Sample Requirements - Procleix TIGRIS Operator's Manual

Sample Requirements

Sample Quality
All sample tubes must contain sufficient liquid for processing, and must be free of undissolved
particles, clots, and foam. Specimens with visible precipitates or fibrinous material should be
clarified according to instructions in the appropriate PROCLEIX
Volume Requirements
The minimum specimen pipetting volume is 500 μL. Up to ten replicates can be tested from
each sample tube; each replicate is a 500 μL aliquot. Calibrators and PROCLEIX
Controls have a fixed number of replicates that is set in the software. Attempts to pipette from
the sample tube beyond what is described here can lead to insufficient volume errors.
Processing a sample with less than the minimum sample volume may result in errors. The
minimum sample volume includes both the sample pipetting volume and the dead volume.
Dead Space Volume Requirement
For samples in tubes that do not contain red cells or a gel separator, the dead space volume
requirements must be taken into account. Dead space volume is defined as the volume of
sample in a tube that must be present in order for the system to initiate pipetting.
For example, the dead space volume for a 12 mm or 13 mm diameter round-bottom tube is
500 µL. Each sample of this type must have at least the required specimen pipetting volume of
500 µL, plus the dead space volume of 500 µL of plasma. Refer to the table below for minimum
specimen volume requirements.
Tube Diameter
12 mm - 13 mm
16 mm
Buffer Zone Requirement
For samples in tubes that contain cells, a layer of debris after centrifugation, or a gel separator,
a minimum volume of plasma defined as a buffer zone volume must exist between the cell,
debris, or gel interface and the PROCLEIX TIGRIS System's pipette tip.
For example, the buffer zone required for a 12 mm or 13 mm diameter round-bottom tube is
200 µL. Each sample of this type must have at least the required specimen volume of 500 µL
plus the buffer zone volume of 200 µL of plasma. Refer to the table below for minimum
specimen volume requirements.
Tube Diameter
12 mm - 13 mm
16 mm
®
PROCLEIX TIGRIS System Operator's Manual
Volume I: Operating the Instrument
Dead Space Volume
500 µL
1000 µL
Buffer Zone Volume
200 µL
400 µL
I
NTRODUCTION AND
®
Assay package insert.
Total Volume Requirement
1000 µL
1500 µL
Total Volume Requirement
700 µL
900 µL
P D
RODUCT ESCRIPTION
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TIGRIS
1-25
Part # 902649EN Rev. A