Leica RM2245 Instructions For Use Manual
  1. Manuals
  2. Brands
  3. Leica Manuals
  4. Laboratory Equipment
  5. RM2245
  6. Instructions for use manual

Leica RM2245 Instructions For Use Manual

Rotary microtome
Hide thumbs Also See for RM2245:
Table of Contents

Advertisement

Quick Links

See also: Instruction Manual
Instructions for Use
RM2245
Rotary Microtome
Instructions for Use
Leica RM2245
V 1.9 RevC, English - 06/2013
Order No. 14 0501 80101 RevC
Always keep this manual with the instrument.
Read carefully before working with the instrument.

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the RM2245 and is the answer not in the manual?

Questions and answers

Related Manuals for Leica RM2245

Summary of Contents for Leica RM2245

  • Page 1 Instructions for Use RM2245 Rotary Microtome Instructions for Use Leica RM2245 V 1.9 RevC, English - 06/2013 Order No. 14 0501 80101 RevC Always keep this manual with the instrument. Read carefully before working with the instrument.
  • Page 3 Leica reserves the right to change technical spec- thorough investigation in this field. ifications as well as manufacturing processes We are under no obligation to update the present without prior notice.

  • Page 4: Table Of Contents

    TABLE OF CONTENTS Important Information ..........................4 Symbols in the text and their meanings ....................4 Qualification of personnel ........................... 5 Intended use of instrument ......................... 5 Instrument type ............................. 5 Safety ................................. 6 Safety notes ..............................6 Warnings ............................... 6 Integrated safety devices ...........................
  • Page 5 Cleaning and Maintenance ........................66 Cleaning the instrument ..........................66 8.2.1 Replacing fuses ............................68 Maintenance ............................... 68 8.2.2 Maintenance instructions ......................... 69 8.2.3 Lubricating the instrument ........................70 Warranty and Service ........................... 71 Decontamination Certificate ....................... 72 Leica RM2245...

  • Page 6: Important Information

    Important Information Symbols in the text and their meanings Symbol for labeling electrical and elec- tronic equipment in accordance with Section 7 of the German Electrical and Warnings Electronic Equipment Act (ElektroG). appear in a gray box and are marked ElektroG is the law on the bringing into by a warning triangle circulation, return and environmentally...

  • Page 7: Qualification Of Personnel

    It is designed for cutting soft paraffin-embedded specimens as well as harder specimens, as long as they are suitable for being cut manually. The Leica RM2245 is also suitable for IVD (in vitro diagnostics). Any other use of the instrument will be consid-...

  • Page 8: Safety

    The protective devices located on the instrument and the accessories must not be removed or modified. Only service personnel qualified by Leica may repair the instrument and access the instrument's internal components. Warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention.
  • Page 9 • The protective devices located on the instrument and the accessories must not be removed or modified. Personal safety precautions • When working with microtomes, personal safety precautions must always be taken. It is mandatory to wear work safety shoes, safety gloves, a mask and safety goggles. Leica RM2245...
  • Page 10 Safety Safety instructions - working with the instrument • Take care when handling microtome knives and disposable blades. The cutting edge is extremely sharp and can cause serious injuries! • Always remove the knife / blade before detaching the knife holder from the instrument. Always put the knives back into the knife case when not in use! • Never place a knife anywhere with the cutting edge facing upwards and never try to catch a falling knife!

  • Page 11: Integrated Safety Devices

    Fig. 2 When using both brake systems at the same time, always move the lever (3) to position first. Otherwise, it may be  not be possible to release the lever (5). Fig. 3 Leica RM2245...
  • Page 12 Safety Knife guard on the knife holder Knife holder N Each knife holder is equipped with a tightly mounted knife guard (8, 9). This makes it possible to cover completely the cutting edge in every knife or blade position. Knife holder N/NZ The knife guard (8) of the knife holder N/NZ can be easily positioned via the two handles (7) (Fig.

  • Page 13: Instrument Components And Specifications

    Smooth-turning Handwheel Lever of Handwheel brake Knife guard on the blade holder Separate Control panel Section waste tray Clamping lever for lateral displacement Blade holder E with lateral displacement Knife/blade Fig. 6 holder base Leica RM2245...

  • Page 14: Instrument Specifications

    Main switch Power supply Fig. 7 Instrument specifications The Leica RM2245 is a semi-motorized rotary The retraction value can be selected as required. microtome with a light-action handwheel. The motorized specimen head can be moved at The RM2245 supports two manual sectioning two speeds (300 µm/s and 900 µm/s).

  • Page 15: Technical Data

    Working height (knife blade): 168 mm (measured from the table) Weight (without accessories): approx. 37 kg Control panel Width: 94 mm Depth: 164 mm Height: 50 mm Height (in inclined position): 81 mm Weight (net): approx. 0.450 kg Leica RM2245...
  • Page 16 Instrument components and specifications Microtome Sectioning thickness setting: Section thickness setting range: 0.50 - 100 µm Setting values: from 0.50 - 5.0 µm in 0.5 µm increments from 5.0 - 20.0 µm in 1.0 µm increments from 20.0 - 60.0 µm in 5.0 µm increments from 60.0 - 100.0 µm in...

  • Page 17: Instrument Setup

    Instrument Setup Standard delivery The Leica RM2245 standard delivery includes: 1 Leica RM2245 basic instrument, without orientation ..........14 0501 38178 1 handwheel, complete ....................14 0501 38181 1 external control panel ....................14 0501 38179 1 section waste tray ......................14 0502 37931 1 set of power cables consisting of: 1 power cable for Germany ..................

  • Page 18: Unpacking And Installation

    Instrument Setup Unpacking and installation When the instrument is delivered, check the tilt indicators on the packaging. If the arrowhead is blue, the shipment was transported laying flat, was tilted at too great an angle or fell over during transport. Note this on the shipping documents and check the shipment for possible damage.
  • Page 19 • To move the instrument, hold it by the front of the base plate, lift it up gently and slide it on its slides. Observe the correct resting angle to the table to avoid pinching your fingers. Fig. 10 Leica RM2245...

  • Page 20: Assembling The Handwheel

    (outlets) of the country of use. Do not use an extension cable! Checking the voltage Before connecting the instrument to The Leica RM2245 can be connected to various the power supply, be absolutely cer- electrical power grids (depending on voltage and tain to check that the voltage selec-...
  • Page 21 The connecting cable (15) of the control panel is fixed to the microtome. It may not be discon- nected. • Insert the plug (14) of the connecting cable (15) into the connecting jack (16) on the rear of the control panel. • To fasten the plug, tighten the two screws (17). Fig. 13 Leica RM2245...

  • Page 22: Switching On The Instrument

    Instrument Setup Switching on the instrument Exposure to extreme temperature changes and high air humidity may cause condensation to form inside the instrument. After transporting, please wait at least 2 hours to allow the instrument to adopt the ambient temperature before turning it on! Failure to comply with this may cause damage to the instrument.

  • Page 23: Operation

    CLEAR button counter. Resets section counter and section thickness sum (to 0). MENU MODE + CLEAR Pressing them simul- taneously switches to the setting of the Fig. 14 retraction value. Leica RM2245...

  • Page 24: Control Panel

    Operation 5.1.2 Control panel Three-digit display for section thick- ness/trimming sec- tion thickness LED TRIM (green) Lights up when trim- ming mode is active. LED SECT (green) Buttons Lights up when for setting the section sectioning mode is thickness/trimming active. section thickness Button to enable and dis-...

  • Page 25: Display And Control Elements

    With the double-arrow buttons, the coarse feed operates at 900 µm/s; with the single-arrow buttons, it runs at 300 µm/s. In sectioning mode the coarse drive can be used in STEP mode, or with continuous feed. The instrument is delivered with continuous feed (standard configuration). Leica RM2245...
  • Page 26 (ensure that version number 2.1 was displayed). This feature is only available in sectioning mode in version 2.1 or Multiple Single higher. For software versions older than 2.1, contact Leica Technical Service. step step forward forward • Press the TRIM/SECT...
  • Page 27 The retraction value can be selected in 5 µm increments between 5 and 100 µm. Specimen retraction is set to 10 µm at the factory. Specimen retraction can be disabled if required. The selected setting is maintained when the instrument is turned off. Leica RM2245...
  • Page 28 Operation Configuring the retraction settings • To call up the retraction settings, press the MENU MODE and CLEAR buttons simultaneously. • The current set value is displayed as a three-digit number in the four-digit display (e.g. "025" = 25 µm). • Select the desired retraction value. The retraction value can be adjusted in increments of 5 µm to a maximum of 100 µm using the buttons on the control panel.
  • Page 29 • You can continue to work on the specimen by moving it back a short distance using the coarse feed buttons (set trimming mode!). • The STEP function is disabled in the remaining feed range. Leica RM2245...
  • Page 30 Operation Directional specimen holder fixture In the quick clamping device of the directional specimen holder fixture, all specimen clamps available as optional accessories can be used (implemented). The specimen orientation allows for simple posi- The directional specimen holder fixture may be tion correction of the specimen surface when the exchanged for a non-directional fixture (optional specimen is clamped into place.
  • Page 31 Use the Allen key provided, size 5 (with handle!) for the adjustment. • If the specimen head moves downwards, turn the screw approx. turn clockwise. • If the specimen head moves upwards, turn the screw (34) approx. turn counterclockwise. • Continue this procedure until the specimen head no longer moves once released. Fig. 21 Leica RM2245...

  • Page 32: Inserting The Knife Holder

    Operation Inserting the knife holder Setting up the knife holder base • Release the clamping lever (50) by rotating it counterclockwise. • Insert the knife holder base (51) using the notch (52) on the bottom into the T-piece (55) of the microtome base plate (53). • To secure the knife holder base, turn the clamping lever (50) clockwise.

  • Page 33: Inserting The Universal Cassette Clamp

    (61) clockwise as far as it will go. Fig. 24 Since all stage clamps available as accessories are equipped with the same kind of guide on the back, they are inserted in the same way described here using the example of the cassette clamp. Leica RM2245...

  • Page 34: Adjusting The Clearance Angle

    Operation Adjusting the clearance angle • The index marks (0°, 5° and 10°) for adjustment of the clearance angle (59.1) are located on the right side of the knife holder (57). • There is also an index mark (59.2) on the right side of the knife holder base (51) which serves as a reference point when adjusting the clear- ance angle.

  • Page 35: Clamping The Specimen

    "Optional accessories". Clamping the knife / disposable blade Take care when handling microtome knives and disposable blades. The cutting edge is extremely sharp and can cause serious injuries! • Fold knife guard (9) downward. • To insert the blade, flip the right clamping lever (10) forward and down. Fig. 26 Leica RM2245...
  • Page 36 Operation • Carefully push in the blade from above or from the side. Make sure that the blade is clamped in the center and, most importantly, parallel to the upper edge of the pressure plate. • To clamp the blade, rotate clamping lever (10) clockwise back upwards. • To remove the blade, fold the clamping lever (10) downwards counterclockwise. The ejector (9a) ensures safe blade changing. Use the blade ejector to eject the blade! • Another option for removing the blade is to use...

  • Page 37: Cutting Into The Specimen (Trimming)

    When using the knife holder E with lateral displacement, it is sufficient to move the knife holder sideways. • Use the TRIM/SECT key to select the sectioning mode. • Adjust the appropriate sectioning thickness or verify the selected value. • Select conventional sectioning or ROCK mode. • To section the specimen, turn the handwheel clockwise smoothly or rock it back and forth if in ROCK mode. • Pick up the sections and mount them on microscope slides. Leica RM2245...

  • Page 38: Changing The Specimen Or Interrupting Sectioning

    Operation Changing the specimen or interrupting sectioning Lock the handwheel and cover the knife edge with the knife guard prior to any manipulation of knife or specimen head, as well as prior to changing the specimen block and during all work breaks! • Raise the specimen to the upper end position and activate the mechanical handwheel lock.

  • Page 39: Optional Accessories

    • Insert the spring (5a) and pin (5b) with the flatter Fig. 29 side into the thrust piece (5). Completely screw • Finally, place the dovetail holder (2) and fasten in the thrust piece with a flat-tip screwdriver. by screwing in the 4 screws (1) using an Allen • Completely screw in the setscrews (3+4). key size 3. Leica RM2245...

  • Page 40: Fine-Directional Fixture For Specimen Clamps

    Optional Accessories 6.1.3 Fine-directional fixture for specimen clamps • Before the fine-directional fixture for speci- men clamps can be mounted, loosen 4 screws (10) (Allen key No. 3) and carefully remove the fixture for specimen clamps from the base- plate (9). • Using the 4 supplied screws (11) and the Allen key No.

  • Page 41: Quick Clamping System

    It is used as a specimen holder for use with the fine-directional specimen holder fixture with zero point indicators or the directional specimen holder fixture. • Screw the 4 screws (13) into bore A with an Allen key No. 2.5 and tighten them. Fig. 31 Leica RM2245...

  • Page 42: Specimen Clamps And Holders

    Optional Accessories Specimen clamps and holders All specimen clamps available as accessories can be integrated into either the directional or non-directional specimen holder fixture. 6.2.1 Standard specimen clamp The standard specimen clamp is available in two sizes: 40 x 40 mm and 50 x 55 mm. They are designed for direct clamping of rectan- gular blocks.

  • Page 43: Vee Insert

    • Insert the pin (70.1) of the vee insert (70) in the hole (69) of the lower jaw (68). • Mount the specimen as required. • Turn the knurled screw (66) clockwise to move the movable jaw with the vee insert upward 70.1 against the fixed jaw to securely clamp the sample. Fig. 33 Leica RM2245...

  • Page 44: Foil Clamp Type 1

    Optional Accessories 6.2.3 Foil clamp type 1 Clamping of foil pieces • Move the movable jaw (74) to the right as re- The foil clamp type 1 is appropriate both for quired by turning the setscrew with an Allen clamping very small and thin foil pieces and flat, key No.

  • Page 45: Universal Cassette Clamp

    Order No. 14 0502 37999 Fig. 36 Leica / Surgipath cassettes with minimum dimensions of 39.8 x 28 mm and maximum dimensions of 40.9 x 28.8 mm may be clamped in the universal cassette clamp (UCC) horizontally as well as vertically.

  • Page 46: Super Mega-Cassette Clamp

    Optional Accessories 6.2.5 Super mega-cassette clamp Fig. 37 Assembly of the super mega-cassette clamp The super mega cassette clamp should preferably be used with the rigid fixture for specimen clamps. To do so, proceed as follows: Remove the rubber ring (5) only after having attached the cassette clamp on the specimen head! • Screw the rigid fixture for specimen clamps (4) onto the specimen head (3):...

  • Page 47: Holder For Round Specimens

    (92) into the bore (93) and rotate it counter- clockwise to release the clamp. You can now rotate the specimen so that the side you want faces upwards. • To lock it in the position you have chosen, tighten the pin (92) by turning it clockwise. Fig. 38 Leica RM2245...

  • Page 48: Knife Holder Base And Knife Holder

    Optional Accessories Knife holder base and knife holder The plastic handles of all clamping levers on the instrument and knife holders can be turned to the position that is most convenient for each user. Pull the grip (94) out of the lever, hold it in this position, and rotate it to the desired position.

  • Page 49: Knife Holder E/E-Tc

    6.3.2 Knife holder E/E-TC Prior to inserting the blade, both knife The knife holder E-TC is designed holder and knife holder base must for the Leica TC-65 tungsten carbide have been installed on the instrument! blades. Knife holder E-TC Inserting the blade, knife holder E and E-TC • Fold knife guard (9) downward.
  • Page 50 Leica Biosystems offers special prices for new clamp mounts in case of damages to the clamp mount after the warranty has expired. In this way, perfect function of the device can be ensured over the course of many years.
  • Page 51 The vessel is filled with water up to the blade. After trimming, remove the section waste from the tray and create the sections to be prepared. The sections floating on the surface of the water can be removed using the glass slide. Fig. 45 Leica RM2245...

  • Page 52: Knife Holder N/Nz

    Optional Accessories 6.3.3 Knife holder N/NZ The knife holders N and NZ are appropriate for standard steel and tungsten carbide knives, profile c and d, up to 16 cm long. The integrated height adjustment feature al- lows you to also use knives that have been resharpened numerous times. Mounting the knife support bar • Push knife guard (8) to the center.
  • Page 53 • Loosen the clamping screws (49) by turning them counterclockwise. • Push the knife (47) to the left or right as required. • To clamp the knife (47), always tighten the clamping screw (49) first which is located on the side to which the knife has been repositioned by turning it clockwise. Leica RM2245...

  • Page 54: Blades/Knives

    Optional Accessories Blades/knives 6.4.1 Disposable blades Disposable blades - low profile (819) 80 x 8 x 0.25 mm 01 package of 50 pcs......14 0358 38925 10 package of 50 pcs......14 0358 38382 Fig. 47 Disposable blades - high profile (818) 80 x 14 x 0.35 mm 01 package of 50 pcs.
  • Page 55 Order No..........14 0216 04206 Fig. 51 Knife case Variable knife case (plastic), for 1 or 2 knives: 10 - 16 cm long (Carbide metal or SM2500 knife: only for 1 knife!) Order No..........14 0213 11140 Fig. 52 Leica RM2245...

  • Page 56: Section Waste Tray

    Optional Accessories Section waste tray • Push the section waste tray (18) from the front to the microtome base plate (53) until it is held in place by the two magnets (39) (on the front of the microtome base plate). To remove the section waste tray, lift it slightly and pull it off towards the back.

  • Page 57: Tray

    (Fig. 44) must be removed. • Remove the freezer plate from the insulation jacket and place it in a deep freezer, ideally overnight (but for at least six hours) at approx. -23 °C. Fig. 56 • Reassemble the freezer plate and insulation jacket and place them on the bench or the instrument. Leica RM2245...

  • Page 58: Universal Microscope Carrier

    (c) Fig. 57 3 - Horizontal arm with cross-member (d) and support ring (e) 4 - Support plate, large (for RM2235, RM2245 and RM2255) 5 - Support plate, small (for RM2265) 6 - Allen key, size 3...
  • Page 59 • Slide the horizontal arm (3), flat side facing the lock screw (g), into the cross-member (d) and tighten. For more information on connecting and using the microscope, magnifying lens or cold-light source, please see the appropriate Instructions for Use. Fig. 62 Leica RM2245...

  • Page 60: Magnifying Lens

    Optional Accessories 6.10 Magnifying lens The magnifying lens provides a 2x mag- nification and can be used with all Leica 2200 series rotary microtomes. • Open the screw (3) on the horizontal arm of the microscope carrier in a counterclockwise direction. • Insert the silver connection piece (1) as far as it will go.

  • Page 61: Cold Light Source

    • Switch on the Cold light source using the power switch (7). • Remove the protective caps (8) and align the light guide with the specimen. Fig. 67 Fig. 69 Leica rotary microtome with installed optional Fig. 68 accessories: Magnifier and fiber-optic light guide Leica RM2245...

  • Page 62: Ordering Information

    Optional Accessories 6.13 Ordering information Fixture for specimen clamps, rigid, silver ..............14 0502 38160 Fixture for specimen clamps, directional, silver ............14 0502 38949 Fixture for specimen clamps, directional, with 2 zero point indicators, silver ..14 0502 37717 Quick clamping system assembly ...................
  • Page 63 Magnifying lens assembly ....................14 0502 42790 Cold light sources Leica CLS 100X, 100-120 V/50-60 Hz ................14 0502 30214 Leica CLS 100X, 230 V/50-60 Hz .................. 14 0502 30215 Leica CLS 100X, 240 V/50-60 Hz .................. 14 0502 30216 Fiber-optic light guide ......................

  • Page 64: Troubleshooting

    (service life approx. 7 years). • Proceed as described above. • Work normally after checking the settings. • Have the battery replaced by Leica service as soon as possible. LEDs (40), (41) in the coarse feed buttons are lit at the same time. This indicates a defect in the detection of the STOP and HOME positions of the specimen head.

  • Page 65: Malfunctions, Possible Causes And Troubleshooting

    The following is a list of the most common problems which can arise while working with the instrument, along with possible causes and troubleshooting procedures. If the malfunction cannot be remedied with any of the options listed in the table, or the problem occurs repeatedly, inform Leica Service Support immediately. Problem Possible cause Corrective action 1.

  • Page 66: Possible Faults

    • When using cassettes, par- ticularly thin-walled cassettes, from a company other than Leica or Surgipath, the cassette may become deformed or other clamping problems may arise. If while trying to clamp the cas- sette you realize that it is not...
  • Page 67 If necessary, tighten the levers and screws. • Too great of a sectioning force • Adjust the sectioning speed and/ 5. High blade consumption was applied. or section thickness when sec- tioning. Select a thinner section. Leica RM2245...

  • Page 68: Cleaning And Maintenance

    Cleaning and Maintenance Cleaning the instrument Always remove the knife / blade before detaching the knife holder from the instrument. Always put the knives back into the knife case when not in use! Never place a knife anywhere with the cutting edge facing upwards and never try to catch a falling knife! When using cleaning agents, observe the manufacturer's safety instructions and the laboratory regulations valid in the country of use.
  • Page 69 (see also Chap. 8.2.3). • When installing the pressure plate (83) ensure that the upper edge is parallel to and level with the rear edge of the knife holder base (86) (refer also to Chap. 6.3.2, Fig. 43). Fig. 71 Leica RM2245...

  • Page 70: Replacing Fuses

    Cleaning and Maintenance Universal cassette clamp • Detach cassette clamp (13) for a thorough cleaning, removing all paraffin residues. • For cleaning, do not use xylene. Use xylene substitutes or paraffin remov- ers such as "Para Gard." • The cassette clamp (13) can also be placed in an oven heated to a maxi- mum of 65 °C, until the liquid wax escapes. • Remove paraffin residues with a dry cloth.

  • Page 71: Maintenance Instructions

    ò when the TRIM/SECT button is pressed (switching between trimming and sectioning mode). This is a reminder that the instrument must be inspected by an authorized Leica service technician, regardless of how heavily the instrument has been used. Leica RM2245...

  • Page 72: Lubricating The Instrument

    Cleaning and Maintenance 8.2.3 Lubricating the instrument Once a month, lubricate the following parts with the included drive parts oil No. 405 (1 - 2 drops are enough). Instrument and specimen holder: • The clamping key (95) of the quick clamping system. • The T-piece (55) on the microtome base plate. • Guide rails (96) for the knife holder base on the microtome base plate.

  • Page 73: Warranty And Service

    Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control proce- dure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted.

  • Page 74: Decontamination Certificate

    Dear Customer, Any product that is to be returned to Leica Biosystems or serviced on site, must be cleaned and decontaminated in the appropriate manner. Since it is not possible to decontaminate for prion diseases, such as CJD, BSE, CWD etc., equipment exposed to specimens containing prion diseases cannot be returned to Leica Biosystems for repair.
  • Page 75 Such equipment must not be returned without the written agreement of Leica Biosystems. The equipment has been prepared to ensure safe handling/transportation. Decontamination Certificate Whenever possible, please use the original transportation case/box. Important - to avoid refusal of shipment: Place one copy in the unit prior to packaging, or hand it over to the service engineer. Customer assumes all responsibility for the immediate return shipment of articles sent to Leica without proper decontamination documentation.
  • Page 76 Notes Instructions for Use V 1.9 RevC - 06/2013...
  • Page 78 Leica Biosystems Nussloch GmbH Heidelberger Straße 17-19 D- 69226 Nussloch Phone: +49 - (0) 6224 - 143 0 Fax: +49 - (0) 6224 - 143 268 Web: www.LeicaBiosystems.com...

Table of Contents

Save PDF