Leica THERMOBRITE ELITE User Manual page 74

6. Using the ThermoBrite Elite Software
Flush
Use this option to add a Flush step. Use a Flush step to ensure previous reagents have been flushed out of the fluidic
lines. This is particularly important when using reagents of high or low pH or solvents. Including a Flush step can increase
the life of the peristaltic tubing.
When needed, the Flush step should be added after a Fill step has been selected. The default flush volume is 35.0mL.
A Flush step of ethanol/reagent grade alcohol/industrial methylated spirits must be programmed
immediately after each fill of xylene substitute (d-Limonene).
A Flush step of distilled/deionized water must be programmed immediately after each fill of any low pH
reagents.
1. Select the Fill step to precede the Flush step, and then click the Flush button.
2. In the Reagent to flush with drop-down list, select a reagent.
3. In the Bottle drop-down list, select a bottle number.
4. Accept the default or clear the default check box and adjust the Flush volume to a value greater than 35mL.
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Figure 6-30: Protocol Reagent Step—Flush
ThermoBrite Elite User Manual
3855-7500-501 B02