Run A Blanking Cycle - Thermo Scientific NanoDrop Lite Plus User Manual

1 NanoDrop Lite Plus User Guide
Helpful Information for Measuring Samples

Run a Blanking Cycle

20 NanoDrop Lite Plus User Guide
Run a blanking cycle to verify the following:
• instrument is operating normally (with flat baseline)
• pedestals are clean (i.e., no dried-down sample material on pedestals)
• absorbance contribution of buffer solution you plan to use for sample analyses
Supplies needed
• lint-free laboratory wipes
• calibrated precision pipettor (0–2 μL)
• buffer solution for evaluation
To run a blanking cycle
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
1. From the Home screen, select an application.
2. Lift the instrument arm and clean the upper and lower pedestals with a
laboratory wipe.
3. Measure a water blank:
– Pipette exactly 1 μL deionized water (DI H
lower the arm.
– Tap Blank and wait for the measurement to complete.
– Lift the arm and clean both pedestals with new laboratory wipe.
4. Measure the buffer solution:
– Pipette 1-2 μL buffer solution onto the pedestal, lower the arm, and tap
Measure
– Wait for measurement to complete.
The resulting absorbance should vary no more than 0.04A at the analysis
wavelength.
If your spectrum does not meet these criteria, repeat steps 2–4.
If spectrum is still outside specifications, see
on page 21
O) onto the lower pedestal and
2
"Solutions for blanking problems"
Thermo Scientific