Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 154

UltiMate 3000 Series:
VWD-3100 and VWD-3400RS Variable Wavelength Detectors
Functional Group
Aldehyde
Amine
Anthracene
Azido
Azo
Benzene
Bromide
Carboxyl
Diphenyl
Disulfide
Esters
Ether
Iodide
Isoquinoline
Ketone
Naphthalene
Nitrate
Nitrite
Nitroso
Olefins
Oxalic Acid
Pyridine
Quinoline
Thioether
Thioketone
Thiol
Table 2: UV absorption wavelengths of various chromophores
Page 146
Chromophore
–CHO
–NH2
>C=N–
–N=N–
–Br
–COOH
–S–S–
–COOR
–O–
–I
>C=O
–ONO2
–ONO
–N=O
C=C–
HOOC–COOH
–S–
>C=S
–SH
Wavelength (nm)
280-300
195
252
375
190
285-400
202
255
208
200-210
246
194
255
205
185
260
218
266
317
270-280
220
275
312
270 (shoulder)
220-230
300-400
302
185
250
195
251
227
270
314
194
215
205
195
Operating Instructions