3D Histech Pannoramic 250 FLASH II User Manual page 96

Pannoramic 250 FLASH II 1.18.1 User's Guide
Focus
To define focus limits, select Limiting the range checkbox. Set the upper and lower limits by
selecting the desired value in the selection boxes. This is useful if Auto Focus does not find
the focus on the specimen, but on the coverslip. Also, this makes the focusing sequence
faster.
By using Set Current, the Lower limit value is set to -32 of the current focus value and the
Upper limit value is set to +32 of the current focus value.
Use the two arrow buttons to focus manually. This is useful when you are interested in an
area that remained blurry with Auto Focus (for example, the specimen is thicker in that
area). This does not influence the way slides are digitized in Automatic mode. The manual
focus is mainly for setup purposes, not for using the product as a traditional microscope.
Define the manual focus step size by adjusting the Step size slider. Search for the sharpest
focus on the current field of view automatically by clicking
Manual Focus
By selecting the Use manual focus box, you can preset the coarse focus value. By activating
the Coarse focus function, the lower limit value is set to -64, and the upper limit value is set
to +64 of the current focus value.
Before entering coarse focus value, turn live image on and set a
suitable focus position.
You can accept the current z-value by clicking Set Value.
If you select the Interpolated focus option, the coarse focus level is calculated from three
different focus points of the specimen. Carry out a manual focusing sequence in the
preview image on those three points.
Set the correct focus value of the desired preview position by clicking Set. If you want to go
back to any of the previously set focus points, click the appropriate Goto button. To delete
the stored values, click Clear Values. The software calculates a coarse focus level from the
three values determined this way as a basis for the fine focusing routine. You can only
define the start parameter here. This will, however, speed up the focusing process.
If you deselect the Use fine focus checkbox, the software immediately starts scanning on
the basis of the interpolated values, without pre-fine-focusing.
May 29, 2014 – Rev. 1
Note:
3DHISTECH Ltd.
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