Including The Asm Phase To The 2D Gradient - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

5 Method Parameters
Method Development of Active Solvent Modulation (ASM)
Including the ASM phase to the
Figure 71
Gradients that were programmed for the second dimension originally without
ASM Valve must be shifted by the delay caused by this dilution during the ASM
phase such that the analytical gradient starts after the ASM phase.
If the ASM phase takes for example 0.41 min (based on selected ASM capillary,
flush factor and
without ASM.
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2D-LC - User Guide
Programming the
2
D flow rate), all times are shifted compared to a
Gradient ends later and the gradient stop time is increased by 0.41 min
2
D Cycle time is increased accordingly
One line is added to the gradient table for the ASM phase
All times for the analytical gradient are shifted by 0.41 min.
This is true for shifted gradient steps as well (if applicable).
2
D gradient table (example)
2
D gradient
2
D gradient
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