Set 2D-Lc Mode Multiple Heart-Cutting - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

5 Method Parameters
Method Parameters Multiple Heart-Cutting and High Resolution Sampling
2D-LC

Set 2D-LC Mode Multiple Heart-Cutting

Setting the mode has the following consequences:
•
2D-LC - User Guide
Heart-Cutting:
A relevant volume of the 1st dimension is cut off and injected onto the 2
dimension column using the pump in the 2
nd
injected on the 2 column is either defined by a peak trigger or by a time
window. When heart cutting starts, a loop is filled with the peak of interest.
Then the injection on the 2
dimension pump.
nd
dimension. The volume to be
nd
dimension starts running the gradient of the 2
nd
nd
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