3D Histech TMA Grand Master 2.0 User Manual
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3D Histech TMA Grand Master 2.0 User Manual

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TMA Grand Master
2.0
User's Guide
November 5, 2013
GM2.0-UG-EN Rev1
Rev. 1

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Summary of Contents for 3D Histech TMA Grand Master 2.0

  • Page 1 TMA Grand Master User’s Guide November 5, 2013 GM2.0-UG-EN Rev1 Rev. 1...

  • Page 2: Table Of Contents

    TMA Grand Master 2.0 – User’s Guide Contents Disclaimer............................4 Declaration Of Conformity......................6 Character Formats and Symbols....................7 Terms and abbreviations......................8 1 Intended Use..........................9 1.1 Main Features of TMA Grand Master....................9 2 TMA Grand Master Hardware....................10 2.1 TMA Grand Master Main Unit......................10 2.2 Cabling..............................11...
  • Page 3 TMA Grand Master 2.0 – User’s Guide 5.5 Launching the transfer process......................42 5.6 Slide overlay............................48 5.7 Exporting donor block data........................54 6 Maintenance of TMA Grand Master..................60 6.1 Preventive Maintenance of the Main Unit...................60 6.2 Cleaning Tasks............................60 6.2.1 Opening the Service Compartment Door.......................61 6.3 Replacing fuses of the main power switch...................62...

  • Page 4: Disclaimer

    TMA Grand Master 2.0 – User’s Guide Disclaimer Disclaimer Copyright © 2001-2013 3DHISTECH Ltd. All rights reserved. Intellectual property related to the 3DHISTECH products are as follows: EP 1,789,831; US 7,663,078; EP 2,024,772; JP 2009-512685; US 12/301,781; EP 1,644,767; JP 2007- 516451;...
  • Page 5 TMA Grand Master 2.0 – User’s Guide Disclaimer CAUTION For research and education uses only, not for use in diagnostic procedures. This product has not been approved or cleared as a medical device by the U.S. Food and Drug Administration, nor any other regulatory authority.

  • Page 6: Declaration Of Conformity

    TMA Grand Master 2.0 – User’s Guide Declaration Of Conformity Declaration Of Conformity 3DHISTECH Ltd. declares that the product TMA Grand Master is designed and produced with consideration of specified requirements according to the IEC 61326-2-6:2005 standard, and conforms to the directives and other standards listed below: IEC 61010-1:2001 (ed.

  • Page 7: Character Formats And Symbols

    TMA Grand Master 2.0 – User’s Guide Character Formats and Symbols Character Formats and Symbols Example Abbreviation or term that is explained in the section Terms and Abbreviations. Example Words or characters that appear on the screen. These include field names, screen titles, pushbuttons and menu names, paths or options.

  • Page 8: Terms And Abbreviations

    TMA Grand Master 2.0 – User’s Guide Terms and abbreviations Terms and abbreviations Digital slide / Slide Slide digitized with a scanner microscope, for example Pannoramic SCAN. Consists of an MRXS file and a folder with the same name as the MRXS file.

  • Page 9: Intended Use

    TMA Grand Master 2.0 – User’s Guide 1 Intended Use 1 Intended Use TMA Grand Master is a software-controlled electronic laboratory device that automates the creation of tissue microarrays (TMAs) and also the process of PCR sampling. The creation of TMA blocks (by taking tissue core samples from donor blocks and inserting them into recipient blocks) and the insertion of extracted tissue cores into PCR tubes is controlled by using a graphical user interface.

  • Page 10: Tma Grand Master Hardware

    TMA Grand Master 2.0 – User’s Guide 2 TMA Grand Master Hardware 2 TMA Grand Master Hardware Hardware: TMA Grand Master main unit • Control computer with a monitor, a mouse and a keyboard • USB cables between the main unit and the control computer •...

  • Page 11: Cabling

    TMA Grand Master 2.0 – User’s Guide 2 TMA Grand Master Hardware 6. Grips for service compartment door 7. Front power supply switch Back View Figure 2 – TMA Grand Master back view 1. Terminal point for the power supply cord 2.

  • Page 12: Delivery

    TMA Grand Master 2.0 – User’s Guide 2 TMA Grand Master Hardware Important! The use of an uninterruptible power supply (UPS) is recommended but optional. Warning! Only connect external devices that are safety extra low voltage rated to the TMA Grand Master instrument to avoid the risk of electrical shock.

  • Page 13: Installing And Configuring Tma Grand Master Hardware

    TMA Grand Master 2.0 – User’s Guide 2 TMA Grand Master Hardware Package 2 Control computer (with mouse and keyboard) • Monitor for the control computer • Cables • Important! Microsoft Office package or license is NOT delivered with TMA Grand Master.
  • Page 14 TMA Grand Master 2.0 – User’s Guide 2 TMA Grand Master Hardware 3. Lift the main unit by its recessed transporting handles that can be found both sides of the bottom plate, underneath the side-plates of the main unit. Figure 4 – Transporting handle 4.

  • Page 15: Installation Of Tma Grand Master Software Application

    TMA Grand Master 2.0 – User’s Guide 3 Installation of TMA Grand Master Software Application 3 Installation of TMA Grand Master Software Application 3.1 Prerequisites for the TMA Grand Master Software Application Installation Prerequisites Description Hardware Recommended: Intel Pentium DualCore, 3.2 GHz, 4GB RAM...
  • Page 16 TMA Grand Master 2.0 – User’s Guide 3 Installation of TMA Grand Master Software Application 1. Install the following drivers from the 3DHISTECH Installation Disk: TMA Grand Master USB driver (CDM driver) • Camera driver – VRmagic • 2. Launch the TMAGrandMaster_2.0_RTM.exe file that you received on a 3DHISTECH Installation Disk or downloaded in a compressed file.
  • Page 17 TMA Grand Master 2.0 – User’s Guide 3 Installation of TMA Grand Master Software Application 5. Choose the installation folder for the TMA Grand Master software application. Click Browse for locating a different folder than the default one, then click Next to continue.
  • Page 18 TMA Grand Master 2.0 – User’s Guide 3 Installation of TMA Grand Master Software Application 7. The installer automatically downloads the necessary prerequisite files. Click Install to start the installation of prerequisites. 8. Click Install to continue with the installation of the TMA Grand Master application.
  • Page 19 TMA Grand Master 2.0 – User’s Guide 3 Installation of TMA Grand Master Software Application 9. After completing the installation of TMA Grand Master, click Finish to close the wizard. The relating User's Guide can be opened if you activate the Show User's Guide option in this window.
  • Page 20 TMA Grand Master 2.0 – User’s Guide 3 Installation of TMA Grand Master Software Application If VRmagic camera driver is installed for the first time, after finalizing the installation the computer must be restarted. Make sure that the Reboot now option is selected, then click Finish to complete the procedure.

  • Page 21: Operating The Main Unit

    TMA Grand Master 2.0 – User’s Guide 4 Operating the Main Unit 4 Operating the Main Unit 4.1 Switching the Main Unit On or Off Turning the Power On 1. Switch on the TMA Grand Master (first, the main switch on the back panel – see Figure 2 (2), then the one in the front –...
  • Page 22 TMA Grand Master 2.0 – User’s Guide 4 Operating the Main Unit If you start the application for the first time, a New Project window is displayed in which you must enter a name for the project. If you already have worked on projects previously and you want to create a new project, click New;...

  • Page 23: Main User Interface

    TMA Grand Master 2.0 – User’s Guide 4 Operating the Main Unit 4.3 Main User Interface With this version of TMA Grand Master offers two different views of recipient and donor block panels. Based on the loading and processing method of blocks the normal view includes thumbnails of all recipient tray positions, and as for donor blocks the selected tray is displayed in its full capacity.

  • Page 24: Inserting And Removing Blocks

    TMA Grand Master 2.0 – User’s Guide 4 Operating the Main Unit If you want to load a recipient block into the unit, click at the Unused Block(s) panel header. As for donor blocks, first click the button, then if there is a free slot on the actual tray, the door can be opened (otherwise wait until the closest tray with a free slot on it is rotated to loading position) and the block can be inserted.

  • Page 25: Cleaning Block And Pcr Cassette Preparation

    TMA Grand Master 2.0 – User’s Guide 4 Operating the Main Unit Important! To ensure the good quality of recipient blocks, make sure that the top surfaces of both, recipient and donor blocks are parallel to the plastic bottom of the cassettes, and there are no dents or cavities in the paraffin layer.
  • Page 26 TMA Grand Master 2.0 – User’s Guide 4 Operating the Main Unit Important! If TMA Grand Master cannot successfully identify the loaded PCR cassette, clean the slot area and the cassette, then insert the cassette again. For cleaning blocks use regular (37mm size) blocks only.

  • Page 27: Inserting A Block Into The Main Unit

    TMA Grand Master 2.0 – User’s Guide 4 Operating the Main Unit 4.4.3 Inserting a block into the main unit 1. Recipient block positions are displayed in a double-column on the left side of the screen. Tray positions of donor blocks are identified by six buttons (A-F), tray capacity is 10 blocks each.

  • Page 28: Removing A Block From The Main Unit

    TMA Grand Master 2.0 – User’s Guide 4 Operating the Main Unit 3. The donor/recipient block holder cylinder turns to the loading position with its appropriate side. The ID of the corresponding tray is marked with green, also the slot is lit for easier identification.

  • Page 29: Removing A Pcr Cassette From The Main Unit

    TMA Grand Master 2.0 – User’s Guide 4 Operating the Main Unit 4.4.5 Removing a PCR cassette from the main unit Warning! After removing PCR cassettes, make sure that cores are not clung to the port edges of the cover plates. Before carefully removing the cover plate of a PCR cassette, gently tap on it a couple of times to let all the samples fall entirely down to the bottom of the PCR tubes.
  • Page 30 TMA Grand Master 2.0 – User’s Guide 4 Operating the Main Unit 3. Open the tool exchanger compartment door. Figure 7 – Tool compartment 4. Loosen the drill chuck locking lever (marked with red) that fixes the drill chuck in position and remove the drill chuck by lifting it upwards.
  • Page 31 TMA Grand Master 2.0 – User’s Guide 4 Operating the Main Unit 5. Hold the front cog wheel firmly and loosen the rear cog wheel (marked with red) and turn it anticlockwise about two turns (360-720 degrees), then remove the drill bit from the drill chuck.
  • Page 32 TMA Grand Master 2.0 – User’s Guide 4 Operating the Main Unit 10. Pull out the piston, then hold the front cog wheel firmly and unscrew the rear cog wheel (marked with red) by turning it anticlockwise about two turns (360-720 degrees), and now you are able to remove the puncher.

  • Page 33: Using Tma Grand Master

    TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master 5 Using TMA Grand Master This TMA Grand Master device is designed to create TMA blocks and make it available for you to place extracted cores into PCR tubes at the same time, within the same process. With the operating software you may set processes for creating TMA blocks or PCR sampling exclusively or both.

  • Page 34: Working With Recipient Block Layouts

    TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master Make sure that the cassettes with the paraffin blocks are properly inserted into the slots. • Make sure that the drill bit is of appropriate size and that it is fixed in its position.
  • Page 35 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master 2. The Layout Editor window is displayed. 3. When you create a new pattern, you define the block layout. Type the name of your new layout into the Layout Name textbox (maximum length is 20 characters).
  • Page 36 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master 9. If you want to create a pattern in which some of the grid positions (column/row intersection) are not marked as a hole to drill, disable those positions you want TMA Grand Master to skip during drilling.

  • Page 37: Modifying A Recipient Block Layout

    TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master 5.2.2 Modifying a recipient block layout 1. Open Layout Editor window by clicking the button next to the recipient block image. 2. To modify a block layout, select the name of the desired layout from the drop-down list, then click Edit.
  • Page 38 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master In the new view for recipient block, the following options are available for you to choose from after the block thumbnail image is displayed in the Unused Block(s) panel: Click the block image then type in the ID, add •...
  • Page 39 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master Warning! Make sure that: You have selected a correct layout for the inserted block, so that • corresponding dimension values, both of the layout and the block, are identical.

  • Page 40: Inserting Donor Blocks

    TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master 5.4 Inserting Donor Blocks 1. Insert donor blocks into the first tray, positions A1-10. For more information see section 4.4.3 Inserting a block into the main unit. Warning!
  • Page 41 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master 4. If a slide or slides are found with matching IDs, their thumbnails are displayed in a window from which you can select one to use it as an overlay.

  • Page 42: Launching The Transfer Process

    TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master 5. After the barcode is identified, it will be automatically displayed in the Donor Block ID field. If barcode reading had not been previously set, the Barcode window appears.
  • Page 43 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master You can use TMA Grand Master in mixed mode (TMA and PCR) and the software makes it available for you to select some of the blocks on recipient trays as cleaning blocks.
  • Page 44 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master Important! Each donor position has its adherent PCR position (for example, A6–PCR6) and because of the maximum number of PCR markers allowed to place on a donor block is four, each PCR tube can contain up to four cores of the corresponding donor block.
  • Page 45 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master The TMA Grand Master Emergency Stop window is displayed after either clicking the Stop button, or pressing the Pause hotkey. Select an option then click OK. 4. During the process, the colors of the cores on the layout varies according to their statuses.
  • Page 46 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master 5. If punching is completed to all the selected donor blocks, remove the completed donor blocks from the tray (as described in section 4.4.4 Removing a block from the main unit) and insert new donor blocks in the tray.
  • Page 47 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master Important! Make sure when removing finished donor block (if every core selected for PCR transfer has been extracted and loaded into the PCR tube) together with the relating PCR tube.

  • Page 48: Slide Overlay

    TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master If you have exported data, and you want to exit the application, click Yes in the • displayed window. Turn the main unit and the power supply off.
  • Page 49 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master If locating a slide image on a CaseCenter server, type in the URL, then click CC connect. • Login to CaseCenter with your user name and password. Browse for the slide in the folder tree, then left click on the slide to import it.
  • Page 50 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master Flip slide image if needed by selecting one of the options Flip horizontal or Flip vertical. • Position slide image (drag, rotate, zoom) to correspond with the block image.
  • Page 51 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master Place two reference points on the block image, and refine their positions on the slide image. • If misplaced, you can delete any of them by clicking the Clear reference points button.
  • Page 52 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master If you check the Show markers checkbox, the TMA markers will be displayed. You can select • the marker category you want displayed by choosing one of the diameter values (600, 1000, 1500, or 2000) from the Markers diameter drop-down menu.
  • Page 53 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master Color RGB code 255, 0, 0 Yellow 255, 255, 0 Green 0, 128, 0 Blue 0, 0, 255 For other colors and their RGB values, please follow this link: http://www.febooti.com/products/iezoom/online-help/html-color-names-...

  • Page 54: Exporting Donor Block Data

    TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master If you have finished, press the Start button to launch the transfer process. • You can refresh the image of the label or block by pressing the Refresh button below the •...
  • Page 55 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master 3. Moving blocks from the Recipient Blocks: field to the Coherent Blocks: field one-by-one by clicking creates separate databases for each block. Click the button to move all recipient blocks to the Coherent Blocks: field.
  • Page 56 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master 6. If the software finds a Donor ID that already exists in the project, the relevant datasets will be merged together. In order to search for an ID in a specific column, the column itself can be selected manually.
  • Page 57 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master Donor block images are linked to the Donor Block IDs and stored in separate folders at the same location as the spreadsheet file. Upon clicking the donor block ID the image of the block is displayed in a separate window.
  • Page 58 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master The third sheet contains recipient block-related data table where the Donor Block IDs are shown according to their positions in the layout. Tip! If you want to import the exported data to TMA Module, you need to create a worksheet called Slides including stain types, recipient block IDs, and slide names.
  • Page 59 TMA Grand Master 2.0 – User’s Guide 5 Using TMA Grand Master 1. You can import an existing file to which additional data will be appended. Click Select File then locate file. 2. Type a database name at the relevant field if you want create a new file.

  • Page 60: Maintenance Of Tma Grand Master

    TMA Grand Master 2.0 – User’s Guide 6 Maintenance of TMA Grand Master 6 Maintenance of TMA Grand Master Follow the maintenance and cleaning tasks discussed in this section to ensure the fail-safe operation of the TMA Grand Master device.

  • Page 61: Opening The Service Compartment Door

    TMA Grand Master 2.0 – User’s Guide 6 Maintenance of TMA Grand Master 6.2.1 Opening the Service Compartment Door Service compartment might be cleaned in order to remove dust or objects dropped in. To remove the door of the service compartment do the following: 1.

  • Page 62: Replacing Fuses Of The Main Power Switch

    TMA Grand Master 2.0 – User’s Guide 6 Maintenance of TMA Grand Master 6.3 Replacing fuses of the main power switch 1. Switch off the main power supply and disconnect the power cable. 2. The fuse compartment is located next to the main power supply switch at the back side of the TMA Grand Master.

  • Page 63: Contaminated Tray Or Touch Sensor Surface

    TMA Grand Master 2.0 – User’s Guide 6 Maintenance of TMA Grand Master 5. Replace fuses, put the insert back into its mounting, and push it inwards until you hear a snap, so the lever locks. Figure 17 – Replacing the fuses Important! Use T3.15A/250V fuses only.
  • Page 64 TMA Grand Master 2.0 – User’s Guide 6 Maintenance of TMA Grand Master According to the instructions in the following message, by using a dry and clean cloth, gently • rub the bottom surface of the touch sensor (sensor is marked with red in Figure 18).

  • Page 65: Troubleshooting Tma Grand Master

    TMA Grand Master 2.0 – User’s Guide 7 Troubleshooting TMA Grand Master 7 Troubleshooting TMA Grand Master 7.1 Hardware-related issues TMA Grand Master Does Not Work at All What to do: Contact 3DHISTECH support. TMA Grand Master software stops working (due to unexpected error) What to do: Exit application, then start again.

  • Page 66: Software-Related Issues

    TMA Grand Master 2.0 – User’s Guide 7 Troubleshooting TMA Grand Master 7.2 Software-related issues HDD is inaccessible/not writable The System Administrator must ensure accessibility by the control software to some folders on the computer. For instance, write access must be granted to C:\ProgramData\3DHISTECH\TMAGrandMaster folder, its sub-folders/files, and in addition, enable the software to modify registry logs.

  • Page 67: Cassette And Block Dimensions

    TMA Grand Master 2.0 – User’s Guide 8 Cassette and Block Dimensions 8 Cassette and Block Dimensions The dimensions of the plastic cassettes suitable for TMA Grand Master are as follows: 29 mm (H) x 41 mm (W) x 6 mm (D) •...

  • Page 68: Technical Description Of The Hardware

    TMA Grand Master 2.0 – User’s Guide 9 Technical Description of the Hardware 9 Technical Description of the Hardware 9.1 Physical Dimensions and Weight Name of the Unit Dimensions in mm* Weight in kg* (Width x Depth x Height) TMA Grand Master main unit 807×505×444...

  • Page 69: Objective And Cameras

    TMA Grand Master 2.0 – User’s Guide 9 Technical Description of the Hardware 9.3 Objective and Cameras Component Description Objective 16 mm focal length Camera type VR Magic Camera resolution 3,14 Million Pixel 9.4 Ambient Conditions Condition Suitable Range Shipment (packed) Ambient temperature -40°C to +70°C...

  • Page 70: Operating Data

    TMA Grand Master 2.0 – User’s Guide 9 Technical Description of the Hardware 9.5 Operating Data Category of operating environment closed room facilities Electrical protection class Internal protection degree IP 20 Electrical safety in compliance with EN 61010-1 (IEC 61010-1) and taking CSA &...

  • Page 71: Related Documents

    TMA Grand Master 2.0 – User’s Guide Related documents Related documents Pannoramic Viewer 1.15.3 User’s Guide TMA Module 1.16 User’s Guide November 5, 2013 – Rev. 1 3DHISTECH Ltd. 71(72)

  • Page 72: Index

    TMA Grand Master 2.0 – User’s Guide Index Index Barcode............................41, 42 Block............................43, 47 Donor block....8, 9, 13, 27, 28, 33, 40, 42, 43, 44, 45, 46, 47, 48, 51, 54, 56, 57, 58, 63, 65 Drill................9, 12, 28, 29, 30, 31, 33, 34, 35, 36, 60, 67, 68 Excel............................13, 56...

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