PrimeLab 1.0 MULTITEST User Manual page 169

Legionella
(147)
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13) Shake both cuvettes (LG-MHCB1 and LG-MHCB2), still placed in the
magnetic holder and with magnet pulled away from the cuvettes, gently by
inverting 3 times every 3 minutes for 15 minutes.
14) Press OK to start 15:00 minutes countdown.
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15) Remove and discard lids from LG-MHCB1 and LG-MHCB2.
Lids shall NOT be used for further steps!
16) Push magnet towards/against cuvettes LG-MHCB1 and LG-MHCB2.
17) Press OK to start 05:00 minutes countdown.
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18) Empty LG-MHCB1 and LG-MHCB2 (still in magnetic holder LG-MH) to
the BACK whilst keeping magnet in place by pushing towards cuvettes
with your thumb.
19) Pull magnet towards you and away from cuvettes LG-MHCB1 and LG-
MHCB2.
20) Add "L2 washing buffer" to both cuvettes LG-MHCB1 and LG-MHCB2
up to line 2 (4.5ml).
21) Swirl complete unit (without lids!) for 10 seconds until particles are
suspended.
22) Push magnet against cuvettes LG-MHCB1 and LG-MHCB2.
23) Press OK to start 03:00 minutes countdown.
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24) Empty LG-MHCB1 and LG-MHCB2 (still in magnetic holder LG-MH) to
the BACK whilst keeping magnet in place by pushing towards cuvettes
with your thumb.
25) Pull magnet towards you and away from cuvettes LG-MHCB1 and LG-
MHCB2.
26) Add exactly 1ml of "L3 Enzyme 1" to each cuvette LG-MHCB1 and
LG-MHCB2. Clean pipette needed in case you were not supplied with
single doses.
27) Shake both cuvettes (LG-MHCB1 and LG-MHCB2), still placed in the
magnetic holder and with magnet pulled away from the cuvettes, gently
every 2 minutes for 10 minutes.
28) Press OK to start 10:00 minutes countdown.
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136
60 - 1000000 cfu/test
Name on device: 147-Legionella-liq
Liquid
Continued...
136