3D Histech Pannoramic 250 FLASH II User Manual page 91

Pannoramic 250 FLASH II 1.18.1 User's Guide
Use threshold
Brightfield preview – a normal type of illumination in the microscope for scanning the slide.
•
This option is not recommended in Fluorescent manual mode.
Darkfield preview – the preview image is created with transmitted Darkfield illumination.
•
The image is created within a minute.
Figure 64 – Brightfield and Darkfield preview images of the same slide
•
•
May 29, 2014 – Rev. 1
Scan specimen with auto threshold – This automatically detects the specimen in
the preview image.
Important!
Make sure that the result of detection is acceptable for you. Otherwise set
the threshold value manually or append missing areas with the help of
Scanned function located at the preview toolbar.
Scan specimen with user set threshold – This sets the sensitivity of the specimen
detection. To set the desired threshold value manually, change the setting in the
Specimen threshold level selection box in the range of 0-255. The default value is
35. Smaller values result in a more sensitive scanning, meaning that more areas will
be digitized, however, this may also include specks or other imperfections of the
slide. Greater values result in a less sensitive scanning, but it is possible that some
of the smaller specimen areas will not be digitized.
3DHISTECH Ltd.
4 Software Description
91(137)