Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 93

Variable wavelength detectors
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5.6.1.1 Data Collection Rate
The data collection rate is the number of data points per second (Hz) that Chromeleon collects
from the detector and stores as raw data. The number of data points per second generated by
the detector electronics depends on the detector version. Data can be generated with a
maximum collection rate of 100 Hz. (When the VWD-3400RS is operated from
Chromeleon 7.1 or later, data can be generated with a maximum collection rate of 200 Hz.)
In general, each peak should be defined by at least 20 data points. This is especially
required to achieve a good peak area precision. For chromatograms with co-eluting peaks
or low signal-to-noise ratios, 40 data points per peak is recommended.
If all peaks are relatively wide, select a slower data collection rate (for example, 1.0 Hz).
If any peaks of interest are less than a few seconds, select a faster data collection rate (for
example, 10.0 Hz).
If the data collection rate is too slow, the start points and end points of peaks will not be
determined accurately. If the collection rate is too fast, data files may occupy excessive
disk space and post-run analyses may require more processing time.
Operating Instructions
VWD-3100 and VWD-3400RS Variable Wavelength Detectors
Fig. 26: UV Options page in the program wizard
UltiMate 3000 Series:
Page 85

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