3D Histech Pannoramic 250 FLASH II User Manual page 67

Pannoramic 250 FLASH II 1.18.1 User's Guide
Live
Click
field. This option has no effect on a running digitization process.
Focus
To define focus limits, select Limit focus range. Set the upper and lower limits by selecting
the desired value in the selection boxes. This is useful if Auto Focus does not find the focus
on the specimen, but on the coverslip. Also, this makes the focusing sequence faster.
By using Set Current, the Lower limit value is set to -32 of the current focus value and the
Upper limit value is set to +32 of the current focus value.
Use the two arrow buttons to focus manually. This is useful when you are interested in an
area that remained blurry with Auto Focus (for example, the specimen is thicker in that
area). This does not influence the way slides are digitized in Automatic mode. The manual
focus is mainly for setup purposes, not for using the product as a traditional microscope.
Define the manual focus step size by adjusting the Step size slider or search for the sharpest
focus on the current field of view automatically by clicking
Multilayer mode
Select Single layer option if you want to scan a single layer of the slide.
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By choosing the Extended focus option, the software selects the sharpest image from each
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focus level for each image field, and combines them into one single image. Therefore those
parts that were blurry in auto/manual focus will also be sharp. This method guarantees the
maximum depth of sharpness, however, it reduces digitization speed.
May 29, 2014 – Rev. 1
to display a live image of the current specimen in the Live image display
Figure 48 – Focus level and step size
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4 Software Description
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