To Condition A Capillary Column - Agilent Technologies 5977B Series Operating Manual

To Condition a Capillary Column

Materials needed
• Carrier gas, (99.9995% pure or better)
• Wrench, open-end, 1/4-inch and 5/16-inch (8710-0510)
Do not condition your capillary column with hydrogen. Hydrogen accumulation in
WA R N I N G
the GC oven can result in an explosion. If you plan to use hydrogen as your carrier
gas, first condition the column with ultrapure (99.999% or better) inert gas such as
helium, nitrogen, or argon.
The GC operates under high temperatures. Do not touch any GC parts unless you are
WA R N I N G
certain they are cool.
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Procedure
1 Install the column in the GC inlet. (See
2 Set a minimum velocity of 30 cm/s, or as recommended by the column
3 Program the oven from room temperature to the maximum temperature
4 Increase the temperature at a rate of 10 to 15 °C/min.
5 Hold at the maximum temperature for 30 minutes.
Never exceed the maximum column temperature, either in the GC/MS interface, the
C A U T I O N
GC oven, or the inlet.
6 Set the GC oven temperature to 30 °C, and wait for the GC to become ready.
7 Attach the column to the GC interface. (See
5977B Series MSD Operating Manual
Split/Splitless Inlet"
manufacturer. Allow the carrier gas to flow through the column at room
temperature for 15 to 30 minutes to remove air.
limit for the column.
in the GC/MS Interface Using the Self-Tightening Column Nut"
"To Install a Capillary Column in a
on page 38.)
Installing 7890 GC Columns
"To Install a Capillary Column
on page 42.)
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