Concepts Of 2D-Lc - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

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Concepts of 2D-LC

Concepts of 2D-LC
Concepts of 2D-LC
In a 2D-LC-System, Pump 1 generates the first dimension gradient. An
autosampler injects the sample and separates it by column 1. A 2D-LC Valve
(Injector) connects the first dimension to the second dimension and stores
sample peaks intermediately. These sample peaks are re-injected to the second
dimension, separated by a second column and measured by Detector 2.
Figure 2
In 2D-LC the following concepts exist:
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2D-LC - User Guide
Concept of a 2D-LC-System
Comprehensive 2D-LC (LC×LC)
In LC×LC, the total eluent from the first dimension is injected on to the column
in the second dimension.
Heart-cutting 2D-LC (LC-LC)
In LC-LC only parts of the eluent from the first dimension are injected on to
the column in the second dimension.
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