Leica THERMOBRITE ELITE User Manual page 72

6. Using the ThermoBrite Elite Software
Reagent Temp
Use this option to define the temperature at which each chamber must be for the reagent step. The default is 37°C.
1. Double-click Temp. Enter a temperature between 25°C and 95°C.
2. Do not uncheck On. This will disable heating and cooling for the module.
Selecting the On check box only enables heating/cooling for the module. Only modules that are
loaded with slides at the time of operation will actually heat or cool.
3. Select when you want the instrument to start heating/cooling:
Select the Wait for temp before continuing check box to prevent the next step from starting until the
l
temperature has achieved the selected temperature.
Clear the Wait for temp before continuing check box to start the incubation time while the instrument
l
begins to heat/cool toward the target temperature. At the end of the incubation time, it will then proceed
to the next step. In general, when cooling, clear the check box.
4. Click Update to validate the entry. The Reagent Step screen is displayed. After updating, a Wait: Y or Wait: N will
appear on the Reagent Step screen to the right of the temperature, indicating whether the "Wait for temp before
continuing" check box was checked.
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Figure 6-28: Protocol Reagent Step—Temperature
ThermoBrite Elite User Manual
3855-7500-501 B02