3D Histech Pannoramic 250 FLASH II User Manual page 92

Pannoramic 250 FLASH II 1.18.1 User's Guide
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This step is recommended when using FL mode, as the application is
less capable of finding the specimen based on the specimen threshold
level.
Figure 65 – Scan everything within marked area option is selected / not selected
General threshold options
Remove coverslip – If selected, the edges of the coverslip are not detected as specimen to
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be scanned.
May 29, 2014 – Rev. 1
Scan area inside the marker – If the specimen cannot be identified as required for
digitization, (for example, weak contrast), it can help to encircle completely the
specimen with a black marker pen. You have to draw a full circle with the pen to
enable the detection. Marker pen detection will make specimen detection easier by
limiting the scanned area.
If this option is selected, only the area encircled with a marker pen will be scanned.
You can define the threshold level by changing the threshold value in the Marker
threshold level selection box in the range of 0-255. This sets the sensitivity of the
specimen detection inside the marker area. To set the desired threshold value
manually, change the setting in the Specimen threshold level selection box in the
range of 0-255. The default value is 35.
To scan everything within the marked area, check the Scan everything within
marked area selection box.
Note:
Warning!
Do not use Remove coverslip function on TMA slides! The layout of TMA
slides may mislead the detecting algorithm.
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