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Recommended settings of the fluorifier disc for SP FLIM detection:
Excitation laser line
1
405
2
405+470
3
405+ 640
4
470
5
470+640
6
640
7
MP
8
WLL
Table 19:
Recommended settings for Fluorifier disc for SP FLIM
As long as the Fluorifier Disc Auto Select is active in the dialog window, the position of the
Fluorifier disc is automatically set. It can be changed by the user if Auto Select is disabled.
To do so, proceed as follows:
1. In Beam Path Settings, click on the Fluorifier Disc button (Figure 125).
Figure 125:
Fluorifier Disc button in Beam Path Settings
The following dialog box opens:
Figure 126:
Fluorifier Disc Settings
Recommended setting of the fluorifier disc
Barrier filter 405/640
Barrier filter 405/470
Barrier filter 405/640
Barrier filter 405/470
Barrier filter 470/640
Barrier filter 405/640 or 470/640
SP680 or SP700
Depending on the selected white light laser wavelength,
there are barrier filters for:
488, 514, 561, 594, or 633
FLIM Data Acquisition
143
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