15
Theoretical Background
2nd dimension as detector
nd
2
Functionally the second dimension of 2D-LC operates like a chemically sensitive
detector for the peaks that elute from the first dimension column. Thus, 2D-LC
may be understood as a three step process:
•
•
•
Figure 124 Diagram of instrumentation for 2D-LC
2D-LC - User Guide
dimension as detector
st
1
dimension separation (1)
st
Sampling of the 1
dimension (2)
nd
2
dimension separation and detection (3)
(1)
(2)
(3)
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