Triggering Of 2D-Lc; Concept Of Peak Triggering - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

2 Concepts of 2D-LC

Triggering of 2D-LC

Triggering of 2D-LC

Concept of Peak Triggering

Peak-triggered LC-LC
One or more peaks of the 1
the 2D-column. Further peaks eluted from the 1D-column during the 2
dimension gradient time are ignored.
Level
Figure 7
Peak-Triggered LCxLC
In peak-triggered LCxLC only peaks in the first dimension that exceed a given
level at a peak detector placed between the first dimension column and the
modulation valve will be injected onto the column in the 2
beginning of a peak triggered segment the flow will be increased from an set idle
flow rate value to the method flow rate. After the time resulting from the delay
volume between the peak detector and the loop (to be specified in the
configuration screen of the 2D-LC Acquisition software) the sampling and the
second dimension analysis will start. As soon as the peak parameters are below
the set limits, sampling and second dimension analysis will stop (again, under
consideration of the delay volume). The flow rate will return to its idle flow rate, if
set, at the end of a defined peak triggered segment. An increase in peak
dispersion might occur depending on the cell characteristics, the first dimension
set-up and the used second dimension separation mode (for example large flow
2D-LC - User Guide
st
dimension exceeding a given level are injected onto
Principles of peak-triggered LC-LC
1
D chromatogram
2
D sampling
2
D gradient
nd
nd
dimension. At the
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