Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 135

• Tubing connections between the adapter block and the flow cell are installed at the
factory and should not be opened by the user.
• Capillary connections between the column and flow cell should be as short as possible to
avoid peak broadening effects due to excessive dead volume.
The following flow cells are available for the detector:
Part No. Description
6074.0200 Analytical flow cell
(cell volume: 11 µL, cell material: PEEK, path length: 10 mm, pressure limit: 5 MPa)
6074.0250 Analytical flow cell
(cell volume: 11 µL, cell material: stainless steel, path length: 10 mm,
pressure limit: 12 MPa)
6074.0300 Semi-micro flow cell
(cell volume: 2.5 µL, cell material: PEEK, path length: 7 mm, pressure limit: 5 MPa)
6074.0360 Semi-micro flow cell
(cell volume: 2.5 µL, cell material: stainless steel, path length: 7 mm,
pressure limit: 12 MPa)
6074.0270 Nano flow cell
(cell volume: 3 nL, cell material: fused silica, path length: 10 mm*,
pressure limit: 20 MPa)
6074.0280 Capillary flow cell
(cell volume, 45 nL, cell material: fused silica, path length: 10 mm*,
pressure limit: 20 MPa)
6074.0290 Mic flow cell (split flow)
(Cell volume: 180 nL, cell material: fused silica, path length: 10 mm*, pressure limit:
20 MPa)
6074.0320 Semipreparative flow cell
(cell volume: 0.7 µL, cell material: PEEK, path length: 0.4 mm, pressure limit: 10 MPa)
* There is no exact definition of the light path in capillary flow cells as is for conventional flow cells . Therefore, you should not use
this value for calculating the absorption coefficient or the detector sensitivity according to the Lambert-Beer law.
In addition, the following is available from Thermo Fisher Scientific for the use of the
detector in an UltiMate 3000 XRS system:
Part No. Description
6074.0285 UV monitor for the XRS system, ultra-low dispersion
(cell volume: 45 nL, cell material: fused silica, path length: 10 mm*,
pressure limit: 30 MPa)
Recommended for the use in an UltiMate 3000 XRS system in combination with a mass
spectrometer. For information about the UV monitor, refer to section 2.8 (→ page 28).
* There is no exact definition of the light path in capillary flow cells as is for conventional flow cells . Therefore, you should not use
this value for calculating the absorption coefficient or the detector sensitivity according to the Lambert-Beer law.
Installation and removal of the UV monitor is the same as for flow cells without adapter block
(→ sections 7.4.3.1 and 7.4.3.3).
Operating Instructions
VWD-3100 and VWD-3400RS Variable Wavelength Detectors
UltiMate 3000 Series:
Page 127