Preparing The System For Run; Reagents; Compatible Reagents - Leica THERMOBRITE ELITE User Manual

5.

Preparing the System for Run

5.1

Reagents

5.1.1

Compatible Reagents

The following reagents may be used in the ThermoBrite Elite:
Saline Sodium Citrate (SSC)
l
Phosphate Buffered Saline (PBS)
l
2-(N-morpholino) Ethanesulfonic Acid (MES)
l
Tris-HCL
l
Citrate Based Buffers
l
Proteases
l
Sodium Thiocyanate
l
0.2N Hydrochloric Acid (and further dilutions)
l
Ethanol
l
Isopropanol
l
Methanol
l
4% Formaldehyde
l
10% Neutral Buffered Formalin
l
Hydrogen Peroxide
l
Deionized/Distilled Water
l
d-Limonene/Clearene
l
This list may not be all-inclusive.
Contact Technical Support before using reagents not included above.
Reagents other than those listed may damage some components of the instrument. Do NOT use xylene in
the instrument.
A Flush step of ethanol/reagent grade alcohol/industrial methylated spirits is required after each fill of
xylene substitute (d-Limonene).
A Flush step of distilled/deionized water is required after each fill of any low pH reagents.
ThermoBrite Elite User Manual
3855-7500-501 B02
45