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Pannoramic 250 FLASH II 1.18.1 User's Guide
4.4.4 Focus panel
Focus
You can define focus limits by selecting Limit focus range. You can set the upper and lower
limits by selecting the desired value in the selection boxes. This is useful if focusing was not
targeted on the specimen, but on a spot on the coverslip.
Multilayer mode
Select Single layer option if you want to scan a single layer of the slide.
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By choosing the Extended focus option, the software selects the sharpest image from each
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focus level for each image field, and combines them into one single image. Therefore those
parts that were blurry in auto/manual focus will also be sharp. This method guarantees the
maximum depth of sharpness, however, it reduces digitization speed.
May 29, 2014 – Rev. 1
Figure 54 – Focus panel
Figure 55 – Focus level and step size
3DHISTECH Ltd.
4 Software Description
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Troubleshooting
