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LAS AF
• Step Measurements: Here, you can define multiple FCS measuring points in the
previously acquired xy or xz image or xyz or xzy stack, then carry out consecutive and
automatic FCS measurements defined in the Setup FCS step. The list of measuring points
can be saved and reloaded.
In each step, different tabs are shown in the tab area:
• On the "Workflow" tab you will find a suggested workflow and the functions associated
with it.
• On the "Experiments" tab, the image data are stored.
• On the Acquisition tab, you can configure the settings for image acquisition, such as
scan speed, zoom or pinhole diameter, or you can define a series of FCS measurements.
In all steps of the Wizard, you can access continuous scanning or acquire a single image or
z or y-stack at any time with "Live", Capture image and Start. The conditions for image
acquisition that you defined in Setup Imaging are used for this.
Then, if you carry out the FCS measurement in step Setup FCS and do not move the
crosshair, the scanner is simply parked in the center of the image.
After the completion of the FCS measurements, you can close the Wizard by selecting the
operating mode TCS SP8 (Figure 91).
Figure 91:
112
FCS Measurements in Solutions
Even when carrying out FCS measurements in solutions, you have to
acquire an xy image before the first FCS measurement. This is necessary
so that the scanner can become oriented in the scan field. The
information content of this image is not relevant here.
Closing the Wizard
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