PrimeLab 1.0 MULTITEST User Manual page 171

Legionella
(147)
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46) Swirl complete unit (without lids!) for 10 seconds until particles are
suspended.
47) Push magnet towards/against cuvettes LG-MHCB1 and LG-MHCB2.
48) Press OK to start 03:00 minutes countdown.
49) Empty LG-MHCB1 and LG-MHCB2 (still in magnetic holder LG-MH) to
the BACK whilst keeping magnet in place by pushing towards cuvettes
with your thumb.
50) Brake seal of "L4 Enzyme 2", remove plastic protector, push plunger
all the way down and shake vigorously for 10 seconds.
51) Pull magnet towards you and away from cuvettes LG-MHCB1 and LG-
MHCB2.
52) Add exactly 1ml of "L4 Enzyme 2" to each cuvette LG-MHCB1 and
LG-MHCB2. Clean pipette needed in case you were not supplied with
single doses.
53) Swirl complete unit (without lids!) for 10 seconds until particles are
suspended.
54) Shake both cuvettes (LG-MHCB1 and LG-MHCB2), still placed in the
magnetic holder and with magnet pulled away from the cuvettes, gently for
2 minutes.
55) Press OK to start 02:00 minutes countdown.
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56) Add 3 drops or 100 µl "L5 Stopping Reagent" to both cuvettes LG-
MHCB1 and LG-MHCB2 and swirl for 5 seconds WITHOUT lids.
57) Push magnet towards/against cuvettes LG-MHCB1 and LG-MHCB2.
58) Press OK to start 05:00 minutes countdown.
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59) Transfer 1ml from LG-MHCB1 (left) to a new, clean small vial "LG-CB".
USE A CLEAN PIPETTE.
60) Place the black plastic "Legionella adapter" (PLSp-LegiAD-1) properly
into the PrimeLab measuring chamber. Make sure it fits properly.
61) Place filled 1ml LG-CB vial into the adapter. Place the light shield
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(PLSp-LS-1) on top and press ZERO.
62) Remove light shield and vial (LG-CB) but NOT the adapter from the
PrimeLab.
138
60 - 1000000 cfu/test
Name on device: 147-Legionella-liq
Liquid
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138