Related Manuals for Leica HistoCore MULTICUT
Summary of Contents for Leica HistoCore MULTICUT
- Page 1 HistoCore MULTICUT Rotary Microtome Instructions for Use English Order No.: 14 0518 80101 - Revision C Always keep this manual with the instrument. Read carefully before working with the instrument.
- Page 3 These are determined only by the contract provisions agreed between ourselves and our customers. Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products.
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Page 4: Table Of Contents
Table of Contents Important Information ..........................Symbols used in the text and their meanings ..................... 7 Instrument type ..............................9 Intended use ..............................9 Qualification of personnel ..........................9 Safety ................................Safety notes ..............................10 Warnings ................................. 10 2.2.1 Markings on the instrument itself ....................... 11 2.2.2 Transport and installation .......................... - Page 5 Troubleshooting ............................Error codes ..............................85 Possible faults ..............................86 Instrument malfunctions..........................88 Malfunctions of the two-in-one blade holder E ..................89 7.4.1 Replacing the pressure plate........................89 7.4.2 Mounting the clamping top onto the segment arc ................... 90 HistoCore MULTICUT...
- Page 6 Table of Contents The setting of the clamping system of the clamp mount on the base plate ........90 Cleaning and Maintenance ........................Cleaning the instrument..........................91 Maintenance ..............................94 8.2.1 Replacing fuses .............................. 94 Maintenance instructions ..........................95 Lubricating the instrument ........................... 96 Warranty and Service ..........................
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Page 7: Important Information
Symbol for labeling electrical and electronic equipment in accordance with Section 7 of the German Electrical and Electronic Equipment Act (ElektroG). ElektroG is the law on the bringing into circulation, return and environmentally compatible disposal of electrical and electronic equipment. HistoCore MULTICUT... - Page 8 Important Information The CSA test mark means that a product has been tested and fulfills the applicable safety and/or performance standards, including the relevant standards defined or administered by the American National Standards Institute (ANSI), Underwriters Laboratories (UL), the Canadian Standards Association (CSA), the National Sanitation Foundation International (NSF) and others.
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Page 9: Instrument Type
Intended use The HistoCore MULTICUT is a semi-motorized (motorized specimen feeding), manually operated rotation microtome specifically designed for creating thin sections of human tissue specimens of varying hardness for histological medical diagnosis, e.g. cancer diagnosis. It is intended for sectioning soft and hard human specimens, as long as they are suitable for being cut manually. -
Page 10: Safety
Instructions for Use. The safety and caution notes in this chapter must be observed at all times. Be sure to read these notes even if you are already familiar with the operation and use of other Leica Biosystems products. -
Page 11: Markings On The Instrument Itself
Ground needs to be vibration-free as far as possible. • Always make sure that all 4 feet are positioned completely on the laboratory table. • If the instrument has been potentially moved by other persons (for example for servicing) always double-check correct positioning. HistoCore MULTICUT... - Page 12 Safety Warning Condensation inside the instrument due to exposure to extreme differences in temperature and high air humidity. Damage of the instrument. • Always make sure that for both storage and operation appropriate climate conditions are met. See the section of Technical data (→...
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Page 13: Operating The Instrument
Serious injury of persons and/or property damage including specimen damage. • Never remove or modify any protective devices of the instrument or any accessories. Only service personnel qualified by Leica Biosystems may repair the instrument and access the instrument's internal components. - Page 14 Safety Warning Personal protective equipment is not used. Injury of persons. • When working with microtomes, personal safety precautions must always be taken. It is mandatory to wear work safety shoes, safety gloves, a mask and safety goggles. Warning Knives or blades are handled and/or disposed in an inappropriate manner. Serious injury of persons due to contact with extremely sharp knife/blade.
- Page 15 Working with the instrument and brittle specimen without appropriate protective clothes. Serious injury of persons by splinters while cutting brittle specimen. • Always wear appropriate protective clothes (including safety goggles) and work especially carefully when cutting brittle specimen. HistoCore MULTICUT...
- Page 16 • Make sure that all prerequisites for appropriate sectioning are met. Refer to the troubleshooting section of these Instructions for Use for further details. • If you do not have sufficient knowledge in regards of troubleshooting insufficient sectioning results consult persons who do have that knowledge, for example, application experts of Leica Biosystems. Warning Wheel turn direction of electric coarse feed incorrectly selected.
- Page 17 • If instrument cannot set back into operation immediately, make sure that the specimen is stored in an appropriate manner to avoid any damage to it. • If required, contact Leica Biosystems service representative. Caution After the knife/blade holder is laterally displaced, the specimen is not retracted and retrimmed.
- Page 18 Safety Caution The feather key is lost when mounting the handwheel. The instrument cannot be used, and it may cause the delay of diagnosis. • Before assembling the handwheel, check the feather key and make sure that it is in the handwheel shaft.
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Page 19: Cleaning And Maintenance
• Ensure that liquids do not enter the interior of the instrument during operation and maintenance. Spray proper solvents or cleaners on a cleaning pad and not directly on the instrument to prevent liquids from entering. If liquids go into interior of instrument, contact Leica Biosystems service. Warning Wiping the knife in the wrong direction during cleaning. -
Page 20: Integrated Protective Devices
Serious injury of persons and/or property damage including specimen damage. • Never remove or modify any protective devices of the instrument or any accessories. Only service personnel qualified by Leica Biosystems may repair the instrument and access the instrument's internal components. -
Page 21: Locking The Handwheel
When using both brake systems at the same time, always move the handwheel brake lever (→ "Fig. 1-3") to position (→ "Fig. 1-5") first. Otherwise, it may be not be possible to release the handwheel locking mechanism (→ "Fig. 1-2"). HistoCore MULTICUT... -
Page 22: Safety Guard On The Knife/Blade Holder
Safety 2.3.2 Safety guard on the knife/blade holder Each knife holder is equipped with a tightly mounted safety guard (→ "Fig. 3-1") (→ "Fig. 4-1"). This makes it possible to cover completely the cutting edge in every knife or blade position. Knife holder N The safety guard (→... -
Page 23: Instrument Components And Specifications
Handwheel brake lever Separate control panel 10 Object head with orientation and UCC Microtome base 11 Handwheel Blade holder base 12 Handwheel locking mechanism Two-in-one blade holder E 13 Instrument control panel Clamping lever of the knife/blade holder base HistoCore MULTICUT... -
Page 24: Instrument Specifications
Instrument Components and Specifications Rear view Fig. 6 Magnet Power supply Voltage selector switch with fuse Vents Main switch Connection for service Instrument specifications Basic instrument with separate control panel, programmable retraction with ON/OFF selection, unique ergonomically positioned electronical lateral coarse feed wheel with user selectable turn directions, without specimen orientation or additional accessories. -
Page 25: Technical Data
477 mm coarse feed wheel) Depth (including the section waste tray) 620 mm Height (without the top tray) 295 mm Weight (without accessories) Approx. 31 kg Volume of the section waste tray 1400 ml Control panel Width 94 mm HistoCore MULTICUT... - Page 26 Instrument Components and Specifications Dimensions and weights Depth 193 mm Height 50 mm Height (in inclined position) 81 mm Weight (net) approx. 0.5 kg Microtome Sectioning thickness setting Section thickness setting range 0.50 - 100 µm Setting values • From 0.5 - 5.0 µm in 0.5 µm increments •...
- Page 27 Specimen orientation with zero postion • Horizontal rotation: ± 8° • Vertical rotation: ± 8° Two-in-one blade holder • Lateral displacement function • 3 positions East-west movement • Movement of blade holder base: • North-south: ± 24 mm HistoCore MULTICUT...
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Page 28: Setting Up The Instrument
Universal cassette clamp 14 0502 37999 Blade holder base 14 0502 55546 2-in-1 Blade holder E 14 0502 54497 HistoCore MULTICUT basic instrument includes the following delivery list. Quantity Part description Order No. HistoCore MULTICUT basic instrument 14 0518 56372... - Page 29 The accessories ordered are included in a separate box. Please check all delivered parts against the packing list and against your order to verify whether the delivery is complete. Should you find any discrepancies, please contact your Leica Biosystems sales office without delay.
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Page 30: Unpacking And Installation
Setting up the instrument Unpacking and installation Warning Accessories/instrument falling out of the package during unpacking. Serious injury of persons and/or property damage. • Be careful when unpacking the instrument. • Once unpacked, the instrument must be moved only in an upright position. •... - Page 31 (→ "Fig. 7-1"). • Remove the carton lid (→ "Fig. 7-2"). Fig. 7 • Take out the accessory carton (optional accessories) (→ "Fig. 8-3") and the cartons (→ "Fig. 8-4") for the standard scope of delivery. Fig. 8 HistoCore MULTICUT...
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Page 32: Assembling The Handwheel
Setting up the instrument • Take out the fixing module (→ "Fig. 9-5"). To do so, hold it by the top edge of the module and in the recessed grip (→ "Fig. 9-6") and pull it out by pulling upwards. •... -
Page 33: Electrical Connections
• Do not use an extension cord. 4.5.1 Checking the voltage The HistoCore MULTICUT can be connected to various electrical power grids (depending on voltage and frequency). New instruments are factory-set to 230 volts. This is documented by a yellow label (230 VOLT) on the rear of the instrument, which covers the power switch and power socket. -
Page 34: Connecting The Power Supply
Setting up the instrument Warning Wrong voltage of power supply selected at the voltage selector. Instrument damage, delay of specimen processing. • Assure to select the voltage fitting to your mains voltage before plugging in the instrument. • When changing the voltage selector setting, the instrument must be disconnected from the mains. Fig. -
Page 35: Connecting The Control Panel
LED display. This display disappears after 2 seconds and "00.00" is displayed. After the microtome is switched on, the display fields and LEDs of all activated functions are lit up on the instrument's control panel and display field. HistoCore MULTICUT... - Page 36 Setting up the instrument The three-digit LED display indicates the last value set for the section thickness or trimming thickness, depending on which setting was last activated. This is indicated simultaneously on the control panel and microtome. The LED of the active mode (here the section thickness) is illuminated in green.
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Page 37: Operation
• If instrument cannot set back into operation immediately, make sure that the specimen is stored in an appropriate manner to avoid any damage to it. • If required, contact Leica Biosystems service representative. 5.1.1 Instrument control panel Fig. 14 LED - Lights up during specimen retraction. -
Page 38: Separate Control Panel
Operation LED - LOCK Lights up when the handwheel lock is active. Green LED - Lights up when the trimming mode is active. TRIM Green LED - SECT Lights up when the sectioning mode is active. Green LED Section thickness sum - shows the sum of all sections. Green LED Section counter - displays the number of all sections. -
Page 39: Display And Control Elements
TRIM. SECT In the SECT display, the sectioning thickness in the range from 0.50 to 100.0 µm is shown, and in the display, the trimming thickness between TRIM 1.0 and 600 µm is displayed. HistoCore MULTICUT... - Page 40 Operation Setting the section thickness/trimming thickness Adjust the settings using these two buttons on the separate control panel. Section thickness setting range: 0.50 µm - 100 µm Setting values: from 0.5 µm - 5.0 µm in 0.5 µm increments from 5.0 µm - 20.0 µm in 1.0 µm increments from 20.0 µm - 60.0 µm in 5.0 µm increments from 60.0 µm -100.0 µm in 10.0 µm increments Trimming thickness setting range: 1 - 600 µm...
- Page 41 The travel continues as long as the button is held pressed. • During the forwards movement, the yellow LED (→ "Fig. 18-4") in the button flashes. When the front end position is reached, an acoustic signal is heard and the LED stops flashing and remains lit. HistoCore MULTICUT...
- Page 42 Operation One memory position Set one memory position using the MEMO button (→ "Fig. 19-1") on the separate control panel. To set one memory position, move the object head to the desired position, and press the MEMO button. A short beep is heard; the three-digit display indicates (→...
- Page 43 The retraction value can be selected in 5 µm increments between 5 and 100 µm. Specimen retraction is set to 10 µm at the factory. Specimen retraction can be disabled if required. The selected setting is maintained when the instrument is turned off. HistoCore MULTICUT...
- Page 44 Operation Configuring the retraction settings • To call up the retraction settings, press the MENU MODE (→ "Fig. 21-1") (→ "Fig. 21-2") buttons simultaneously. CLEAR • The current set value is displayed as a three-digit number in the four-digit display, e.g. 025 = 25 µm (→...
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Page 45: Electronic Coarse Feed Wheel
The settings of the electronic coarse feed wheel Fig. 26 rotation is shown for about 4 seconds on the three-digit display when the instrument switches on. The factory-set rotation direction of the electronic coarse feed wheel is clockwise. HistoCore MULTICUT... -
Page 46: Fine Directional Specimen Holder Fixture
Operation 5.1.5 Fine directional specimen holder fixture In the quick clamping system of the directional specimen holder fixture, all specimen clamps available as optional accessories can be used. Fig. 27 The specimen orientation allows for simple position correction of the specimen surface when the specimen is clamped into place. -
Page 47: Fine Adjustment Of The Force Balance
• If fine adjustment is not properly set, do not work with the instrument but perform a readjustment. • Especially after changing accessories at the object head, perform a fine adjustment of the force balance immediately. HistoCore MULTICUT... -
Page 48: Inserting The Two-In-One Blade Holder E
Operation The force balance is adjusted using the screw (→ "Fig. 29-1"), which can be accessed by removing the section waste tray on the bottom of the base plate of the microtome. Use the Allen key No. 5 (with handle) for the adjustment. •... -
Page 49: Inserting The Two-In-One Blade Holder E
1. Loosen the screw (→ "Fig. 32-3") using an Allen key No. 4 (→ "Fig. 32-6") until the two-in-one blade holder E (→ "Fig. 32-2") can be moved. HistoCore MULTICUT... -
Page 50: Inserting The Universal Cassette Clamp
Operation 2. Move the two-in-one blade holder E until the index mark of the desired clearance angle coincides with the reference line on the blade holder base. Enlarged detail (→ "Fig. 32") shows a clearance angle setting of 5°. Tips The recommended clearance angle setting for the two-in-one blade holder E is approx. -
Page 51: Clamping The Specimen
Serious injury of persons due to contact with extremely sharp knives and/or blades. • Do not clamp two knives/blades on the knife holder/blade holder. Install the knife/blade in the center of the knife/blade holder. Knives/Blades must not exceed the edge of the knife/blade holder. HistoCore MULTICUT... - Page 52 Operation Inserting high-profile blades Fig. 34 Fig. 35 1. Bring the safety guard (→ "Fig. 34-1") down. 2. To insert the blade, rotate the right clamping lever (→ "Fig. 34-2") counterclockwise. 3. Carefully push in the blade from above or from the side. Make sure that the blade is positioned in the center and, most importantly, parallel to the upper edge of the pressure plate(→...
- Page 53 • Never place a knife anywhere with the cutting edge facing upwards and never try to catch a falling knife. • Always cover the edge of the knife/blade with the safety guard before clamping the specimen. HistoCore MULTICUT...
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Page 54: Trimming The Specimen
Operation Detailed descriptions for inserting the knife into the individual knife holders are provided in (→ P. 59 – 6. Optional Accessories). Trimming the specimen Warning Wheel turn direction of electric coarse feed incorrectly selected. Specimen damage. • Always assure that you have selected the correct turn direction of the coarse feed wheel before turning the wheel. -
Page 55: Sectioning
• Make sure that all prerequisites for appropriate sectioning are met. Refer to the troubleshooting section of these Instructions for Use for further details. • If you do not have sufficient knowledge in regards of troubleshooting insufficient sectioning results consult persons who do have that knowledge, for example, application experts of Leica Biosystems. Warning Turning the handwheel counterclockwise. - Page 56 Operation Warning The rotation speed of the handwheel does not suit the hardness of the specimen. Instrument damage and possibly specimen damage. • The rotation speed of the handwheel must be adapted to suit the hardness of the specimen. For harder specimens, use a slower speed.
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Page 57: Changing The Specimen Or Interrupting Sectioning
Fig. 43 5.10 Finishing the daily routine Warning Section waste tray falling down after it has been detached. Injury of persons. • Work especially carefully when detaching the section waste tray and put it in a safe place. HistoCore MULTICUT... - Page 58 Operation Warning Knife/blade was not removed when knife/blade holder was uninstalled from the microtome. Serious injury of persons due to contact with extremely sharp knife/blade. • Before uninstalling the knife/blade holder from the microtome always make sure to detach the knife/ blade wearing cut-resistant gloves and store the knife/blade in a safe place.
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Page 59: Optional Accessories
(→ "Fig. 44-2") with an Allen key No. 3. • Next, insert the screw (→ "Fig. 44-1") from the side and briefly tighten it with an Allen key No. 4. Fig. 44 HistoCore MULTICUT... -
Page 60: Directional Fixture For Specimen Clamps
Optional Accessories 6.1.2 Directional fixture for specimen clamps • Loosen the eccentric bolt (→ "Fig. 45-1") turning it counterclockwise. • Completely unscrew the thrust piece (→ "Fig. 45-2") with a flat-tip screwdriver and pull it out with spring (→ "Fig. 45-3") and pin (→... -
Page 61: Quick Clamping System
All specimen clamps available as accessories can be integrated into the fine directional, or directional, or non-directional specimen holder fixtures. For how to install the specimen clamps and holders to the quick clamping system, please refer to (→ P. 50 – 5.4 Inserting the universal cassette clamp). HistoCore MULTICUT... -
Page 62: Standard Specimen Clamp
Instructions for Use for further details. • If you do not have sufficient knowledge in regards of troubleshooting insufficient sectioning results consult persons who do have that knowledge, for example, application experts of Leica Biosystems. 6.2.2 Universal cassette clamp Surgipath cassettes with minimum dimensions of 39.8 x 28 mm and maximum dimensions of 40.9 x... - Page 63 • Make sure that all prerequisites for appropriate sectioning are met. Refer to the troubleshooting section of these Instructions for Use for further details. • If you do not have sufficient knowledge in regards of troubleshooting insufficient sectioning results consult persons who do have that knowledge, for example, application experts of Leica Biosystems. Warning The remaining broken edge of cassette lid may lead to poor section quality due to insecure clamping.
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Page 64: Super Cassette Clamp
Optional Accessories 6.2.3 Super cassette clamp Fig. 50 Assembly of the super cassette clamp Tips Remove the rubber ring (→ "Fig. 50-5") only after having attached the rigid fixture for specimen clamps on the object head. The super cassette clamp should preferably be used with the rigid fixture for specimen clamps. To do so, proceed as follows: •... -
Page 65: Knife Holder Base And Knife Holder
E can occur. Clamping lever for the blade (→ "Fig. 52-8") is at the right, clamping lever for the lateral displacement (→ "Fig. 52-3") is at the left. HistoCore MULTICUT... -
Page 66: Knife Holder Base, Without Lateral Displacement Feature
Optional Accessories Lateral displacement The lateral movement feature of the two-in-one blade holder E enables the use of the entire length of the blade by moving the clamping top laterally. Three predefined stop positions (left, center, right) that correspond to the width of a standard cassette may be used if desired. -
Page 67: Knife Holder N
The knife holder N is appropriate for standard steel knives, profile c and d, up to 16 cm long. The integrated height adjustment feature allows you to also use knives that have been resharpened numerous times. • Knife holder N (→ "Fig. 57"): For holding conventional knives up to 16 cm in length. Fig. 57 HistoCore MULTICUT... - Page 68 Optional Accessories Mounting the knife support bar • Push the safety guard (→ "Fig. 57-1") to the center. • Set the knife support bar (→ "Fig. 57-2") onto the height adjustment screws. The flat ends of the height adjustment screws must be located in the slots at each end of the knife support bar. Warning The knife/blade is installed before the knife/blade holder and the knife/blade holder base are installed on the instrument.
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Page 69: Section Waste Tray
Backlighting Tips The backlighting cannot be used with the blade holder base of the two-in-one blade holder E. To use with backlighting on HistoCore MULTICUT, the external power supply unit (Order No.: 14038631352 ) must be ordered separately. HistoCore MULTICUT... -
Page 70: Top Tray
Optional Accessories • Remove the two screws (→ "Fig. 60-1") using a slotted screwdriver and then remove the cover plate (→ "Fig. 60-2"). • Insert the backlighting (→ "Fig. 60-3") in the recess at the rear of the knife holder base. •... - Page 71 (→ "Fig. 63-4") support plate, depending on the microtome to be used. Attach the support plate to the baseplate with the included 4 countersunk screws (→ "Fig. 63-7") using the Allen key No. 3 (→ "Fig. 63-6"). Fig. 64 HistoCore MULTICUT...
- Page 72 Optional Accessories • Attach vertical column. Insert the cap screw (→ "Fig. 65-12") into the hole of the baseplate from below. Place the lock washer (→ "Fig. 65-13") on the cap screw from above. Thread Fig. 65 the silver vertical column (→...
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Page 73: Magnifying Lens, Led Illumination
LED 1000 Hi-Power spots, 2-arms, into the sockets (→ "Fig. 69-2") of the high-power spot controller (→ "Fig. 69-3"). Connect the power adapter (→ "Fig. 69-4") of the LED 1000 control unit to the high-power spot HistoCore MULTICUT... - Page 74 Optional Accessories controller, then connect to the power supply. Make sure to choose a correct plug from the provided ones (→ "Fig. 69-5") for the power adapter for your region. • Press the button (→ "Fig. 69-7") on the high-power spot controller to turn on or turn off the two spot lights (→...
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Page 75: Additional Accessories
(80 x 8 x 0.25 mm), silver, with a water trough Quick clamp system with ability to shift the clamping top laterally 3 click-stop positions make it easier to utilize the entire blade length Color-coded, foldaway safety guard • Order No.: 14 0502 38961 Fig. 72 HistoCore MULTICUT... - Page 76 • Order No.: 14 0502 55546 Fig. 73 Two-in-one blade holder E For high and low profile disposable blades For HistoCore MULTICUT Ability to shift the clamping top laterally 3 click-stop positions make it easier to utilize the entire blade length The pressure plate is replaceable.
- Page 77 Note: Knife case 14 0213 11140 included • Order No.: 14 0216 07132 Fig. 77 Knife case Variable knife case (plastic), for 1 or 2 knives: 10 - 16 cm long • Order No.: 14 0213 11140 Fig. 78 HistoCore MULTICUT...
- Page 78 Optional Accessories Universal cassette clamp with adapter, silver For use with standard cassettes with dimensions that are 39.8 x 28 x 5.8 mm at minimum and 40.9 x 28.8 x 6.2 mm at maximum. • Order No.: 14 0502 37999 Fig.
- Page 79 For specimen holder for use with the fine- directional fixture for specimen clamps with zero point indicators 14 0502 37717 or the directional fixture for specimen clamps 14 0502 38949 • Order No.: 14 0502 37718 Fig. 85 HistoCore MULTICUT...
- Page 80 Optional Accessories Super cassette clamp With adapter, silver Note: Use only together with non-orientable specimen holder fixture 14 0502 38160, which has to be ordered separately. Backlighting 14 0502 38719 cannot be used in connection with a Super cassette clamp. We recommend use with Surgipath Super cassettes and covers, white (VSP 59060B-BX, VSP 59060-CS) and Super metal embedding molds...
- Page 81 For assembly on microscope carriers (14 0502 40580), 2x magnification Note: Magnifier includes an adapter for holding the optional LED illumination • Order No.: 14 0502 42790 Fig. 88 Universal microscope carrier Universal assembly • Order No.: 14 0502 40580 Fig. 89 HistoCore MULTICUT...
- Page 82 Optional Accessories Backlighting Note: For HistoCore BIOCUT and MULTICUT only together with external power supply 14 0500 31244. Backlighting cannot be used in connection with a Super cassette clamp 14 0502 38967. • Order No.: 14 0502 38719 Fig. 90 External power supply unit For use with backlighting 14 0502 38719, for rotary microtome series HistoCore BIOCUT and...
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Page 83: Ordering Information
Cut resistant safety gloves, size S, 250±20mm • Order No.: 14 0340 40859 Fig. 95 6.10 Ordering information Knife holder base 14 0502 37962 Knife holder N 14 0502 37993 Knife holder E w/trough low-profile 14 0502 38961 Blade holder base 14 0502 55546 HistoCore MULTICUT... - Page 84 Optional Accessories 2-in-1 blade holder E 14 0502 54497 Disposable blades – low-profile, 1 pack of 50 (80 x 8 x 0.25 mm) 14 0358 38925 Disposable blades – low-profile, 10 packs of 50 14 0358 38382 Disposable blades – high-profile, 1 pack of 50 (80 x 14 x 0.35 mm) 14 0358 38926 Disposable blades –...
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Page 85: Troubleshooting
2.Check all setting values to see if the previously entered values are still correct. The values may have to be reentered. 3.If the error code appears repeatedly, call Leica Biosystems service. Error code Object head feeding 1.Restart the instrument and check displayed; a two-... -
Page 86: Possible Faults
Troubleshooting Possible faults Problem Possible cause Corrective action Thick/thin sections • The blade, knife holder or • Reclamp the blade, knife orientation is not clamped holders or orientation. The sections alternate between properly. being thick and thin, or there • The specimen is not clamped •... - Page 87 Leica Biosystems, or the specimen is torn out of the cassette may become the embedding. In extreme deformed or other clamping cases, there are no sections problems may arise.
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Page 88: Instrument Malfunctions
The following is a list of the most common problems which can arise while working with the instrument, along with possible causes and troubleshooting procedures. If the malfunction cannot be remedied with any of the options listed in the table, or the problem occurs repeatedly, inform Leica Biosystems Service Support immediately. -
Page 89: Malfunctions Of The Two-In-One Blade Holder E
Make sure that the insert of the clamping top is installed as shown (→ "Fig. 97-5") in case it falls out. If it is installed wrongly, the pressure plate cannot be clamped. 5. Install a new pressure plate. HistoCore MULTICUT... -
Page 90: Mounting The Clamping Top Onto The Segment Arc
Troubleshooting 6. Insert the bolt (→ "Fig. 97-3"). 7. Rotate clamping lever clockwise (→ "Fig. 97-2"). 7.4.2 Mounting the clamping top onto the segment arc Fig. 98 To ensure a proper sectioning result, the clamping top (→ "Fig. 98-2") must be securely clamped onto the segment arc (→... -
Page 91: Cleaning And Maintenance
• Before each cleaning, switch off the instrument and disconnect the power plug from the mains. Spray proper solvents or cleaners on a cleaning pad and not directly on the instrument to prevent liquids from entering. If liquids go into interior of instrument, contact Leica Biosystems service. Warning The liquids go into the interior of the instrument. - Page 92 Cleaning and Maintenance Caution Use of improper solvents or cleaners, or sharp/hard tools to clean the instrument or accessories. Potential instrument malfunction or delay of diagnosis. • Do not use any solvents containing acetone or xylene for cleaning. • When using cleaners, please comply with the safety instructions of the manufacturer and the laboratory safety regulations.
- Page 93 4. Loosen the eccentric bolt using Allen key No. 4 and remove the segment arc from the blade holder base. 5. Clean all parts of the two-in-one blade holder E. Caution During cleaning, the parts of blade holders are mixed up. Poor section quality. • Do not mix up blade holders during cleaning. HistoCore MULTICUT...
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Page 94: Maintenance
Cleaning and Maintenance 6. Lay the removed parts on an absorbent cloth into the drying chamber (up to a max. of 65 °C) and let the paraffin contamination run off. Warning Removing the parts from the drying chamber (65 °C) during cleaning of the knife/blade holder. Scalding hazard. -
Page 95: Maintenance Instructions
3. Have the instrument inspected on a regular basis by a qualified service technician authorized by Leica Biosystems. The intervals depend on how heavily the instrument is used. We recommend the following inspection intervals depending on the general workload of the instrument... -
Page 96: Lubricating The Instrument
3 seconds in the three-digit display when the TRIM/SECT button is pressed (switching between trimming and sectioning mode). This is a reminder that the instrument must be inspected by an authorized Leica Biosystems service technician, regardless of how heavily the instrument has been used. - Page 97 • Clamping lever (→ "Fig. 105-2") for the blade. Universal cassette clamp Fig. 106 Lubricate the spiral springs (→ "Fig. 106-1") of the tension lever (→ "Fig. 106-2") and move it back and forth several times. HistoCore MULTICUT...
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Page 98: Warranty And Service
Service information If you are in need of technical customer support or spare parts, please contact your Leica Biosystems representative or the Leica Biosystems dealer where you purchased the instrument. Please provide the following information: •... -
Page 99: Decontamination Confirmation
Decontamination Confirmation Decontamination Confirmation Every product that is returned to Leica Biosystems or that requires on-site maintenance must be properly cleaned and decontaminated. You can find the dedicated template of the decontamination confirmation on our website www.LeicaBiosystems.com within the product menu. This template has to be used for gathering all required data. - Page 100 Notes Version 1.2, Revision C...
- Page 102 Leica Biosystems Nussloch GmbH Heidelberger Str. 17 - 19 D-69226 Nussloch Germany Tel.: +49 - (0) 6224 - 143 0 Fax: +49 - (0) 6224 - 143 268 Web: www.LeicaBiosystems.com...