Principles Of Heart-Cutting 2D-Lc - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

2 Concepts of 2D-LC
Multiple Heart-Cutting and High Resolution Sampling 2D-LC

Principles of Heart-cutting 2D-LC

Multiple Heart-Cutting - Principles
Multiple Heart-Cutting - Principles
Multiple Heart-Cutting 2D-LC is a complex workflow, working on a special
algorithm for filling the sample loops and analyzing the stored cuts, based on
different criteria.
principles of the Multiple Heart-Cutting algorithm, following these principles:
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2D-LC - User Guide
"Multiple Heart-Cutting - Principles"
2
D analysis is done as soon as possible. As long as the 2
any next cut from the 1st dimension will be always directly transferred to the
nd
2 dimension and analysed. This means:
• The first 1D cut will be always directly analysed in the 2
nd
• If the 2 dimension is free, when the next
directly analysed.
nd
If the 2 dimension is occupied, the next
sample loop.
If all sample loops in the 1st dimension are occupied, the peak is lost.
A peak parking deck will always be completely analysed, before switching to
the other parking deck.
Before analyzing a new parking deck, a flush gradient is run to avoid
contamination.
Stored cuts are analysed in backwards order to avoid contamination.
on page 20 illustrates the
nd
dimension is free,
nd
dimension.
1
D cut is taken, it will also be
1
D cut will be stored in the next
20