO: XX (→ "Fig. 52"). The following error messages might occur during operation: Fig. 52 Error Cause Remedy Calibration error; possibly defective controller board. Switch the instrument off for 10 seconds and then back on.

  • Page 61: Temperature Control Button

     Clean the air inlet openings (→ "Fig. 56-5"). After eliminating the possible sources of error, push the temperature control button (→ "Fig. 53-1") turn the instrument back on and make it ready to operate. If the instrument does not respond, contact service. Leica CM1520...

  • Page 62: Possible Sources Of Error, Causes And Remedies

    Troubleshooting Possible sources of error, causes and remedies Problem Cause Remedy Frost on cryochamber walls and • The cryostat is exposed to air • Eliminate cause or relocate microtome. currents (open windows, doors, instrument. air conditioning). • Sliding window was open too • Close the sliding window long at a very low cryochamber correctly.
  • Page 63 Anti-roll plate damaged after • Anti-roll plate too high above • Replace anti-roll plate; then lift adjustment. the knife edge. The adjustment it during adjustment. was carried out in the direction • Handle anti-roll plate more of the knife. carefully! Leica CM1520...
  • Page 64 Troubleshooting Problem Cause Remedy Thick/thin sections. • Temperature incorrect for the • Select the correct temperature tissue to be sectioned. and wait until the correct temperature is reached. • Knife profile being used • Use knife with different profile is inappropriate for this (c or d) or switch to disposable specimen.

  • Page 65: Changing The Battery

    If the customer has decided against a service contract, the battery must be replaced after 7 years at the latest. All settings (time, defrost time, section thickness etc.) are lost when the instrument is switched off. There is no danger for the instrument. However, the customer must re-enter the setting values when switching the instrument on for the first time after changing the battery. Leica CM1520...

  • Page 66: Cleaning, Disinfection, Maintenance

    Cleaning, Disinfection, Maintenance Cleaning, Disinfection, Maintenance Cleaning Warning • Wear protective clothing during cleaning or disinfection work (gloves, face mask, lab coat, etc.). • Do not use any solvents (xylene, acetone, etc.) for cleaning or disinfection. • When using detergents and disinfectants, observe the specifications of the respective manufacturer. • Risk of explosion when using alcohol: Provide proper ventilation and ensure the instrument is switched off.

  • Page 67: Maintenance

    To ensure a smooth operation of the instrument over a long period of time we recommend the following: • At least once a year, have the instrument inspected by a qualified service engineer authorized by Leica. • Enter into a service contract at the end of the warranty period. For further information, please contact your local Leica service center. • Clean the instrument DAILY. Weekly: Note Before the following parts can be lubricated, all section waste and deposits must be carefully removed.
  • Page 68 Note Do not carry out any repairs on your own as this will invalidate the warranty. Repairs may only be carried out by qualified service engineers authorized by Leica. Fig. 55 Fig. 56 Version 2.0, Revision K...

  • Page 69: Replacing The Fuses

    1. Unscrew the sleeve of the defective fuse using the screwdriver. 2. Remove the sleeve and fuse. 3. Insert the defined fuse into the sleeve, then use the screwdriver to screw the sleeve back in as far as it will go. Fig. 58 Fuse Function/protection Type Empty Coarse feed T 1.6 A Processor board power supply T 1.0 A Heaters T 6.25 A Leica CM1520...

  • Page 70: Removing The Sliding Window

    6. Connect the instrument to the power supply again and switch it on. Fig. 59 9.3.4 Replacing the LED illumination The LED illumination is designed for maximum life span. In case of damage, please contact Leica customer service to arrange replacement. For further details, see (→ P. 97 – 11. Warranty and Service). Version 2.0, Revision K...

  • Page 71: Ordering Information, Components And Consumables

    Knife support for knife holder CN, for short knives 14 0419 19426 Knife support for knife holder CN, for long knives 14 0419 19427 Disposable blades Leica TC-65 disposable blades, 5 pcs. 14 0216 26379 Leica high-profile disposable blades, Type 818, 1 pack of 50, 14 0358 38926 Leica high-profile disposable blades, Type 818, 10 packs of 50...
  • Page 72 Ordering Information, Components and Consumables Description Order No. Embedding well bar, 3 x 30 mm 14 0201 39122 Specimen stage, rectangular, 28 mm 14 0201 39123 Specimen stage, rectangular, 36 mm 14 0201 39124 Heat extractor 14 0201 39125 Container for specimen stages 14 0201 39126 Dispensing slides for Dr.
  • Page 73 Knife/blade holder base, for knife holder CN, blade holder CE, CE-TC, premium blade holder Order No. 14 0491 47875 Fig. 60 Premium blade holder, assembly, for high-profile and low-profile blades, with lateral movement, glass anti-roll guide and palm rest Order No. 14 0491 48023 Fig. 61 Leica CM1520...
  • Page 74 Ordering Information, Components and Consumables Knife holder CN, for standard microtome knives or magnetic blade rails. Clearance angle adjustment and knife height adjustment. Backrests for long and short knives. Anti-roll guide and adjustable safety guard. Order No. 14 0477 42358 Fig. 62 Blade holder CE, universal (high-profile and low-profile disposable...
  • Page 75 Order No. 14 0800 37740 Anti-static kit for blade holder CE, low-profile Order No. 14 0800 37739 Fig. 67 Backrest for knife holder CN, for short knives Order No. 14 0419 19426 Fig. 68 Backrest for knife holder CN, for long knives Order No. 14 0419 19427 Leica CM1520...
  • Page 76 Ordering Information, Components and Consumables Disposable blades Leica TC-65, Leica TC-65 microtome, disposable blade system for sectioning hard specimen materials. The Leica TC-65 tungsten carbide disposable blades were specially developed for the requirements in labs where hard, blunt materials are routinely sectioned.
  • Page 77 Ordering Information, Components and Consumables Knife, 16 cm, steel, profile c, (flat on both sides, for wax and frozen sections). Serial number of the knife (→ "Fig. 72-1") Note: including knife case 14 0213 11140 Order No. 14 0216 07100 Fig. 72 Knife, 16 cm, tungsten carbide, profile c, Note: including knife case 14 0213 11140 Order No. 14 0216 04206 Fig. 73 Leica CM1520...
  • Page 78 Ordering Information, Components and Consumables Knife, 16 cm, steel, profile d, Note: including knife case 14 0213 11140 Order No. 14 0216 07132 Knife, 16 cm tungsten carbide, profile d, Note: including knife case 14 0213 11140 Serial number of the knife (→ "Fig. 74-1") Order No. 14 0216 04813 Fig. 74 Variable knife case, For 1 to 2 knives from 10 to 16 cm in length Order No.
  • Page 79 - 4 specimen stages, large - 4 heat extractors - 1 container for specimen stages - 16 dispensing slides Fig. 77 - 1 sectioning board/freezing stage - 1 heat extractor, elevated - 1 embedding forceps, angled Order No. 14 0201 39115 Leica CM1520...
  • Page 80 Ordering Information, Components and Consumables Embedding well bar set with small recesses consisting of: - 1 embedding well bar, 18 mm recesses - 4 specimen stages, small - 2 heat extractors - 8 dispensing slides Order No. 14 0201 39116 Embedding well bar set with medium-sized recesses consisting of: Fig. 78...
  • Page 81 - 28 mm Pack of 1. Order No. 14 0201 39123 Specimen stage, rectangular, stainless steel, size - 36 mm Fig. 81 Pack of 1. Order No. 14 0201 39124 Heat extractor Order No. 14 0201 39125 Fig. 82 Leica CM1520...
  • Page 82 Ordering Information, Components and Consumables Container for specimen stages Order No. 14 0201 39126 Fig. 83 Dispensing slides for Dr. Peters' cryoembedding system Pack of 8. Order No. 14 0201 39127 Fig. 84 Version 2.0, Revision K...
  • Page 83 Toolset for cryostats, consisting of: • 1 brush, narrow 14 0183 28642 • 1 Leica brush with magnet 14 0183 40426 • 1 Allen key with handle, No. 5 14 0194 04760 • 1 Allen key, No. 2.5 14 0222 04137 • 1 Allen key, No.
  • Page 84 Ordering Information, Components and Consumables Easy Dip, specimen slide holder, for 12 specimen slides, gray Pack of 6 Order No. 14 0712 40161 Fig. 87 Easy Dip, staining container Color: white Pack of 6 Order No. 14 0712 40150 Color: pink Fig. 88 Pack of 6 Order No.
  • Page 85 25 mm Order No. 14 0416 19275 30 mm Order No. 14 0370 08587 40 mm Order No. 14 0370 08637 55 mm Order No. 14 0419 26491 50 x 80 mm Order No. 14 0419 26750 Fig. 89 Leica CM1520...
  • Page 86 Ordering Information, Components and Consumables Fig. 90 Heat extractor, stationary (optional) Brush shelf Freeze shelf, 10 positions Specimen head, orientable Shelf, movable (optional) Waste tray Blade holder CE with anti-roll guide - also Storage space functions as a safety guard (optional) Version 2.0, Revision K...
  • Page 87 (13 slots) Order No. 14 0491 47786 Fig. 91 Transfer block for specimen discs, small (5 slots) Order No. 14 0491 47787 Fig. 92 Large transfer blocks (→ "Fig. 91") and small transfer blocks (→ "Fig. 92") can be combined (→ "Fig. 93"). Fig. 93 Leica CM1520...
  • Page 88 Ordering Information, Components and Consumables Heat extractor mobile (→ P. 94 – 10.2 Mobile heat extractor - application) Order No. 14 0443 26836 Fig. 94 Thermal block for easy removal of frozen tissue from the specimen disc (→ P. 94 – 10.3 Cold extraction - using the Thermal block). Order No. 14 0398 18542 Fig. 95 Adapter for Miles Tissue Tek specimen discs.
  • Page 89 Glass - 70 mm, for special application, consisting • Glass insert 70 mm • Metal frame for exchangeable glass inserts CE • 50 µm spacer Recommended section thickness up to 4 µm For blade holder CE: (14 0491 47873, 14 0419 33992) Fig. 99 Order No. 14 0419 37258 Leica CM1520...
  • Page 90 Ordering Information, Components and Consumables Anti-roll guide system CE Glass - 70 mm, for special application, consisting • Glass insert 70 mm • Metal frame for exchangeable glass inserts CE • 150 µm spacer Recommended section thickness over 50 µm For blade holder CE: (14 0491 47873, 14 0419 33992) Order No.
  • Page 91 Blue, 9 x 118 ml Order No. 380 1481 Fig. 104 Both media may not be available in your country. Please contact your local distributor. Embedding medium for cryosectioning Tissue Freezing Medium, 125 ml Order No. 14 0201 08926 Fig. 105 Leica CM1520...
  • Page 92 Ordering Information, Components and Consumables Cryo oil 250 ml bottle Order No. 14 0336 06100 Fig. 106 Cut-resistant safety gloves 1 pair, size S Order No. 14 0340 40859 Cut-resistant safety gloves 1 pair, size M Fig. 107 Order No. 14 0340 29011 Version 2.0, Revision K...
  • Page 93 Low-temperature buffer for heat extractor (parking station) (→ "Fig. 108-1") Order No. 14 0471 30793 Fig. 108 Storage system, movable for installation in the front part of the cryostat for cooled storage of preparation aids. Order No. 14 0491 46750 Fig. 109 Leica CM1520...

  • Page 94: Mobile Heat Extractor - Application

    Ordering Information, Components and Consumables 10.2 Mobile heat extractor - application Specimen freezing with the freezing shelf can be accelerated by the additional use of a heat extractor. 1. Store the heat extractor in the cryochamber. 2. Hold it on the specimen surface to accelerate freezing. 3.

  • Page 95: Stationary Heat Extractor (Optional) - Application

    • Move the heat extractor from the parking station and lower it directly onto the surface of the specimen until it is completly frozen. • After the specimen is frozen, move the heat extractor into resting position at the parking station (→ "Fig. 111-4"). Fig. 111 Leica CM1520...

  • Page 96: Installing The Shelf (Movable) (Optional)

    Ordering Information, Components and Consumables 10.5 Installing the shelf (movable) (optional) • Attach the rod for the shelf to the inner front side of the cryostat housing using the provided screws (→ "Fig. 112-1") and the No. 3 Allen key, then attach the caps (→ "Fig. 112-3"). (The rear side of the movable shelf has white plastic screws (→ "Fig. 112-2") that prevent the interior of the...

  • Page 97: Warranty And Service

    11. Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. The scope of the warranty is based on the content of the concluded agreement. The warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall apply exclusively.

  • Page 98: Decontamination Certificate

    Decontamination Certificate 12. Decontamination Certificate Any product that is to be returned to Leica Biosystems or serviced on site must be properly cleaned and decontaminated. The associated decontamination certificate template can be found on our website www. LeicaBiosystems.com in the product menu. This template must be used to record all required data. If a product is returned, a copy of the completed and signed decontamination certificate must either be enclosed or handed over to a service technician. The user shall be responsible for products that are returned without a completed decontamination certificate or with a missing decontamination certificate.
  • Page 100 Leica Biosystems Nussloch GmbH Heidelberger Str. 17 - 19 69226 Nussloch Germany Tel.: +49 - (0) 6224 - 143 0 Fax: +49 - (0) 6224 - 143 268 Web: www.LeicaBiosystems.com...

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