Loading Ars - Procleix TIGRIS Operator's Manual

S 2
ECTION

Loading ARs

1. Select a new set or previously loaded matched set of ARs.
ARs that have been previously loaded on the PROCLEIX
loaded as part of a "matched set". The system groups a set of ARs the first time that it
scans their barcodes during the inventory process. They are then required to be run as
a set in all subsequent worklists. Bottles belonging to a matched set cannot be
swapped with bottles in other sets of ARs. The System will not validate AR inventory if
the bottles in a previously used matched set were not kept together. Use the AR Report
to identify bottle IDs belonging to a matched set (see "Assay Reagent Status Report" on
page 6-2).
A worklist can only use one set of ARs. In other words, the AR quadrant cannot be
switched in the middle of assay processing.
Label the reagent bottles (Target Capture Reagent, Probe Reagent, Amplification
Reagent, Enzyme Reagent, Selection Reagent and Target Enhancer Reagent [if
applicable—PROCLEIX ULTRIO Plus
bottles belonging to a set.
Discriminatory Probe Reagents can be run with any "matched set" of ARs (Amplification
Reagent, Enzyme Reagent, Probe Reagent, Selection Reagent, TCR and Target
Enhancer Reagent [PROCLEIX ULTRIO Plus
2. Verify that all ARs are free of precipitates.
3. On the Upper Bay Inventory panel of the Inventory Status screen, click Load.
The AR Door unlocks, and a Load Assay Reagents window opens.
4. Remove and discard the caps from the AR bottles.
5. Load the prepared Amplification Reagent, Enzyme Reagent, Probe Reagent, and
Selection Reagent into the AR Carousel:
a) Open the AR Door and remove the AR Carousel Cover. While holding the AR
Carousel Cover in one hand, remove the AR Carousel. Place the AR Carousel on
an absorbent pad on a clean work surface, and replace the AR Carousel Cover
back into the AR Bay for storage.
Caution: To
Amplification and HPA Pipettors when removing the AR Carousel and/or Cover.
b) Visually inspect each reagent for foam and/or bubbles.
Caution: Inspect all packaged reagents, fluids, and consumables for damage
before use. Inspect Assay Reagents for precipitate or foam. Avoid creating foam
when handling reagents. Do not use reagents with precipitate or foam.
c) The AR Carousel quadrants are numbered 1–4. Each position within the quadrant is
labeled with the first letter of each reagent type and the quadrant number. Slide
each AR bottle into its appropriate position in the first available quadrant of the AR
Carousel.
6. If running the PROCLEIX ULTRIO Plus
quadrant 2. Load into Position 1 (left position) for use with ARs loaded in quadrant 1;
Position 2 (right position) for use with ARs loaded in quadrant 3. (See Figure 1.7 on
page 1-14.)
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Part # 902649EN Rev. A
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Assay only]) with the date and initials to identify
prevent contamination
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Assay only]) within each master lot.
or misalignment,
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Assay, load Target Enhancer Reagent into
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PROCLEIX TIGRIS System Operator's Manual
Volume I: Operating the Instrument
TIGRIS System must be
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