Thermal - Thermo Scientific Vitrobot Mk IV User Manual

Chapter | Safety
DANGER! Indications that there are lethal voltages present in the electronics compartment.
Take necessary precautions when removing the covers.
Note Users are not allowed to take of any cover. Lethal voltages are present inside the
Vitrobot.
•
Users should notify Thermo Fisher Scientific service immediately when any electrical error
occurs.
Fuses
Only trained service personnel should replace fuses. Replace only with fuses of the same
type, voltage rating, and current.

2.5 Thermal

CAUTION! Cold substances could be present. Extremely low temperatures are reached using
Liquid Nitrogen. Precautions must be taken where this sticker is visible.
Metallic parts of Vitrobot liquid nitrogen container
The Vitrobot will be provided with metallic parts, which are cooled with liquid nitrogen during
use. During normal use, these get extremely cold (-196 °C). These metallic accessories, once
cold, should not be handled by hand directly, but with appropriate tweezers covered with an
insulating material, to avoid skin burn.
Liquid Ethane/Propane
When used in the appropriate small container provided with the Vitrobot accessories, a small
volume (~4 ml) of liquid ethane/propane is formed by cooling the gases at liquid nitrogen
temperature; there is a risk of skin burns upon direct contact with liquid ethane or liquid
propane or a mixture of both. Use protective eyewear during the entire session.
•
Ethane and Propane gases are highly flammable and, along with Nitrogen, pose an
asphyxiation hazard. They should only be used in a well-ventilated, spark-free
environment. It is recommended to use the Vitrobot in a fume hood, installed in a lab
where there is an Oxygen level detector.
•
It is recommended that a small Ethane/Propane gas bottle is used in the lab where the
Vitrobot is installed; usually 2.5 l – 5.0 l; the reduced dimensions should facilitate storage
of the gas bottle in a fire-proof cabinet when not in use.
•
A pressure regulator with two dedicated gauges (100 psi to 5 psi) is needed to extract the
gas in a regulated, constant and safe manner.
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PN 103261 | Revision D | 23-Oct-2019
User Manual
Page 12